Reversible Norepinephrine Binding to Rabbit Myometrium: Relationship to Sites of Known Biological Significance

Roberts, James M.; Keller, David W.; Goldfien, Alan

doi:10.1159/000301390

Cited by 2 publications

(1 citation statement)

References 11 publications

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“…The number of receptors could then be quantified, and correspondent changes could be recorded. 6,7 It was a novel area of investigation at the time, and little was known about the effectiveness of this strategy, either in conducting the study or analyzing the data. However, Jim's mentor, Dr Alan Goldfien, an endocrinologist and a founding member of CVRI, was an expert on catecholamines and adrenergic receptors, which allowed them to avoid several confounders, such as autoxidation.…”

Section: The First Steps In a Research Careermentioning

confidence: 99%

Giants in Obstetrics and Gynecology Series: A profile of James M. Roberts, MD

Romero

2019

American Journal of Obstetrics and Gynecology

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D r James Roberts has made seminal contributions to the understanding of the physiology of pregnancy and, in particular, to the mechanisms of disease responsible for preeclampsia. His research includes endothelial cell dysfunction, oxidative stress, and other factors that play a role in the pathogenesis of preeclampsia; the use of antioxidant therapy to prevent the syndrome; identification of clinical subtypes of preeclampsia; and assessment of the long-term cardiovascular risk of the disorder. Dr Roberts is currently an investigator at the Magee-Womens Research Institute and a Professor of Obstetrics Gynecology and Reproductive Sciences, Endocrinology, and Clinical and Translational Medicine at the University of Pittsburgh. He was the founding Director of the Magee-Womens Research Institute and formerly the Elsie Hilliard Hillman Chair in Women's and Infants' Health Research at the University of Pittsburgh. For his many original contributions to the understanding of preeclampsia and other obstetrical syndromes, Dr Roberts is herein recognized as a "Giant in Obstetrics and Gynecology". The Path To Medicine The second of four children, Jim was born in 1941 and raised in a blue-collar household in Taylor, Michigan. His father, originally from Pennsylvania, moved to the Detroit area during the Great Depression to work at the Ford Motor Company, first as a timekeeper, then as an accountant. Jim envisioned a college education, the first member of his family to follow an academic path. To make this dream possible, he entered a scholarship competition sponsored by Ford, for which he had to submit an essay about what he would do with his degree. "I wrote that I wanted to be a physician, because that's the only thing I knew people did after attending college," he said. The recipient of this four-year scholarship, Jim began his undergraduate journey in 1959 at the University of Michigan in Ann Arbor, Michigan, where he would spend his next 12 years. When I asked Jim about his decision to go into obstetrics and gynecology, he replied, "it was fate." Recognizing that the Ford scholarship would not fully cover medical school, he sought other financial aid options. This brought him into contact with Dr John Gosling, the Dean overseeing student loans at the University of Michigan, who happened to be an obstetrician and gynecologist. Dr. Gosling offered Jim the opportunity to see patients with him and involved him in bibliographic research projects, introducing him to the academic side of medicine. Once he began to visit patients with Dr. Gosling, Jim decided "there could not be anything more fun than taking care of patients and being paid to learn." Already enthusiastic about academics, Jim became hooked on obstetrics. Although the field was not necessarily what he had set out to study, he was glad the path opened up for him. He remembers his lifelong mentor and friend as a "remarkable individual, a true character," who delivered Jim's first child.

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Section: The First Steps In a Research Careermentioning

confidence: 99%

Giants in Obstetrics and Gynecology Series: A profile of James M. Roberts, MD

Romero

2019

American Journal of Obstetrics and Gynecology

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Identification of Beta-Adrenergic Binding Sites in Rabbit Myometrium

et al. 1977

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Rabbit uterine muscle may contract or relax with adrenergic stimulation depending on the hormonal milieu. This difference in contractile activity has been shown to be due to alteration of adrenergic response between alpha-adrenergic (contraction) and beta-adrenergic (relaxation). When rabbits are treated with estrogen followed by progesterone, norepinephrine produces myometrial relaxation. This effect is blocked by propranolol, indicating that it is mediated by beta receptor activation. A subcellular preparation of this myometrium has adenylate cyclase activity that can be stimulated by isoproterenol + guanyl-5'-yl-imidodi-phosphate (Gpp(NH)p). The radioligand [125I]iodohydroxybenzylpindolol binds to the same preparation. The binding is rapid, 80% maximal in 10 min, and readily reversible (t1/2 = 5 min). The binding is high affinity (Kd = 0.12 nM), low capacity (15 fmol/mg protein), and is to a single class of binding sites. Binding is competed for stereoselectively by beta adrenergic agonists and antagonists. The competition of beta adrenergic agonists for binding, isoproterenol = ritodrine greater than epinephrine greater than norepinephrine, is consistent with interactions at a beta2-adrenergic receptor.

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Reversible Norepinephrine Binding to Rabbit Myometrium: Relationship to Sites of Known Biological Significance

Cited by 2 publications

References 11 publications

Giants in Obstetrics and Gynecology Series: A profile of James M. Roberts, MD

Giants in Obstetrics and Gynecology Series: A profile of James M. Roberts, MD

Identification of Beta-Adrenergic Binding Sites in Rabbit Myometrium

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