Reversible antifertility effect of aqueous rhizome extract of Curcuma longa L. in male laboratory mice

Mishra, Raghav Kumar; Singh, Shio Kumar

doi:10.1016/j.contraception.2009.01.001

Cited by 62 publications

(38 citation statements)

References 11 publications

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“…Because MA-10 cells express low level and activity of Cyp17a1, the enzyme to convert progesterone into 17-OH progesterone for testosterone synthesis, MA-10 cells produce progesterone as the major product during stimulatedsteroidogenesis [15]. By comparing the results obtained from primary mouse Leydig cells and MA-10 as the cells, we demonstrated that curcumin not only reduces serum testosterone level in vivo [8], but also cellautonomously suppressed the steroidogenesis in Leydig cells (Fig. 1).…”

Section: Discussionmentioning

confidence: 89%

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Curcumin downregulates 8-br-cAMP-induced steroidogenesis in mouse Leydig cells by suppressing the expression of Cyp11a1 and StAR independently of the PKA-CREB pathway

et al. 2018

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Abstract. Although curcumin was widely applied as a functional food for different diseases, it was found to reduce serum testosterone level and fertility in male animals by unknown molecular mechanisms. Here in our study, we investigated the possible mechanisms of curcumin-suppressed testosterone production in Leydig cells. Our enzyme immunoassay results showed that curcumin cell-autonomously suppressed ovine luteinizing hormone-stimulated testosterone production in primary Leydig cells and 8-bromo-cyclic adenosine monophosphate (8-br-cAMP)-induced progesterone production in MA-10 cells. Furthermore, our real-time PCR, Western blot, and 22R-OHC/pregnenolone supplementing experiment data demonstrated that curcumin suppressed 8-br-cAMP-induced steroidogenesis in Leydig cells by inhibiting the expression of StAR and Cyp11a1. Interestingly, our Western blot data showed that although curcumin suppressed PKA activity, it did not alter the 8-br-cAMPinduced phosphorylation of CREB. On the contrary, the real-time PCR results showed that curcumin suppressed 8-br-cAMPinduced expression of Nr5a1 and Fos, which are crucial for cAMP-stimulated StAR and Cyp11a1 expression in Leydig cells. Collectively, our data demonstrated that curcumin may suppress cAMP-induced steroidogenesis in mouse Leydig cells by down-regulating Nr5a1/Fos-controlled StAR and Cyp11a1 expression independently of the PKA-CREB signaling pathway.

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Section: Discussionmentioning

confidence: 89%

“…The same study also showed increased percentage of morphologically abnormal spermatozoa and reduced sperm count, as well as the serum testosterone level in mice [8]. Of note, an in vitro study had demonstrated that curcumin also cellautonomously inhibited the LH-stimulated testosterone production in rat Leydig cells [9].…”

mentioning

confidence: 72%

Curcumin downregulates 8-br-cAMP-induced steroidogenesis in mouse Leydig cells by suppressing the expression of Cyp11a1 and StAR independently of the PKA-CREB pathway

et al. 2018

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“…Thus, the reduction in litter sizes of female mice treated with curcumin-PEG during breeding and pregnancy may be due to curcumin toxicities toward sperm and embryos. Litter sizes also were negatively impacted when male mice were fed high concentrations of curcumin and then mated with untreated females [31]. Hazardous reproductive effects of curcumin/curcumin-PEG are transient/reversible [31, 32, present study].…”

Section: Discussionmentioning

confidence: 99%

Reproductive Effects of Pegylated Curcumin.

Murphy

Tang

Kirk

et al. 2011

Biology of Reproduction

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Curcumin, a polyphenol derived from the rhizome turmeric, has potential as an anticancer agent. We synthesized an amphipathic/surfactant pegylated curcumin (curcumin-PEG) designed for parenteral administration. Objectives of these investigations were to assess side-effects of a therapeutic regimen of curcumin-PEG in a preclinical model. Intraperitoneal (ip) tumor burdens were reduced in athymic female mice grafted with human SKOV-3 ovarian adenocarcinoma cells and injected intravenously (iv) with curcumin-PEG. There were no gross anatomical or histopathological effects detected in non-reproductive organs. Uteri (luminal fluid imbibition) and ovaries (decreased folliculogenesis) were affected by treatment. Curcumin-PEG ip hastened the onset of puberty in immature female mice. Live births were reduced in mature females housed with males and treated iv with curcumin-PEG; mating (vaginal plugs) was not affected. Accessory gland weights, testicular testosterone concentrations, and spermatogenesis were diminished in mature male mice following iv curcumin-PEG. Estrogenic/antiandrogenic and pregnancydisrupting effects of a water soluble/bioavailable curcumin were demonstrated.

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“…A reduction in sperm count suggests alterations in sperm maturation and sperm production [3] . In our study, a decrease in the sperm count of cauda epididymis following treatment with F. limonia fruit pulp extract may be due to inhibition of the spermatogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…As we know the entire available contraceptive in the market are not safe, mostly they are steroid in nature and they have more or little hazardous side effect. Attention has now been focused on indigenous plants for possible contraceptive effect [1][2][3]. The traditional knowledge on the medicinal use of plants should be assessed under laboratory conditions using appropriate biological assays to disclose if the traditional claims are evidence-supported.…”

Section: Introductionmentioning

confidence: 99%

Reversible antispermatogenic and antisteroidogenic activities of Feronia limonia fruit pulp in adult male rats

Dhanapal

Ratna

Sarathchandran

et al. 2012

Asian Pacific Journal of Tropical Biomedicine

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Reversible antifertility effect of aqueous rhizome extract of Curcuma longa L. in male laboratory mice

Cited by 62 publications

References 11 publications

Curcumin downregulates 8-br-cAMP-induced steroidogenesis in mouse Leydig cells by suppressing the expression of Cyp11a1 and StAR independently of the PKA-CREB pathway

Curcumin downregulates 8-br-cAMP-induced steroidogenesis in mouse Leydig cells by suppressing the expression of Cyp11a1 and StAR independently of the PKA-CREB pathway

Reproductive Effects of Pegylated Curcumin.

Reversible antispermatogenic and antisteroidogenic activities of Feronia limonia fruit pulp in adult male rats

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