Reversible secretome and signaling defects in diabetic mesenchymal stem cells from peripheral arterial disease patients

Chadid, Tatiana; Morris, Andrew D.; Surowiec, Alexandra; Robinson, Scott T.; Sasaki, Maiko; Galipeau, Jacques; Pollack, Brian P.; Brewster, Luke P.

doi:10.1016/j.jvs.2018.05.223

Cited by 10 publications

(7 citation statements)

References 43 publications

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“…The migrated cells on the lower surface of the membrane were fixed with 4% paraformaldehyde and stained with 0.1% gentian violet. Three to five areas were randomly selected to take pictures and count [ 33 , 34 ].…”

Section: Methodsmentioning

confidence: 99%

Conditioned medium from primary cytotrophoblasts, primary placenta-derived mesenchymal stem cells, or sub-cultured placental tissue promoted HUVEC angiogenesis in vitro

Jiang

et al. 2021

Stem Cell Res Ther

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Background As a large capillary network, the human placenta plays an important role throughout pregnancy. Placental vascular development is complex and delicate and involves many types of placental cells, such as trophoblasts, and mesenchymal stem cells. There has been no systematic, comparative study on the roles of these two groups of placental cells and the whole placental tissue in the placental angiogenesis. In this study, primary cytotrophoblasts (CTBs) from early pregnancy and primary human placenta-derived mesenchymal stem cells (hPDMSCs) from different stages of pregnancy were selected as the cell research objects, and full-term placental tissue was selected as the tissue research object to detect the effects of their conditioned medium (CM) on human umbilical vein endothelial cell (HUVEC) angiogenesis. Methods We successfully isolated primary hPDMSCs and CTBs, collected CM from these placental cells and sub-cultured placental tissue, and then evaluated the effects of the CM on a series of angiogenic processes in HUVECs in vitro. Furthermore, we measured the levels of angiogenic factors in the CM of placental cells or tissue by an angiogenesis antibody array. Results The results showed that not only placental cells but also sub-cultured placental tissue, to some extent, promoted HUVEC angiogenesis in vitro by promoting proliferation, adhesion, migration, invasion, and tube formation. We also found that primary placental cells in early pregnancy, whether CTBs or hPDMSCs, played more significant roles than those in full-term pregnancy. Placental cell-derived CM collected at 24 h or 48 h had the best effect, and sub-cultured placental tissue-derived CM collected at 7 days had the best effect among all the different time points. The semiquantitative angiogenesis antibody array showed that 18 of the 43 angiogenic factors had obvious spots in placental cell-derived CM or sub-cultured placental tissue-derived CM, and the levels of 5 factors (including CXCL-5, GRO, IL-6, IL-8, and MCP-1) were the highest in sub-cultured placental tissue-derived CM. Conclusions CM obtained from placental cells (primary CTBs or hPDMSCs) or sub-cultured placental tissue contained proangiogenic factors and promoted HUVEC angiogenesis in vitro. Therefore, our research is helpful to better understand placental angiogenesis regulation and provides theoretical support for the clinical application of placental components, especially sub-cultured placental tissue-derived CM, in vascular tissue engineering and clinical treatments.

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Section: Methodsmentioning

confidence: 99%

Conditioned medium from primary cytotrophoblasts, primary placenta-derived mesenchymal stem cells, or sub-cultured placental tissue promoted HUVEC angiogenesis in vitro

Jiang

et al. 2021

Stem Cell Res Ther

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“…Bone marrow has been investigated in most studies as the standard source of MSCs/progenitors contributing to bone repair in vivo (Park et al, 2012; Zhou et al, 2014; Nancarrow-Lei et al, 2017). Bone marrow MSCs have been isolated from individuals of various ages and health backgrounds (Alves et al, 2012; Chadid et al, 2018; Tencerova et al, 2019). Bone marrow MSCs are relatively rare, comprising <0.01% of the isolated mononuclear cell (MNC) fraction (Pittenger et al, 1999).…”

Section: Sources and Culture Of Mscs For Bone Regeneration Therapiesmentioning

confidence: 99%

Mesenchymal Stromal Cell-Based Bone Regeneration Therapies: From Cell Transplantation and Tissue Engineering to Therapeutic Secretomes and Extracellular Vesicles

Presen

Traweger

Gimona

et al. 2019

Front. Bioeng. Biotechnol.

