Reversible Loading of Nanoscale Elements on a Multicomponent Supramolecular Polymer System by Using DNA Strand Displacement

Abstract

Nucleic acids are excellent building blocks to enable switchable character in supramolecular polymer materials because of their inherent dynamic character and potential for orthogonal self‐assembly. Herein, DNA‐grafted squaramide bola‐amphiphiles are used in a multicomponent supramolecular polymer system and it is shown that they can be addressed by DNAlabeled gold nanoparticles (5 and 15 nm) through sequence complementarity. These nanoparticles can be selectively erased or rewritten on‐demand by means of DNA‐strand displacement.

Squaramide-Based Supramolecular Materials for Three-Dimensional Cell Culture of Human Induced Pluripotent Stem Cells and Their Derivatives

“… 29 , 30 Commonly used hydrogen bonding synthons include amides, 31 thioamides, 32 ureas 33 , 34 and thioureas. 35 Despite their extensive use in the areas of bioconjugation, 36 medicinal chemistry, 37 catalysis, 38 and anion recognition, 39 squaramides have been explored to a far lesser extent in the materials domain, especially with respect to self-assembly, 39 − 41 with few examples reported in water, 42 − 44 and none thus far have been applied to 3D cell culture.…”

DNA-inspired oligomers: from oligophosphates to functional materials

“…5B). 30 Chemical integrity of the self-assembling unit, in this study squaramide 24, is a prerequisite for the successful integration of oligonucleotide conjugates into the supramolecular stacks. The efficient incorporation of the targeted amounts of squaramide-DNA (1 mol%) oligomers was confirmed for the co-assembled materials by the sequence-specific binding to the protruding oligonucleotide strands.…”

The Versatility of Squaramides: From Supramolecular Chemistry to Chemical Biology

“…and demonstrated their capacity to act as effective multi-component supramolecular polymers capable of recognizing DNA-labeled gold nanoparticles (Figure 63). 139 Through TEM analysis, gel electrophoresis, fluorescence quenching, and thermal denaturation experiments, they observed reversible loading and exchange of AuNPs by orthogonal self-assembly. Moreover, the recognition event could be addressed in a programmable and reversible fashion through the orthogonal self-assembly of varying DNA strands.…”