Reversible Inhibition of Mitosis in Lymphocyte Cultures by Non-Viable Mycoplasma.

Abstract

more potent than the parent in the Clauberg test, was less potent as an estrone antagonist; thus the ratio was grossly reduced. Incorporation of a double bond at the 9-10 position of 1 7~-chloroethynyl-13p-ethyl-1 7-hydroxygon-4-en-3-one resulted in decreases of both activities; however, since the decrease in progestational effect was greater, the ratio was considerably increased. 1 7~-Chloroethynyl-13fl-ethyl-l7-hydroxygon-4,9-dien-3-one was less potent in the Clauberg test, but more potent in the anti-estrogenic assay than the lower homologue. Thus the ratio of potencies was decreased.13~-Ethyl-17-hydroxy-l7~~-methylgon-4-en-3-one was more anti-estrogenic and less progestational than its lower homologue. 1 7~-E thy1 -1 7-hydroxy-13p -me thylgon-4 -en-3 -one (norethandrolone) was less potent in both assays than its 18-homologue (norbolethone) , which because of a greater anti-estrogenic potency had a higher ratio. Incorporation of a double bond in the B-ring of norbolethone to form 13p, 1 7a-diethyl-17-hydroxygon-4,9dien-3-one did not alter progestational potency, whereas the anti-estrogenic potency and the ratio were decreased, These data fail to indicate any direct correlation of progestational and anti-estrogenic effects. Although both these biological activities are often present in a single molecule, they do not appear to be necessary correlates.Summary. The progestational (Clauberg) and anti-estrogenic (mouse vaginal smear) potencies of various A4-3-oxosteroids are compared. The ratio anti-estrogenic potency,' progestational potency varies from 0.1 to 85, indicating that there is no necessary correlation between these parameters of steroid action.

Clastogenic effects of different Ureaplasma urealyticum serovars on human chromosomes

“…Lymphocytes were obtained from a single donor and 11 serotypes of Ureaplasma urealyticum (1,2,3,5,6,7,8,9,10,11,12) were obtained from the American Type Culture Collection. Four supernatants obtained by centrifugation of the culture broths of serotypes 2, 3, 7 and 10 at 14,200 g for 40 min were also assayed.…”

“…In contrast, the clastogenic effects observed with serotypes 1, 7 and 12 varied according to the concentration employed in the test. Mitotic alterations were observed in Ureaplasma urealyticum serotypes 5,6,7,8,9, 11 and 12. Chromatid gaps (53.0%) and chromatid breaks (13.9%) were the most frequent types of alterations observed.…”

“…Different mycoplasma species can lead to different changes in a certain cell, while the same mycoplasma species can lead to different changes in cells of the same type belonging to different host species. Chromosomal aberrations, mitotic inhibition and stimulation and various other cytopathic effects have been observed (5)(6)(7)(8)(9).…”

Development of IL-2 Independent Feline Lymphoid Cell Lines Chronically Infected with Feline Immunodeficiency Virus: Importance for Diagnostic Reagents and Vaccines

“…Mycoplasma species are well known for their ability to affect the cell growth rate and the production of virus in cell lines [23,24], How ever, our results demonstrate that our cell lines are free of mycoplasma contamination, an important consideration in the light of recent studies which suggest that these agents may be cofactors in the pathogenesis of AIDS [25,26].The DNA fluorochromestain ing assay used in our study is an established procedure for detecting the majority of my coplasma species including Mycoplasma fermentans, the putative AIDS cofactor. Both FL-4 and FL-6 cells were negative for FeLV core protein expression by ELISA and for latent exogenous FeLV infection by PCR.…”

Comparative Mitogenic Effects of Herpes Simplex Virus and Mycoplasma on Murine Lymphocytes

“…This variability could be due to the presence of mycoplasma in the cells or viral inoculum used to propagate virus, since mycoplasmas are able to dramatically alter normal lymphocyte function. Mycoplasma infection can result in either activation or inhibition of normal lymphocyte cultures (Copperman & Morton, 1966;Barile & Leventhal, 1968). Mycoplasma infection of murine B lymphocytes causes blast transformation and results in polyclonal activation (Ginsberg & Nicolet, 1973;Biberfeld & Gronowicz, 1976;Stanbridge & Weiss, 1978), while infection of murine T lymphocytes can result in induction of lymphocyte-mediated cytotoxicity for syngeneic cells (Aldridge eta!., 1977).…”