Reversed-phase high-performance liquid chromatographic procedure for the determination of maysin in corn silks

Snook, M. E.; Widstrom, N. W.; Gueldner, R. C.

doi:10.1016/s0021-9673(01)89654-0

Cited by 48 publications

(61 citation statements)

References 9 publications

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“…The R2R3-MYB P1 transcription factor regulates maysin production in silk tissues of some maize varieties (37)(38)(39). Our results show that UGT708A6, the expression of which is regulated by P1 in silks (25), generates isovitexin and isoorientin, intermediates involved in biosynthesis of apimaysin and maysin, respectively (16), suggesting that this enzyme could be involved in this biosynthetic pathway.…”

Section: Discussionmentioning

confidence: 74%

“…First, we evaluated how genes of candidates were expressed in different maize tissues and whether they are regulated by the P1 transcription factor, extensively known to be involved in the regulation of C-glycosyl flavone biosynthesis (16,(37)(38)(39). Therefore, we built a list of 157 putative UGTs in maize using bronze1 (GRMZM2G165390), one of the best studied maize UGTs and one involved in anthocyanin biosynthesis (40,41), as a starting point.…”

Section: Resultsmentioning

confidence: 99%

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Identification of a Bifunctional Maize C- and O-Glucosyltransferase

Ferreyra

Rodrı́guez

Casas

et al. 2013

Journal of Biological Chemistry

128

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Section: Discussionmentioning

confidence: 74%

Section: Resultsmentioning

confidence: 99%

Identification of a Bifunctional Maize C- and O-Glucosyltransferase

Ferreyra

Rodrı́guez

Casas

et al. 2013

Journal of Biological Chemistry

128

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“…While still frozen, each silk sample was placed in a glass jar with 120 ml methanol and stored at 0°C for 14 days. Extract concentrations of maysin were determined by reversed-phase HPLC (26,27) and expressed as percent fresh silk weight.…”

Section: Methodsmentioning

confidence: 99%

Quantitative trait loci and metabolic pathways: genetic control of the concentration of maysin, a corn earworm resistance factor, in maize silks.

Byrne

McMullen²,

Snook³

et al. 1996

Proc. Natl. Acad. Sci. U.S.A.

186

139

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Interpretation of quantitative trait locus (QTL) studies of agronomic traits is limited by lack of knowledge of biochemical pathways leading to trait expression. To more fully elucidate the biological significance of detected QTL, we chose a trait that is the product of a well-characterized pathway, namely the concentration of maysin, a C-glycosyl flavone, in silks of maize, Zea mays L. Maysin is a host-plant resistance factor against the corn earworm, Helicoverpa zea (Boddie). We determined silk maysin concentrations and restriction fragment length polymorphism genotypes at flavonoid pathway loci or linked markers for 285 F2 plants derived from the cross of lines GT114 and GT119.Single-factor analysis of variance indicated that the p1 region on chromosome 1 accounted for 58.0%o of the phenotypic variance and showed additive gene action. The pi locus is a transcription activator for portions of the flavonoid pathway. A second QTL, represented by marker umclO5a near the brown pericarpi locus on chromosome 9, accounted for 10.8% of the variance. Gene action of this region was dominant for low maysin, but was only expressed in the presence of a functional pi allele. The model explaining the greatest proportion of phenotypic variance (75.9%) included pi, umclOSa, umcl66b (chromosome 1), ri (chromosome 10), and two epistatic interaction terms, pi x umclO5a and pi x ri. Our results provide evidence that regulatory loci have a central role and that there is a complex interplay among different branches of the flavonoid pathway in the expression of this trait.Development of molecular-marker linkage maps in many species facilitates the identification of chromosome regions associated with variation in quantitative traits (1). By dissecting the continuous phenotypic variation typical of many traits into contributions from discrete genetic factors, quantitative trait locus (QTL) studies provide insights into trait inheritance and genome organization, and often are sufficient to initiate marker-assisted selection. However, the biological interpretation of QTL data is generally limited by lack of knowledge of the genetics, biochemistry, and physiology underlying trait expression. To advance the level of QTL interpretation, we analyzed variation in an economically important trait that is determined by a well-characterized genetic and biochemical pathway.The corn earworm (CEW) is a major silk-and kernelfeeding insect pest of maize in the United States and parts of Latin America (2, 3). Host-plant resistance to CEW results from both antibiosis due to chemical factors in silks (stylar/ stigmatic tissue), and morphological features such as tight covering of the ear by husk leaves (4). Understanding of the nature of antibiosis to CEW was advanced when maysin (Fig. 1), a C-glycosyl flavone that inhibits CEW larval growth, was isolated from silks of the Mexican maize landrace "Zapalote Chico" (5). Later, Wiseman et al. (6) found a highly significant relationship between increased silk maysin concentration and reduced earworm ...

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“…The luteolin C-glycoside maysin was reported as an antifeedant compound toxic to the corn earworm Heliothis zea by Elliger et al (1980). An HPLC methodology for the detection of this compound in corn silk was published by Snook et al (1989). The C-4Љ-hydroxy derivatives of maysin and 3Ј-methoxymaysin from corn silks (Zea mays) were reported by Snook et al (1995).…”

Section: Phenolic and Flavonoid Content Free Radical Scavenging Effementioning

confidence: 99%

Free Radical Scavengers from Cymbopogon citratus (DC.) Stapf Plants Cultivated in Bioreactors by the Temporary Immersion (TIS) Principle

Tapia

Cheel

Theoduloz

et al. 2007

Zeitschrift Für Naturforschung C

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The biomass production of Cymbopogon citratus shoots cultivated in bioreactors according to the temporary immersion (TIS) principle was assessed under different growth conditions. The effect of gassing with CO 2 -enriched air, reduced immersion frequency, vessel size and culture time on total phenolic and flavonoid content and free radical scavenging effect of the methanolic extracts was measured. From the TIS-culture of C. citratus, seven compounds were isolated and identified as caffeic acid (1), chlorogenic acid (2), neochlorogenic acid (3), p-hydroxybenzoic acid (4), p-hydroxybenzoic acid 3-O--d-glucoside (5), glutamic acid (6) and luteolin 6-C-fucopyranoside (7). The occurrence of compounds 1Ð7 and their variability in C. citratus grown under different TIS conditions was determined by HPLC. The free radical scavenging effect of the methanolic extract and compounds was measured by the discoloration of the free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH). The main metabolites in 6-and 8-week-old cultures, both in 5 and 10 l vessels, were chlorogenic acid (2) (100Ð113 mg%) and neochlorogenic acid (3) (80Ð 119 mg%), while in the cultures with CO 2 -enriched air and reduced immersion frequency the main compound detected in the extracts was glutamic acid (6) (400 and 670 mg% for the green and white biomass and 619 and 630 mg% for the green and white biomass, respectively). The most active compounds, as free radical scavengers, in the DPPH discoloration assay were caffeic acid (1), chlorogenic acid (2), neochlorogenic acid (3) and the flavonoid luteolin 6-C-fucopyranoside (7).

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Reversed-phase high-performance liquid chromatographic procedure for the determination of maysin in corn silks

Cited by 48 publications

References 9 publications

Identification of a Bifunctional Maize C- and O-Glucosyltransferase

Identification of a Bifunctional Maize C- and O-Glucosyltransferase

Quantitative trait loci and metabolic pathways: genetic control of the concentration of maysin, a corn earworm resistance factor, in maize silks.

Free Radical Scavengers from Cymbopogon citratus (DC.) Stapf Plants Cultivated in Bioreactors by the Temporary Immersion (TIS) Principle

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