Reversible DNA micro-patterning using the fluorous effect

Abstract

We described the use of the fluorous effect as a simple and reversible immobilisation technique for DNA.

High-Throughput Amenable MALDI-MS Detection of RNA and DNA with On-Surface Analyte Enrichment Using Fluorous Partitioning

“…The plates are shelf stable for more than 18 months with no special storage conditions required, and they were also found to be reusable up to eight times each (four times on each side) after cleaning with water, 50:50 water−isopropanol, and 100% isopropanol between each use. The reusability of the F-HT-MS surface is consistent with that observed with other fluorous surfaces, 31,33 despite the extra burden of exposure to laser ablation at fixed positions. Reusability of the F-HT-MS plates is highly desirable to reduce the overall cost of an HTS campaign, particularly in comparison with other capture surfaces that are generally single use only.…”

“…28,29 Fluorous-based immobilization for array formation has been utilized with small molecules, 30,31 peptides, 32 and recently DNA. 33 In 2005 Brittain et al provided the first example of using fluorous partitioning for analyte enrichment of peptides prior to ESI-MS analysis and found the tags to be nonfragmentary and non-ion suppressive. 34 Since then, three fluorous surfaces for selective capture of fluorous-tagged peptides and carbohydrates, followed by desorption/ionization from the surface, have been reported: fluorous-modified DIOS, 35 NIMS, 20 and fluorous-modified aluminum oxidecoated slides.…”

Perfluorocarbons in Chemical Biology

“…[138,139] Fluorous-tagged DNA can be used for micropatterning in a reversible manner through five cycles of immobilization and removal. [138] Peptides can be presented for protease screening; careful design of the linker between the peptide and the fluorous tag leads to negligible enzyme inhibition. [140] Similarly, small molecules have been displayed to identify histone deacetylase inhibitors.…”

“…These works have explicitly demonstrated minimal nonspecific binding on fluorous microarrays [138,139] . Fluorous‐tagged DNA can be used for micropatterning in a reversible manner through five cycles of immobilization and removal [138] . Peptides can be presented for protease screening; careful design of the linker between the peptide and the fluorous tag leads to negligible enzyme inhibition [140] .…”

“…Since this initial report, fluorous microarrays have been used to present DNA and proteins. These works have explicitly demonstrated minimal nonspecific binding on fluorous microarrays [138,139] . Fluorous‐tagged DNA can be used for micropatterning in a reversible manner through five cycles of immobilization and removal [138] .…”