Reversible alterations of the surface of ascites tumour cells induced by a surface-active substance, tween 60

Malenkov, A. G.; Bogatyreva, S. A.; Bozhkova, V. P.; Modjanova, E.A.; Vasiliev, J. M.

doi:10.1016/0014-4827(67)90356-4

Cited by 23 publications

(6 citation statements)

References 15 publications

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“…These effects of tween 80 are possibly due to the action of the detergent on the cell membrane. In fact it has been found that tween can increase the permeability of cells to a number of substances such as ions, dyes and even proteins (Hodes et al, 1960;Kay, 1965;Malenkov et al, 1967). However, in these studies relatively large concentrations (1 %) of the detergents were used, while the reduction of drug resistance may be obtained with 100-fold lower concentrations of tween 80.…”

Section: Reversible the Cells Did Not Become Adapted To The Sensitizmentioning

confidence: 99%

The effect of non‐ionic detergent tween 80 on colcemid‐resistant transformed mouse cells in vitro

Stavrovskaya¹,

Потапова²,

Rosenblat³

et al. 1975

Intl Journal of Cancer

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The effect of the non-ionic detergent tween 80 on one colcemid-resistant and one sensitive subline of mouse L cells has been studied. The colcemid-resistant subline was also resistant to colchicine and Vinca alkaloids. Tween 80 at concentrations of 0.01% (v/v) or higher increased the sensitivity of drug-resistant cells to the antimitotic effect of colcemid, colchicine and vinblastine. Tween 80 also potentiated the initial rate and the maximal level of the (3-H)-cholchicine uptake by both sensitive and resistant cells. However, the detergent at concentrations of up to 1% had no effect on (3-H)-colchicine binding by cell homogenates. Thus it appears that the effects of tween 80 were due to an increase in cell membrane permeability to the drugs. The effect was completely reversible. The cells did not become adapted to the sensitizing action of tween 80 even after prolonged incubation in medium containing the detergent. A considerable increase in the permeability to the drug was obtained with doses of tween 80 which were non-toxic and which had no effect on cell proliferation, morphology and locomotion. The increase in membrane permeability caused by tween 80 at these concentrations was selective, the membrane permeability to ions and to (3-H)-2-deowy-D-glucose being, in fact, unchanged.

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Section: Reversible the Cells Did Not Become Adapted To The Sensitizmentioning

confidence: 99%

The effect of non‐ionic detergent tween 80 on colcemid‐resistant transformed mouse cells in vitro

Stavrovskaya¹,

Потапова²,

Rosenblat³

et al. 1975

Intl Journal of Cancer

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“…This is likely to be a molecular rearrangement or a phase transition within the phospholipid matrix of ER membrane, for example from a lamellar to a micellar distribution, which would account for a less efficient permeability barrier . Lamellar-micellar transitions, such as those described in model systems (Luzzati et al ., 1966), have also been proposed to explain the effects of detergent on the permeability of plasma membranes from intact cells (Malenkov et al ., 1967 ;Hodes et al ., 1969) and to account for the effect of lysolecithin which causes leakage of Ca++ from sarcoplasmic reticulum vesicles (Agostini and Hasselbach, 1971) and, in several systems, promotes membrane fusion before lysis (Lucy, 1970 ;Poole et al ., 1970) . It is interesting to note that membrane openings were detected by electron microscopy only in vesicles treated with levels of detergent near the CMC, and therefore sufficient to partially release membrane phospholipids .…”

Section: Insets Tomentioning

confidence: 99%

Selective Release of Content From Microsomal Vesicles Without Membrane Disassembly

Kreibich

Debey

Sabatini

1973

The Journal of Cell Biology

214

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Rat liver rough microsomes treated with a series of desoxycholate (DOC) concentrations from 0.003 to 0.4% were analyzed by isopycnic sucrose density gradient centrifugation in media containing high or low salt concentrations. Tritium-labeled precursors administered in vivo were used as markers for ribosomes (orotic acid, 40 h), phospholipids (choline, 4 h), membrane proteins (leucine, 3 days), and completed secretory proteins of the vesicular cavity (leucine, 30 min). Within a narrow range of DOC concentrations (0.025–0.05%), the vesicular polypeptides were selectively released from the microsomes, while ribosomes, nascent polypeptides, and microsomal enzymes of the electron transport systems were unaffected. The detergent concentration which led to leakage of content was a function of the ionic strength and of the microsome concentration. At the lowest effective DOC concentration the microsomal membranes became reversibly permeable to macromoles as shown by changes in the density of the vesicles in Dextran gradients and by the extent of proteolysis by added proteases. Incubation of rough microsomes with proteases in the presence of 0.025% DOC also led to digestion of proteins from both faces of the microsomal membranes and to a lighter isopycnic density of the membrane vesicles.

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“…Tween 80 may affect other cell structures as well, since cardiolipins, which are mitochondria1 constituents were completely lost as a result of the treatment with Tween 80. In a very careful study, Malenkov et al (9) incubated Ehrlich and hepatoma 22 cells with 1% Tween 60 in neutral phosphate buffer for 30-60 min. Tween made the cells susceptible t o lysis by trypsin and they stained diffusely with neutral red instead of revealing the usual neutral red granules.…”

Section: Detergents: Tween 80 a N D Tween 60mentioning

confidence: 99%

“…In a very careful study, Malenkov et al (9) incubated Ehrlich and hepatoma 22 cells with 1% Tween 60 in neutral phosphate buffer for 30-60 min. Tween made the cells susceptible t o lysis by trypsin and they stained diffusely with neutral red instead of revealing the usual neutral red granules.…”

Section: Oo:jssmentioning

confidence: 99%

Methods for rapidly altering the permeability of mammalian cells

Heppel

Makan

1977

J Supramol Struct

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Various agents alter mammalian cells so that they rapidly become nonspecifically permeable to substances that ordinarily do not penetrate intact cells. Thus, toluene renders liver cells permeable to nucleotides and macromolecules. Tween 80 and Tween 60 act on similar fashion, and the effect is reversible. Dextran sulfate reversibly alters the permeability of Ehrlich ascites tumor cells, which offers a tool for studying the control of macromolecular syntheses and other processes. Brief exposure to external ATP alters the permeability of certain transformed mouse cells but not of untransformed cells. The effect of ATP is rapidly reversible.

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Reversible alterations of the surface of ascites tumour cells induced by a surface-active substance, tween 60

Cited by 23 publications

References 15 publications

The effect of non‐ionic detergent tween 80 on colcemid‐resistant transformed mouse cells in vitro

The effect of non‐ionic detergent tween 80 on colcemid‐resistant transformed mouse cells in vitro

Selective Release of Content From Microsomal Vesicles Without Membrane Disassembly

Methods for rapidly altering the permeability of mammalian cells

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