Reversed-Phase High-Performance Liquid Chromatographic Determination of Mitomycin C in Human Serum

Buice, Robert G.; Sidhu, Paramjeet; Gurley, Bill J.; Niell, Harvey B.

doi:10.1097/00007691-198403000-00018

Cited by 17 publications

(3 citation statements)

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“…MMC was determined in aqueous humour by a modi fication of the HPLC method of Buice et al 18 A solution of the internal standard, p-aminoacetophenone, 5 j..! g/ml was added to 100 j..!…”

Section: Hplc Methodfor Determination Of MMC In Aqueous Humour18-21mentioning

confidence: 99%

Mitomycin-C concentration in human aqueous humour following trabeculectomy

Seah

Prata

Minckler

et al. 1993

Eye

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The aim of the study was to determine mitomycin-C (MMC) concentrations in human aqueous humour during trabeculectomy and to correlate anterior chamber concentrations with method of application. MMC was applied intra-operatively by saturating sponges, ranging in size from 2 x 2 x 5 mm to 2 x 4 x 10 mm on dry cut, with 0.5 mg/ml MMC during trabeculectomy for 3-5 minutes. Applications to episclera were made in 18 cases and to the scleral bed after scleral flap dissection in 9 cases. Aqueous samples were collected by paracentesis with a 30 gauge needle 2-7 minutes after removal of sponge and external irrigation. Aqueous MMC concentrations were determined by high-performance liquid chromatography. Aqueous MMC concentration in 27 samples ranged from below minimum detectable concentration (less than 5 ng/ml) to 120.8 ng/ml. Mean aqueous drug levels obtained when the applications were to the scleral bed were 35.65 +/- 39.17 ng/ml (range 5-120.8 ng/ml). Applications on episclera gave mean aqueous concentrations of 4.98 +/- 9.11 ng/ml (range 0-33.3 ng/ml). The difference was statistically significant (p = 0.004). There were no correlations between sponge size, time of MMC exposure and aqueous MMC level. In conclusion, MMC is detectable in aqueous humour within minutes of external application and the aqueous concentration level is higher if the application is in the scleral bed than on the episclera. Toxicity of the drug at this concentration range for corneal endothelial cells needs further investigation via in vitro and clinical studies.

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“…MMC was determined in aqueous humour by a modi fication of the HPLC method of Buice et al 18 A solution of the internal standard, p-aminoacetophenone, 5 j..! g/ml was added to 100 j..!…”

Section: Hplc Methodfor Determination Of MMC In Aqueous Humour18-21mentioning

confidence: 99%

Mitomycin-C concentration in human aqueous humour following trabeculectomy

Seah

Prata

Minckler

et al. 1993

Eye

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“…Chemical structure of mitomycin C. [4,5], others on HPLC [6][7][8][9][10][11][12][13][14][15][16][17][18][19][20]. One of these reported a sensitive and specific HPLC method for mitomycin C in biological fluids which requires extensive sample preparation and liquid-liquid extraction [6].…”

Section: Introductionmentioning

confidence: 99%

“…One of these reported a sensitive and specific HPLC method for mitomycin C in biological fluids which requires extensive sample preparation and liquid-liquid extraction [6]. Other authors [9][10][11] used a solid-phase extraction procedure employing Amberlite XAD-2 resin [7] or Sep-Pak Cls cartridges [8][9][10] or Porapak Q [11] in order to extract the drug from biological fluids. A direct injection HPLC method is described for the determination ofmitomycin C in human plasma [12] and in human aqueous humor and serum [ 13].…”

Section: Introductionmentioning

confidence: 99%

Mitomycin C in biological fluids: Comparison of disk and cartridge solid-phase extraction and quantitation by HPLC in human plasma and ultrafiltrate

et al. 1999

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KeyWordsColumn liquid chromatography Mitomycin C Disk and SPE cartridges Human plasma ultrafiltrate SummaryThe performance of particle-loaded disks and traditional, packed extraction columns is compared. In this study, solid-phase extraction and liquid chromatographic analysis of the anticancer drug mitomycin C are duplicated using two devices that contain chemically similar Cxs bonded silica. The following parameters are used to assess performance: linearity, recovery, precision, and lowest limit of detection. The extraction of mitomycin C from plasma and ultrafiltrate using particle-loaded membranes achieves better precision, improved concentration ability, lower detection limits, and greater efficiency. In addiction, elution of drug from the membrane adsorbent is efficiently accomplished using a small volume of mobile phase, eliminating analyte instability problems and the need for additional timeconsuming evaporation.

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Pharmacokinetics of mitomycin C in non-oat cell carcinoma of the lung

Buice

Niell

Sidhu

et al. 1984

Cancer Chemother. Pharmacol.

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The disposition kinetics of the cancer chemotherapeutic agent mitomycin C have been studied in six male patients receiving mitomycin C in combination with cisplatin and vinblastine for non-oat cell carcinoma of the lung. Following rapid IV administration of mitomycin C (10 mg/m2), serum concentration-time course data were biexponential, with biologic half-lives of 46.2 +/- 12.1 min (mean +/- SD). Pharmacokinetic analysis of data by the CSTRIP and NONLIN digital computer programs generated parameters which suggested extensive distribution (V area = 656.8 +/- 169.8 ml X kg-1, mean +/- SD) and, as reported for other alkylating agents, rapid elimination (total body clearance = 10.3 +/- 3.2 ml X kg-1 X min-1, mean +/- SD). Interpatient variations in pharmacokinetic parameters were relatively small, suggesting that close monitoring of mitomycin C therapy might be unnecessary in patients with normal renal and hepatic function.

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Reversed-Phase High-Performance Liquid Chromatographic Determination of Mitomycin C in Human Serum

Cited by 17 publications

References 0 publications

Mitomycin-C concentration in human aqueous humour following trabeculectomy

Mitomycin-C concentration in human aqueous humour following trabeculectomy

Mitomycin C in biological fluids: Comparison of disk and cartridge solid-phase extraction and quantitation by HPLC in human plasma and ultrafiltrate

Pharmacokinetics of mitomycin C in non-oat cell carcinoma of the lung

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