Reversible biomechano-responsive surface based on green fluorescent protein genetically modified with unnatural amino acids

Longo, Johan; Chen, Yao; Rios, César; Chau, Nguyet Trang Thanh; Boulmedais, Fouzia; Hemmerlé, Joseph; Lavalle, Philippe; Schiller, Stefan; Schaaf, Pierre; Jierry, Loı̈c

doi:10.1039/c4cc07486f

Cited by 21 publications

(21 citation statements)

References 15 publications

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“…Other dyes, which include bis(benzoxazolyl) stilbene and perylene derivatives, can be dispersed into polymeric materials during melt compounding or in solution. Further mechanochromic substances that have been used in polymeric materials are shear‐responsive photoluminescent ZnO tetrapods, CdSe–CdS tetrapod quantum dots, or force‐responsive proteins . The reader is referred to various reviews for a detailed overview of this field …”

Section: Mechanochromic Fiber‐reinforced Compositesmentioning

confidence: 99%

Self‐Reporting Fiber‐Reinforced Composites That Mimic the Ability of Biological Materials to Sense and Report Damage

et al. 2018

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Sensing of damage, deformation, and mechanical forces is of vital importance in many applications of fiber-reinforced polymer composites, as it allows the structural health and integrity of composite components to be monitored and microdamage to be detected before it leads to catastrophic material failure. Bioinspired and biomimetic approaches to self-sensing and self-reporting materials are reviewed. Examples include bruising coatings and bleeding composites based on dye-filled microcapsules, hollow fibers, and vascular networks. Force-induced changes in color, fluorescence, or luminescence are achieved by mechanochromic epoxy resins, or by mechanophores and force-responsive proteins located at the interface of glass/carbon fibers and polymers. Composites can also feel strain, stress, and damage through embedded optical and electrical sensors, such as fiber Bragg grating sensors, or by resistance measurements of dispersed carbon fibers and carbon nanotubes. Bioinspired composites with the ability to show autonomously if and where they have been damaged lead to a multitude of opportunities for aerospace, automotive, civil engineering, and wind-turbine applications. They range from safety features for the detection of barely visible impact damage, to the real-time monitoring of deformation of load-bearing components.

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Section: Mechanochromic Fiber‐reinforced Compositesmentioning

confidence: 99%

Self‐Reporting Fiber‐Reinforced Composites That Mimic the Ability of Biological Materials to Sense and Report Damage

et al. 2018

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“…The materials signaled fiber fracture as well as debonding between fibers and matrix as the forces at the interface led to an unfolding of the embedded proteins that was accompanied by a loss of its yellow fluorescence. Schaaf, Schiller, and coworkers covalently grafted genetically modified green fluorescent proteins onto a poly(dimethyl siloxane) surface and showed that uniaxial mechanical stresses resulted in a fully reversible linear decrease of the fluorescence intensity that was attributed to conformational changes of the protein . Moreover, fluorescent proteins were also employed in polymer matrices and their fluorescence signal was used to facilitate the detection of compressive stresses .…”

Section: Mechanically Induced Cleavage Of Covalent and Noncovalent Bondsmentioning

confidence: 99%

Approaches to polymeric mechanochromic materials

Calvino

Neumann

Weder

et al. 2016

J. Polym. Sci. Part A: Polym. Chem.

131

129

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Mechanochromic materials respond to mechanical stimuli with a change of their optical properties. Such materials are of interest for many technological applications and support fundamental research as they help improving the understanding of stress transfer in polymeric objects and aid in the identification of the processes that lead to mechanical failure. In this highlight, different approaches are discussed that permit the design of polymeric materials, which signal mechanical stresses through a chromic response. This highlight emphasizes materials that exhibit mechanically induced changes of their intrinsic absorption or emission properties. These responses almost exclusively originate from changes of molecular structure, conformational rearrangements, or disruption of intermolecular interactions. V C 2016 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2017, 55, 640-652

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“…171 A non-natural amino acid, pAzF, was first incorporated into two different sites of GFP. A PEG molecule was used as a cross-linker connecting a protein to a silicon surface, generating a biomechano-responsive surface.…”

Section: Other Applications Of Pegylationmentioning

confidence: 99%

Expansion of bioorthogonal chemistries towards site-specific polymer–protein conjugation

Jung

Kwon

2016

Polym. Chem.

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Polymer conjugation to proteins has been widely used to improve or expand protein properties. However, polymer conjugation to random sites of a target protein often led to a significant loss of critical protein properties. In order to overcome this, polymer conjugation to specific sites of a protein was developed using the site-specific introduction of non-natural amino acids and bioorthogonal chemistries. This review summarizes the recent advances in bioorthogonal chemistries. As the repertoire of bioorthogonal chemistries available for polymer conjugation is expanding, the site-specific polymer conjugation technique would be a valuable platform technique to design novel protein-polymer conjugates.

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Reversible biomechano-responsive surface based on green fluorescent protein genetically modified with unnatural amino acids

Cited by 21 publications

References 15 publications

Self‐Reporting Fiber‐Reinforced Composites That Mimic the Ability of Biological Materials to Sense and Report Damage

Self‐Reporting Fiber‐Reinforced Composites That Mimic the Ability of Biological Materials to Sense and Report Damage

Approaches to polymeric mechanochromic materials

Expansion of bioorthogonal chemistries towards site-specific polymer–protein conjugation

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