105

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Effective regeneration of bone defects often presents significant challenges, particularly in patients with decreased tissue regeneration capacity due to extensive trauma, disease, and/or advanced age. A number of studies have focused on enhancing bone regeneration by applying mesenchymal stromal cells (MSCs) or MSC-based bone tissue engineering strategies. However, translation of these approaches from basic research findings to clinical use has been hampered by the limited understanding of MSC therapeutic actions and complexities, as well as costs related to the manufacturing, regulatory approval, and clinical use of living cells and engineered tissues. More recently, a shift from the view of MSCs directly contributing to tissue regeneration toward appreciating MSCs as “cell factories” that secrete a variety of bioactive molecules and extracellular vesicles with trophic and immunomodulatory activities has steered research into new MSC-based, “cell-free” therapeutic modalities. The current review recapitulates recent developments, challenges, and future perspectives of these various MSC-based bone tissue engineering and regeneration strategies.

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“…The migrated cells on the lower surface of the membrane were xed with 4% paraformaldehyde and stained with 0.1% gentian violet. Three to ve areas were randomly selected to take pictures and count [34,35].…”

Section: Huvecs Transwell Migration Assaymentioning

confidence: 99%

Conditioned medium from primary cytotrophoblasts, primary placenta-derived mesenchymal stem cells, or placental tissue promoted HUVECs angiogenesis in vitro

Hai-ying

Jiang

et al. 2020

Preprint

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Background As a large capillary network, the human placenta plays an important role throughout pregnancy. Placental vascular development is complex and delicate and involves many types of placental cells, such as trophoblasts, and mesenchymal stem cells. There has been no systematic, comparative study on the roles of these two groups of placental cells and the whole placental tissue in the placental angiogenesis. In this study, primary cytotrophoblasts (CTBs) from early-pregnancy and primary human placenta-derived mesenchymal stem cells (hPDMSCs) from different stages of pregnancy were selected as the cell research objects, and full-term placental tissue was selected as the tissue research object to detect the effects of their conditioned medium (CM) on HUVECs angiogenesis.Methods We successfully isolated primary hPDMSCs and CTBs, collected CM from these placental cells and placental tissue, and then evaluated the effects of the CM on a series of angiogenic processes in HUVECs in vitro. Furthermore, we measured the levels of angiogenic factors in the CM of placental cells or tissue by an angiogenesis antibody array.Results The results showed that not only placental cells but also placental tissue, to some extent, promoted HUVECs angiogenesis in vitro by promoting proliferation, adhesion, migration, invasion, and tube formation. We also found that primary placental cells in early pregnancy, whether CTBs or hPDMSCs, played more significant roles than those in middle and full-term pregnancy. The effect of CM from placental tissue was better than that of CM from a single placental cell type. The semiquantitative angiogenesis antibody array showed that, in placental tissue-derived CM, 18 of the 43 angiogenic factors had obvious spots, and the levels of 5 factors (including CXCL-5, GRO, IL-6, IL-8, and MCP-1) were the highest. The levels of these 18 angiogenic factors in placental tissue were higher than those in any single placental cell type.Conclusions CM obtained from placental cells (primary CTBs or hPDMSCs) or placental tissue contained proangiogenic factors and promoted HUVECs angiogenesis in vitro. Therefore, our research is helpful to better understand placental angiogenesis regulation and provides theoretical support for the clinical application of placental components, especially placental tissue-derived CM, in vascular tissue engineering and clinical treatments.

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Reversible secretome and signaling defects in diabetic mesenchymal stem cells from peripheral arterial disease patients

Cited by 10 publications

References 43 publications

Conditioned medium from primary cytotrophoblasts, primary placenta-derived mesenchymal stem cells, or sub-cultured placental tissue promoted HUVEC angiogenesis in vitro

Conditioned medium from primary cytotrophoblasts, primary placenta-derived mesenchymal stem cells, or sub-cultured placental tissue promoted HUVEC angiogenesis in vitro

Mesenchymal Stromal Cell-Based Bone Regeneration Therapies: From Cell Transplantation and Tissue Engineering to Therapeutic Secretomes and Extracellular Vesicles

Conditioned medium from primary cytotrophoblasts, primary placenta-derived mesenchymal stem cells, or placental tissue promoted HUVECs angiogenesis in vitro

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