Reversed-phase ion-pair liquid chromatographic procedure with electrochemical detection for the analysis of urinary thiosulphate

K»gedal, Bertil; Källberg, Magnus; M»rtensson, Johannes; Sörbo, Bo

doi:10.1016/s0378-4347(00)84412-6

Cited by 25 publications

(7 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thiosulfate was clearly detected in the urine samples from workers A, B and C at concentrations of 0.43, 0.39 and 0.12 µmol/ml, respectively. These values were 4-14 times higher than 0.03 µmol/ml in healthy persons [14][15][16]. These results are in agreement with the results of our animal experiments.…”

Section: Resultssupporting

confidence: 92%

The usefulness of thiosulfate as an indicator of hydrogen sulfide poisoning: three cases

Kage¹,

Takekawa²,

Kurosaki

et al. 1997

International Journal of Legal Medicine

View full text Add to dashboard Cite

We examined the usefulness of thiosulfate as an indicator of hydrogen sulfide poisoning by analysing sulfide and thiosulfate in three cases. In the first (non-fatal) case sulfide and thiosulfate were not detected in the blood samples from any of the four workers involved in the accident. In the urine samples, only thiosulfate was detected in three out of the four workers at a concentration of 0.12-0.43 micromol/ml, which was 4-14 times higher than the level in a healthy person. In the second (fatal) case sulfide and thiosulfate were detected in the blood sample at concentrations of 0.007 micromol/ml for sulfide, and 0.025 micromol/ml for thiosulfate. The thiosulfate concentration was at least 8 times higher than the level in a healthy person. In the third (fatal) case sulfide and thiosulfate were detected in the blood sample at concentrations of 0.95 micromol/ml for sulfide, and 0.12 micromol/ml for thiosulfate. Based on the above results, we concluded that thiosulfate in urine is the only indicator to prove hydrogen sulfide poisoning in non-fatal cases, while the analysis of sulfide in fatal cases should be accompanied by the measurement of thiosulfate in blood.

show abstract

Section: Resultssupporting

confidence: 92%

The usefulness of thiosulfate as an indicator of hydrogen sulfide poisoning: three cases

Kage¹,

Takekawa²,

Kurosaki

et al. 1997

International Journal of Legal Medicine

View full text Add to dashboard Cite

show abstract

“…The excretion values of all compounds were based on two 24-h urine samples, identical to the procedure of the patients. The inter-assay coefficient of variation (CV) was less than 8% for all compounds, as shown previously (11,(17)(18)(19)(20)(21).…”

Section: '1supporting

confidence: 80%

“…Sulfur amino acids were determined by conventional amino acid analysis (11); mercaptolactate, mercaptoacetate, and N-acetylcysteine by gas chromatography (1 8); thiocyanate spectrophotometrically (19,20); and thiosulfate by liquid chromatography (21). Statistical analysis was performed with the two-tailed Students' t test; differences obtained between patient and control groups were regarded as significant when p < 0.05.…”

Section: Methodsmentioning

confidence: 99%

Sulfur Amino Acid Metabolism in Hepatobiliary Disorders

Mårtensson¹,

Foberg²,

Frydén

et al. 1992

Scandinavian Journal of Gastroenterology

View full text Add to dashboard Cite

Sulfur amino acid metabolism was studied in patients with mild to severe forms of liver dysfunction and compared with that of healthy controls. Patients with mild liver dysfunction (for example, Gilbert's syndrome) had a normal sulfur amino acid metabolism. With increased inflammatory activity and cirrhosis (for example, chronic active hepatitis, alcohol-induced cirrhosis, and hepatic coma) a decreased ability to metabolize methionine (to cysteine, with cystathionine accumulation) and cysteine (to inorganic sulfate, with thiosulfate and N-acetylcysteine accumulation) was found. In contrast, transaminative metabolism of sulfur amino acids was preserved in patients with advanced forms of liver dysfunction, suggesting that transamination of sulfur amino acids is performed not only in the liver but also in extrahepatic tissues. Some implications of these findings are discussed.

show abstract

“…3,14 However thiosulfate is naturally present in very low levels in blood and urine even in healthy individuals. [15][16][17] In both cases, the blood levels of thiosulfate was above the toxic level 1,2,5,18,19 and was sufficient to cause death.…”

Section: Discussionmentioning

confidence: 99%

Hydrogen sulfide toxicity: two case reports

Munasinghe¹,

Rascon²,

Diaze³

et al. 2018

Sri Lanka J Forensic Med, Sci & Law

View full text Add to dashboard Cite

Hydrogen sulfide (H2S) toxicity is a known occupational/industrial hazard in organic decaying disposal, sulfur springs, natural gas well and coal pits. We report two deaths due to acute accidental hydrogen sulfide poisoning. Two males 40 and 36 years old were found dead in two different water treatment plants after spilling of sewage. Autopsy revealed greenish discoloration of skin and brain in one individual and pulmonary oedema in both. Toxicology revealed lethal levels of thiosulfate which is a metabolite of H2S. Death investigation concluded that both fatalities were due to toxicity of H2S in an accidental manner. Toxicological investigations are important in cases where occupational hazards are suspected.

show abstract

Reversed-phase ion-pair liquid chromatographic procedure with electrochemical detection for the analysis of urinary thiosulphate

Cited by 25 publications

References 17 publications

The usefulness of thiosulfate as an indicator of hydrogen sulfide poisoning: three cases

The usefulness of thiosulfate as an indicator of hydrogen sulfide poisoning: three cases

Sulfur Amino Acid Metabolism in Hepatobiliary Disorders

Hydrogen sulfide toxicity: two case reports

Contact Info

Product

Resources

About