Reversible inactivation of the dorsal hippocampus by tetrodotoxin or lidocaine: A comparative study on cerebral functional activity and motor coordination in the rat

Vasconcelos, Anne Pereira de; Klur, Sandra; Muller, Christophe; Cosquer, Brigitte; Lopez, Joëlle; Certa, Ulrich; Cassel, Jean‐Christophe

doi:10.1016/j.neuroscience.2006.05.023

Cited by 32 publications

(29 citation statements)

References 68 publications

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“…The failure to observe conditioned reinstatement in rats with transient DSUB inactivation also cannot be explained as merely a consequence of changes in the perception of cocaine effects because TTX did not alter cocaine-reinforced responding during the conditioning session. The absence of nonspecific effects is likely explained by the low dose of TTX, flow rate, and volume of injection compared to the study by Pereira de Vasconcelos et al (2006) and consistent with data showing that selective excitotoxic lesions of the DSUB or the VSUB have no effects on maintenance of cocaine self-administration in rats (Caine et al, 2001).…”

Section: Discussionsupporting

confidence: 85%

“…While reversible inactivation of the DH with TTX can impair rat motor coordination as measured in the beam-walking test (Pereira de Vasconcelos et al, 2006), in the present study, TTX microinjections in the DSUB or VSUB did not produce any overt behavioral impairments as can be inferred from the level of operant responding across the experimental groups (Figure 2: vehicle vs TTX injected animals). The failure to observe conditioned reinstatement in rats with transient DSUB inactivation also cannot be explained as merely a consequence of changes in the perception of cocaine effects because TTX did not alter cocaine-reinforced responding during the conditioning session.…”

Section: Discussioncontrasting

confidence: 58%

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The Dorsal Subiculum Mediates the Acquisition of Conditioned Reinstatement of Cocaine-Seeking

Martin‐Fardon

Ciccocioppo

Aujla

et al. 2007

Neuropsychopharmacol

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Contextual stimuli present during a single lifetime cocaine self-administration experience acquire occasion-setting actions sufficient to persistently elicit cocaine-seeking behavior in rats, with effects lasting nearly 1 year. The goal of this study was to identify neural substrates mediating the acquisition of drug-related conditioning taking place during a single cocaine self-administration experience with focus on the subicular formation, a brain site that has been implicated in associative learning relevant for conditioned reward-seeking including conditioned reinstatement. Male Wistar rats were given 2 h of response-contingent access to intravenous cocaine or saline in the presence of distinct stimuli that served as contextual stimuli associated with the availability and subjective effects of cocaine (S + ) vs saline (S À ). Before onset of the sessions, rats received bilateral microinjections of tetrodotoxin (TTX) into the ventral subiculum (VSUB) or dorsal subiculum (DSUB). Following extinction of responding by withholding cocaine, rats were subjected to reinstatement tests in which exposure to the cocaine-(but not saline) associated stimulus produced strong recovery of responding. This effect was completely abolished in rats with transient TTX inactivation of the DSUB during the conditioning session. TTX inactivation of the VSUB during conditioning did not alter the response-reinstating effects of the cocaine cue. The results suggest that functional integrity of the DSUB, but not VSUB, is critical for the acquisition of conditioned cocaine-seeking controlled by contextual stimuli under conditions where such learning occurs during a single conditioning trial.

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Section: Discussionsupporting

confidence: 85%

Section: Discussioncontrasting

confidence: 58%

The Dorsal Subiculum Mediates the Acquisition of Conditioned Reinstatement of Cocaine-Seeking

Martin‐Fardon

Ciccocioppo

Aujla

et al. 2007

Neuropsychopharmacol

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“…The specifications of the water maze and the testing procedures have been described previously (Pereira de Vasconcelos et al, 2006). The first part of training was a four trial session using a visible platform located in the South-East quadrant of the pool, starting randomly from each of the four cardinal points at the edge of the pool.…”

Section: Methodsmentioning

confidence: 99%

The Intralaminar Thalamic Nuclei Contribute to Remote Spatial Memory

Lopez¹,

Wolff²,

Lecourtier³

et al. 2009

J. Neurosci.

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Recent studies have shown that the anterior (ATN) and lateral thalamic nuclei (including the intralaminar nuclei; ILN/LT) play different roles in memory processes. These nuclei have prominent direct and indirect connections with the hippocampal system and/or the prefrontal cortex and may thus participate in the time-dependent reorganization of memory traces during systems-level consolidation. We investigated whether ATN or ILN/LT lesions in rats influenced acquisition and subsequent retrieval of spatial memory in a Morris water maze. Retrieval was assessed with a probe trial after a short (5 d, recent memory) or a long (25 d, remote memory) postacquisition delay. The ATN group showed impaired acquisition compared with the Sham controls and ILN/LT groups, which did not differ during acquisition, and exhibited no preference for the target quadrant during the recent or remote memory probe trials. In contrast, probe trial performance in rats with ILN/LT lesions differed according to the age of the memory, with accurate spatial retrieval for the recent memory probe trial but impaired retrieval during the remote memory one. These findings confirm that ATN but not ILN/LT lesions disrupt the acquisition of spatial memory and provide new evidence that the ILN/LT region contributes to remote memory processing. Thus, the lateral thalamus may modulate some aspects of remote memory formation and/or retrieval during the course of systems-level consolidation.

