2013
DOI: 10.1002/glia.22507
|View full text |Cite
|
Sign up to set email alerts
|

Reversible reactivity by optic nerve astrocytes

Abstract: Reactive astrocytes are typically studied in models that cause irreversible mechanical damage to axons, neuronal cell bodies, and glia. Here, we evaluated the response of astrocytes in the optic nerve head to a subtle injury induced by a brief, mild elevation of the intraocular pressure. Astrocytes demonstrated reactive remodeling that peaked at three days, showing hypertrophy, process retraction and simplification of their shape. This was not accompanied by any significant changes in the gene expression profi… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
2
1

Citation Types

5
87
0

Year Published

2014
2014
2019
2019

Publication Types

Select...
7
1

Relationship

1
7

Authors

Journals

citations
Cited by 92 publications
(92 citation statements)
references
References 59 publications
(100 reference statements)
5
87
0
Order By: Relevance
“…These morphological properties are quite similar to the morphology of optic nerve head astrocytes in vivo that was observed from transverse sections of the optic nerve head of B6.hGFAPpr-EGFP mice, in which astrocytes express EGFP (Fig. 1C), as well as that was described in previous studies (Sun et al, 2009, 2010; Sun et al, 2013). For comparison, a microglial cell from a B6.129P-Cx3cr1 tm1Litt /J mouse was imaged in the same way (Fig.…”
Section: Detailed Methodssupporting
confidence: 87%
See 2 more Smart Citations
“…These morphological properties are quite similar to the morphology of optic nerve head astrocytes in vivo that was observed from transverse sections of the optic nerve head of B6.hGFAPpr-EGFP mice, in which astrocytes express EGFP (Fig. 1C), as well as that was described in previous studies (Sun et al, 2009, 2010; Sun et al, 2013). For comparison, a microglial cell from a B6.129P-Cx3cr1 tm1Litt /J mouse was imaged in the same way (Fig.…”
Section: Detailed Methodssupporting
confidence: 87%
“…These two genes were chosen to validate the method because the changes in gene expression for both were known from prior experiments. GFAP is significantly (though not dramatically) up-regulated on the protein level after optic nerve crush (Sun et al, 2013). Both GFAP and vimentin are not significantly differentially regulated in whole optic nerve head tissue after injury when assayed by microarray (Qu and Jakobs, 2013), and are up-regulated slightly (~1.25-fold) when assayed by qPCR.…”
Section: Detailed Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Astrocytes are positioned along the connective tissues of the ONH, including the laminar beams in the primate ONH [10]. Astrocytes exhibit multiple extensions that enter and unsheathe axon bundles [7, 11, 12]. These astrocyte extensions further couple the meningeal vasculature to axons [13, 14], and are involved in neural development and synapse formation [15], metabolic and ionic support of ONH axons [1618], facilitate mitochondrial transcellular degradation from retinal ganglion cell axons [19], and phagocytosis of myelin segments within the optic nerve [20, 21].…”
Section: Introductionmentioning
confidence: 99%
“…Several laboratories have also used short-term elevations in IOP, from 30 min to several hours, as a unique approach to determine ONH and retinal responses to IOP (Abbott et al, 2014; Balaratnasingam et al, 2007, 2008; Sun et al, 2013). Others have combined experimental glaucoma with electroretinography (ERG) as a non-invasive test to measure the impact of short term IOP exposures on the ONH and retina, and have used this system to determine the effects of age, dietary restriction and mitochondrial abnormalities on their response (Kong et al, 2011, 2012).…”
Section: Introductionmentioning
confidence: 99%