Reversed-phase liquid chromatographic determination of isoniazide in human urine as a test of the genetically predetermined type of biotransformation by acetylation

Evgen'ev, M. I.; Garmonov, S. Yu.; Evgen'eva, I. I.; Goryunova, S. M.; Погорельцев, В. И.; Levinson, F. S.

doi:10.1016/s0039-9140(98)00166-0

Cited by 17 publications

(4 citation statements)

References 6 publications

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“…7, sample 11 is the lab control which contains only N‐M bead test reagents. The N‐M bead test could identify sample 4 as the only negative sample and sample 2 as the low titer (< 1 μg/ml) INH sample (determined by HPLC [41, 42]). The rest of the samples has INH levels ranging from 2 to 10 μg/ml and the N‐M bead test gave positive for the same.…”

Section: Resultsmentioning

confidence: 99%

Objective measurement of isoniazid levels: practical approach for monitoring tuberculosis drug treatment adherence

Korrapati

Muneppa

Pullela

et al. 2017

IET nanobiotechnol.

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Drug treatment adherence is one of the biggest issues in tuberculosis (TB) treatment. Including DOTS (Directly Observed Treatment, Short-course) program, many programs for TB cases have yielded marginal success and the new drug resistance varieties of TB persist. During the 1960s, researchers have projected naphthoquinone-mercuric chloride (N-M) test as the single most way to objectively track the presence of isoniazid (INH) in urine and hence treatment adherence. INH is metabolised by cytochrome P450s and usually cleared from body within 8 h. Hence, the typical INH levels in biological fluids are within 1-5 μg/ml. The N-M test practically works at 10-50 μg/ml and the authors developed the sodium polyacrylate (SPA) as a solid surface to improve detection limit of INH by 6-10 fold. The modification involves absorbing the reagents of N-M test on the SPA surface and lyophilised material is directly used for diagnosis. The dry beads can detect 1-2 μg/ml of INH in saliva. The test performed with spiked as well as clinical samples and the results have good correlation. This new test has a realistic chance of tracking the TB treatment adherence remotely and in that direction a system was projected for implementation.

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Section: Resultsmentioning

confidence: 99%

Objective measurement of isoniazid levels: practical approach for monitoring tuberculosis drug treatment adherence

Korrapati

Muneppa

Pullela

et al. 2017

IET nanobiotechnol.

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“…The most wide-scale and influential of these is the chemical pollution of the environment by xenogenic artificial chemical substances. Additional factors include: toxic chemical elements, organic pesticides and other substances of industrial and household genesis [1][2][3]. These pollutants remain in the ecological nutritional chains for an extended period of time, which leads to a rise in a number of acute and chronic diseases (cancer, Alzheimer's disease, Parkinson's disease) [4,5].…”

Section: Introductionmentioning

confidence: 99%

“…With respect to ecology, determining biochemical phenotypes of toxicants metabolism of industrial personnel and other people is necessary for the assessment of risks of intoxication, oncogenesis, individual chemical sensitivity, occupational fitness, uncovering groups of heightened risk and to lessen the development of unfavorable ecological factors. The impact of ecological factors may lead to an increase in the inherited numbers of pathologies, which are the root of many different ailments related to metabolic disorders [2,6].…”

Section: Introductionmentioning

confidence: 99%

Biomonitoring of organic and inorganic chemicals in the hair of Vietnamese people via spectral and chromatographic analysis

Cuong¹,

Thuy²,

Evgen'ev³

2013

JBPC

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A combination of spectral and chromatographic analytical methods has been executed to monitor chemicals in the hair of Vietnamese people. Methods were developed for the determination of some chemical elements in hair by inductively coupled-plasma atomic emission spectroscopy with limits of detection reaching 1 - 100 μg·l^－¹. A supercritical fluid extraction (SFE) and clean-up procedures were established for isolating organochlorine pollutants (OCPs) and organophosphorus pesticides (OPPs) from the hair of the Vietnamese test subjects. Eight OCPs and OPPs were determined by SFE with carbon dioxide only and methanol-modified carbon dioxide extraction at 350 bar and 45℃, followed by gas chromatography coupled to mass spectrometry. The results indicated that OCPs can be extracted quantitatively using CO₂ only, whereas OPPs require a modifier for extraction. The limits of detection that were found were between 0.7 to 1.3 μg·g^－¹, and good linearity (r² > 0.9861) was achieved within the tested ranges. The activities of the N-acetyltransferase (NAT) and the monooxygenases cytochrome P450 (CYP 450) were determined and compared to the acetylation and oxidation phenotypes of healthy Vietnamese test subjects. The variations in the concentration of chemical elements in human hair were shown to be dependent upon the activities of the two-phase metabolic system, on the individual’s gender and the duration of residence within Russia.

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“…A number of analytical methods are described in the literature including brom o m e t ry (5), nonaqueous titrimetry (6), spectro p h o t o m e t ry (7)(8)(9), chemiluminescence (10), fluorimetry (11)(12)(13), colorimetry (14,15), gas chromatography (GC) (16), and GC-mass spectro me t ry (17). Since their introduction, high-perf o rmance liquid c h romatography (HPLC) methods have become more used (18)(19)(20)(21)(22)(23)(24)(25)(26). We developed a high-perf o rmance thin-layer chromatography (HPTLC) of INH and AcINH with liquid-liquid extraction (27).…”

Section: Introductionmentioning

confidence: 99%

Solid-Phase Extraction and HPTLC Determination of Isoniazid and Acetylisoniazid in Serum. Comparison with HPLC

Guermouche¹,

Guermouche²

2004

Journal of Chromatographic Science

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A new solid-phase extraction (SPE) and quantitative determination of isoniazid (INH) and its acetyl metabolite (AcINH) in serum by high-performance thin-layer chromatography (HPTLC) is presented. Alkalized serum samples with nicotinamide as an internal standard are applied to an SPE cartridge containing a new SPE sorbent, [poly (divinylbenzene-co-N-vinylpyrrolidone )]. A simple procedure of conditioning, washing, and eluting steps is described. After evaporation of the eluates to dryness and reconstitution, one-dimensional HPTLC is performed on silica gel plates with ethyl acetate-methanol (70:30) as a mobile phase. Quantitation is done by densitometry. Convenient validation parameters are obtained (linearity, limits of detection and quantitation, precision, and accuracy) for INH and AcINH. The method is compared with a high-performance liquid chromatographic (HPLC) technique developed in the laboratory, and satisfactory correlation is found between data from the two techniques. The HPTLC method is sensitive and specific and is used to quantitate INH and AcINH in patient blood serum, and the results are compared with those obtained by HPLC.

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Reversed-phase liquid chromatographic determination of isoniazide in human urine as a test of the genetically predetermined type of biotransformation by acetylation

Cited by 17 publications

References 6 publications

Objective measurement of isoniazid levels: practical approach for monitoring tuberculosis drug treatment adherence

Objective measurement of isoniazid levels: practical approach for monitoring tuberculosis drug treatment adherence

Biomonitoring of organic and inorganic chemicals in the hair of Vietnamese people via spectral and chromatographic analysis

Solid-Phase Extraction and HPTLC Determination of Isoniazid and Acetylisoniazid in Serum. Comparison with HPLC

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