Reversed-phase electrochromatography of amino acids and peptides using porous polymer monoliths

Shediac, Renée; Ngola, Sarah M.; Throckmorton, Daniel J.; Anex, Deon S.; Shepodd, Timothy J.; Singh, Anup K.

doi:10.1016/s0021-9673(01)01036-6

Cited by 112 publications

(68 citation statements)

References 39 publications

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“…While numerous monolithic structures have been fabricated using sol-gel technology [10][11][12][13][14][15] or particle immobilization with the aid of monolithic phases [16], the organic polymeric monoliths have proven to be more appropriate for the analysis of larger molecular weight, biologically relevant compounds such as peptides, proteins, and DNA [17][18][19][20][21][22]. The polymeric monoliths are readily fabricated by a copolymerization process of a mixture of monomers in the presence of a porogenic solvent.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic device for capillary electrochromatography‐mass spectrometry

Lazar

Yang

et al. 2003

Electrophoresis

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A novel microfabricated device that integrates a monolithic polymeric separation channel, an injector, and an interface for electrospray ionization-mass spectrometry detection (ESI-MS) was devised. Microfluidic propulsion was accomplished using electrically driven fluid flows. The methacrylate-based monolithic separation medium was prepared by photopolymerization and had a positively derivatized surface to ensure electroosmotic flow (EOF) generation for separation of analytes in a capillary electrochromatography (CEC) format. The injector operation was optimized to perform under conditions of nonuniform EOF within the microfluidic channels. The ESI interface allowed hours of stable operation at the flow rates generated by the monolithic column. The dimensions of one processing line were sufficiently small to enable the integration of 4-8 channel multiplexed structures on a single substrate. Standard protein digests were utilized to evaluate the performance of this microfluidic chip. Low- or sub-fmol amounts were injected and detected with this arrangement.

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Section: Introductionmentioning

confidence: 99%

Microfluidic device for capillary electrochromatography‐mass spectrometry

Lazar

Yang

et al. 2003

Electrophoresis

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“…Dodecyl acrylate, stearyl methacrylate, and poly(-oxyethylene) are used as retentive ligands with charged monomers, such as acrylic acid, vinyl sulfonic acid, 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS), and dimethyl diallylammonium chloride [45][46][47][48]. Acrylicbased hydrophobic monolithic stationary phases can be polymerized via free radical formation (using ammonium persulfate and TEMED) or photoinitiation [49]. Recently, novel cationic monoliths, based on the copolymerization of pentaerythritol diacrylate monostearate (PEDAS) with four different quaternary amine acrylic monomers, have been introduced for CEC [50], which is apparently an excellent separation bed to achieve strong EOF.…”

Section: One-step Preparation Of Charged Monolithsmentioning

confidence: 99%

Theoretical and nomenclatural considerations of capillary electrochromatography with monolithic stationary phases

Végvári

Guttman

2006

Electrophoresis

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During the past decade, CEC has been one of the few novel achievements in the field of separation science attracting a wide interest. The technology progress permitted the realization of the long-sought idea to employ an electroosmotically driven flow through the columns improving the separations in terms of both resolution and efficiency. The early practical obstacles related to the use of conventional bead-packed columns have been solved by the introduction of continuous beds, also known as monoliths. Hitherto, various synthesis approaches have been successfully developed producing monolithic beds in situ in capillary columns, sharing similar physical structure built up of tiny particles (in the sub-microm range) that are covalently linked together and to the capillary wall. Parallel with the practical column technology studies, the theory of electrochromatography has been continuously developed, focusing on such basic issues as EOF characterization, separation efficiency, and peak dispersion effects. This review provides a short introduction to the theory of CEC with special attention to monolithic separation beds. The paper also summarizes the latest achievements in CEC and discusses the nomenclature, EOF characteristics, and some specific advantages of monolithic column technology.

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“…In general, there are two types of monolithic columns which have been developed for chromatography: silica-based monolithic column [22][23][24] and organic polymer-based monolithic column [25,26]. Silica-based monolithic columns are produced by using the sol-gel approach to make a continuous sol-gel network throughout the column.…”

Section: Introductionmentioning

confidence: 99%

Separation of Inorganic Anions Using Methacrylate-Based Monolithic Column Modified with Trimethylamine in Ion Chromatography Capillary System

Mairizki¹,

Rahmah²,

nbsp³

et al. 2013

AJAC

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Methacrylate-based monolithic column was prepared in fused-silica capillary (80  0.32 mm i.d.) by in situ polymerizetion reaction using glycidyl methacrylate as monomer; ethylene dimethacrylate as crosslinker; 1-propanol, 1,4-butanediol, and water as porogenic solvents. The monolith matrix was modified with trimethylamine to create strong anion exchanger via ring opening reaction of epoxy groups. The morphology of the monolithic column was studied by using Scanning Electron Microscope (SEM). This column had good mechanical stability and permeability. The effects of various mobile phases for separation of inorganic anions were investigated. Iodate, bromate, nitrite, bromide, and nitrate were separated within 11 min using 100 mM potassium chloride as mobile phase and detected at 210 nm. This method showed good precision of retention time, acceptable linearity and good sensitivity. Under the optimum condition, the RSD of the retention time was in the range of 1.09% -1.75% (n = 6). The calibration curve showed linear relationships between the peak area and the concentration. The limits of detection (LOD) and the limits of quantitation (LOQ) were between 0.08 -0.18 mM and 0.26 -0.61 mM, respectively. This method was applied to the determination of inorganic anions in tap water and ground water samples.

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Reversed-phase electrochromatography of amino acids and peptides using porous polymer monoliths

Cited by 112 publications

References 39 publications

Microfluidic device for capillary electrochromatography‐mass spectrometry

Microfluidic device for capillary electrochromatography‐mass spectrometry

Theoretical and nomenclatural considerations of capillary electrochromatography with monolithic stationary phases

Separation of Inorganic Anions Using Methacrylate-Based Monolithic Column Modified with Trimethylamine in Ion Chromatography Capillary System

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