Reversed-phase pH gradient thin-layer chromatography of biologically active substances with controlled developing solvent velocity

Rapid analysis of differential chemical compositions of Poria cocos using thin-layer chromatography spray ionization-mass spectrometry

“…In recent years, the development of analytical methods has led to the introduction of ambient ionization-mass spectrometry (AIMS) into drug analysis, which is appreciated for its high selectivity, ease of automation and intelligence, as well as its ability for rapid analysis in situ, 13,14 such as electrospray-assisted laser desorption ionization (ELDI), 15 desorption electrospray ionization (DESI), 16,17 direct analysis in real-time (DART), [18][19][20] or matrix-assisted laser desorption/ionization (MALDI). 21,22 In addition, in 2003, Shiea et al cut a TLC plate end as a tip shape and dropped the sample to the tip, and the principle of electrospray was applied to MS analysis.…”

“…12 By dipping unknown compounds on the silica gel plate, TLC will be able to compare the R f value of the spots to characterize the compounds. 13 In contrast, TLC analysis only provides information on the known spots, and the information on compounds in unknown spots needs to be combined with other analytical tools.…”