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“…Lidocaine hydrochloride (LIDO, 20 g in 0.3 l PBS; Sigma) was infused over 60 s; the needle was then left in place for an additional 30 s before its retraction. The choice of LIDO over other possible drugs targeting cell bodies (e.g., muscimol or 6-cyano-7-nitroquinoxaline-2,3-dione) but not the fibers en passage was based on our quite extensive experience with this drug infused in various brain areas such as the dorsal and ventral hippocampus and mPFC (Pereira de Vasconcelos et al, 2006;Klur et al, 2009;Lopez et al, 2012;Loureiro et al, 2012). In these experiments, using the 2-deoxyglucose method to map cerebral glucose metabolism (and behavioral evaluations in parallel), we showed that the LIDO diffusion area was mostly confined around the infusion site without any major changes in surrounding or in some distant brain structures (Pereira de Vasconcelos et al, 2006;Lopez et al, 2012).…”

Section: Specific Experimental Protocolsmentioning

confidence: 99%

“…The choice of LIDO over other possible drugs targeting cell bodies (e.g., muscimol or 6-cyano-7-nitroquinoxaline-2,3-dione) but not the fibers en passage was based on our quite extensive experience with this drug infused in various brain areas such as the dorsal and ventral hippocampus and mPFC (Pereira de Vasconcelos et al, 2006;Klur et al, 2009;Lopez et al, 2012;Loureiro et al, 2012). In these experiments, using the 2-deoxyglucose method to map cerebral glucose metabolism (and behavioral evaluations in parallel), we showed that the LIDO diffusion area was mostly confined around the infusion site without any major changes in surrounding or in some distant brain structures (Pereira de Vasconcelos et al, 2006;Lopez et al, 2012). In addition, the use of a drug that does not target a specific neurotransmission system appeared more appropriate to us, because an imbalance between inhibition/excitation in some thalamic nuclei might have side effects other than only inhibition including triggering seizures (Steriade, 2006).…”

Section: Specific Experimental Protocolsmentioning

confidence: 99%

The Ventral Midline Thalamus (Reuniens and Rhomboid Nuclei) Contributes to the Persistence of Spatial Memory in Rats

Loureiro¹,

Cholvin²,

Lopez³

et al. 2012

Journal of Neuroscience

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125

134

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The formation of enduring declarative-like memories engages a dialog between the hippocampus and the prefrontal cortex (PFC). Electrophysiological and neuroanatomical evidence for reciprocal connections with both of these structures makes the reuniens and rhomboid nuclei (ReRh) of the thalamus a major functional link between the PFC and hippocampus. Using immediate early gene imaging (c-Fos), fiber-sparing excitotoxic lesion, and reversible inactivation in rats, we provide evidence demonstrating a contribution of the ReRh to the persistence of a spatial memory. Intact rats trained in a Morris water maze showed increased c-Fos expression (vs home cage and visible platform groups: Ͼ500%) in the ReRh when tested in a probe trial at a 25 d delay, against no change at a 5 d delay; behavioral performance was comparable at both delays. In rats subjected to excitotoxic fiber-sparing NMDA lesions circumscribed to the ReRh, we found normal acquisition of the water-maze task (vs sham-operated controls) and normal probe trial performance at the 5 d delay, but there was no evidence for memory retrieval at the 25 d delay. In rats having learned the water-maze task, lidocaine-induced inactivation of the ReRh right before the probe trial did not alter memory retrieval tested at the 5 d or 25 d delay. Together, these data suggest an implication of the ReRh in the long-term consolidation of a spatial memory at the system level. These nuclei could then be a key structure contributing to the transformation of a new hippocampal-dependent spatial memory into a remote one also depending on cortical networks.

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Reversible inactivation of the dorsal hippocampus by tetrodotoxin or lidocaine: A comparative study on cerebral functional activity and motor coordination in the rat

Cited by 32 publications

References 68 publications

The Dorsal Subiculum Mediates the Acquisition of Conditioned Reinstatement of Cocaine-Seeking

The Dorsal Subiculum Mediates the Acquisition of Conditioned Reinstatement of Cocaine-Seeking

The Intralaminar Thalamic Nuclei Contribute to Remote Spatial Memory

The Ventral Midline Thalamus (Reuniens and Rhomboid Nuclei) Contributes to the Persistence of Spatial Memory in Rats

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