Reversed-phase chiral HPLC and LC/MS analysis with tris(chloromethylphenylcarbamate) derivatives of cellulose and amylose as chiral stationary phases

Peng, Liming; Jayapalan, Swapna; Chankvetadze, Bezhan; Farkas, Tamás

doi:10.1016/j.chroma.2010.08.075

Cited by 124 publications

(67 citation statements)

References 40 publications

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“…Because the interactions between analytes and selectors, responsible for enantiorecognition, are not completely understood and mainly based on hypotheses [2][3][4], it is hardly possible to predict experimental conditions which will ensure the separation of enantiomers. Consequently in the past many efforts were put into the development of generic (screening) strategies for chiral compounds [5][6][7][8][9][10][11][12][13][14][15]. The goal of these strategies is to have a set of experimental conditions that allow separating a broad range of chiral substances into their enantiomers in a limited number of experiments.…”

Section: Introductionmentioning

confidence: 99%

Test-set reduction in the screening step definition of a chiral separation strategy in polar organic solvents chromatography

Ates

Desmedt

Heyden

2012

Journal of Chromatography B

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Section: Introductionmentioning

confidence: 99%

Test-set reduction in the screening step definition of a chiral separation strategy in polar organic solvents chromatography

Ates

Desmedt

Heyden

2012

Journal of Chromatography B

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“…Also few methods have been reported for the resolution of doxylamine enantiomers in human plasma by using capillary electrophoresis 10 and high performance liquid chromatography. 11,12 However the reported methods have their own drawbacks like individual enantiomers (d)-and (l)-doxylamine were not identified, the methods were developed in the normal phase mode and also the stability of the enantiomers was not determined.…”

Section: Introductionmentioning

confidence: 98%

Chiral separation of (d)- and (l)-enantiomers of doxylamine succinate in rat plasma

Sirisha

Gurupadayya

Inturi

2015

Bulletin of Faculty of Pharmacy, Cairo University

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A selective chiral ultra fast liquid chromatography (UFLC-DAD) method was developed and validated to separate and quantify the (d)-and (l)-enantiomers of doxylamine in rat plasma. After extraction of the plasma samples with acetonitrile, the separation of doxylamine succinate enantiomers and internal standard (I.S., diphenhydramine hydrochloride) was achieved on a cellulose Tris (4-chloro,3-methylphenylcarbamate) column with a mobile phase of 20 mM ammonium bicarbonate buffer-acetonitrile (65:35 v/v) with 0.15% diethylamine in the buffer at a flow rate of 1.0 mL/min. The diode array (DAD) detection wavelength was set at 220 nm. The peaks obtained were identified as (d) and (l) by injecting the pure (d) form into the liquid chromatography and comparing the chromatograms. The effect of column oven temperature on the retention of doxylamine and mobile phase variables which have an effect on the enantiomers separation like ionic strength, type and concentration of organic modifier was studied. Linear calibration curves were obtained over the range of 100-1400 ng/mL in plasma for both enantiomers (R 2 > 0.995). The mean extraction recoveries were 94.5-104.7% of rat plasma. The mean relative standard deviation (RSD%) of accuracy and intra-day and inter-day precision for both enantiomers were 610%. The method can be further applied to determine the pharmacokinetics of (d)-and (l)-enantiomers in rat plasma.ª 2015 Production and hosting by Elsevier B.V. on behalf

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“…From the number of papers published over the past years, high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE) were two of the most commonly utilized techniques in chiral separation, of which HPLC is the dominant method [15]. To support drug metabolism and pharmacokinetic studies of chiral pharmaceuticals, it is necessary to combine the resolving power of HPLC with the sensitivity of MS detection [16]. The application of LC-MS/MS in pesticide residue analysis has become increasingly popular due to its versatility, specicity, and selectivity, which enables the detection of target compounds in the low ng/L range [17], and also be free of interferences from matrix components present in complex samples such as plant, tissue, and soil.…”

Section: Introductionmentioning

confidence: 99%

Stereoselective separation and determination of triadimefon and triadimenol in wheat, straw, and soil by liquid chromatography–tandem mass spectrometry

Liang

Qiu

et al. 2011

J of Separation Science

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A sensitive and rapid analytical method was developed for simultaneous determination of triadimefon (TF) and triadimenol (TN) stereoisomers in wheat, straw, and soil by liquid chromatography coupled with triple quadrupole mass spectrometry (LC-MS/MS). The direct enantioseparation of TF and TN was performed on a Lux cellulose-1 column packed with cellulose-tris-(3,5-dimethylphenylcarbamate). The effects of mobile-phase composition on the separation were investigated and stereoisomeric elution orders were confirmed with a polarimeter detector. The pesticides were extracted from samples with acetonitrile and cleaned up by solid-phase extraction or activated carbon. Based on the developed stereoselective LC-MS/MS method, for TF and TN stereoisomers, good linearities were obtained over the concentration range of 0.003-4 mg/L; recoveries were 84.2-102.7% in wheat, 84.0-104.0% in straw, and 85.2-106.8% in soil at spiked concentrations of 0.007-2.0 mg/kg; intra-day and inter-day assay precisions were below 12.2%. Limits of detection (LODs) and limits of quantification (LOQs) in wheat, straw, and soil were 0.001-0.005 mg/kg and 0.007-0.02 mg/kg, respectively. Finally, the method was successfully applied to detect TF and TN stereoisomers in wheat, straw, and soil samples from residual trials in farm.

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Reversed-phase chiral HPLC and LC/MS analysis with tris(chloromethylphenylcarbamate) derivatives of cellulose and amylose as chiral stationary phases

Cited by 124 publications

References 40 publications

Test-set reduction in the screening step definition of a chiral separation strategy in polar organic solvents chromatography

Test-set reduction in the screening step definition of a chiral separation strategy in polar organic solvents chromatography

Chiral separation of (d)- and (l)-enantiomers of doxylamine succinate in rat plasma

Stereoselective separation and determination of triadimefon and triadimenol in wheat, straw, and soil by liquid chromatography–tandem mass spectrometry

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