Reversed micelle solvents as tools of enzyme purification and enzyme-catalyzed conversion

Tonova, Konstantza; Lazarova, Z.

doi:10.1016/j.biotechadv.2008.06.002

Cited by 112 publications

(61 citation statements)

References 180 publications

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“…According to Figure 11, the reaction in microemulsion proceeds significantly faster than in solution, which could be explained by a super-activity of the enzyme, often observed in microemulsions [70][71][72]. This conclusion is in agreement with the previous observations of the increase in the hydrolytic activity of the α-amylase against the oligomer substrates in a presence of surfactants [42].…”

Section: Hr-us Monitoring Of Enzyme Catalyzed Reactions In Nanodropletssupporting

confidence: 82%

Ultrasonic Monitoring of Biocatalysis in Solutions and Complex Dispersions

Buckin

Altas

2017

Catalysts

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Abstract:The rapidly growing field of chemical catalysis is dependent on analytical methods for non-destructive real-time monitoring of chemical reactions in complex systems such as emulsions, suspensions and gels, where most analytical techniques are limited in their applicability, especially if the media is opaque, or if the reactants/products do not possess optical activity. High-resolution ultrasonic spectroscopy is one of the novel technologies based on measurements of parameters of ultrasonic waves propagating through analyzed samples, which can be utilized for real-time non-invasive monitoring of chemical reactions. It does not require optical transparency, optical markers and is applicable for monitoring of reactions in continuous media and in micro/nano bioreactors (e.g., nanodroplets of microemulsions). The technology enables measurements of concentrations of substrates and products over the whole course of reaction, analysis of time profiles of the degree of polymerization and molar mass of polymers and oligomers, evolutions of reaction rates, evaluation of kinetic mechanisms, measurements of kinetic and equilibrium constants and reaction Gibbs energy. It also provides tools for assessments of various aspects of performance of catalysts/enzymes including inhibition effects, reversible and irreversible thermal deactivation. In addition, ultrasonic scattering effects in dispersions allow real-time monitoring of structural changes in the medium accompanying chemical reactions.

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Section: Hr-us Monitoring Of Enzyme Catalyzed Reactions In Nanodropletssupporting

confidence: 82%

Ultrasonic Monitoring of Biocatalysis in Solutions and Complex Dispersions

Buckin

Altas

2017

Catalysts

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“…Surprisingly, Roy et al [45] demonstrated that increase of n-hexanol in isooctane/CTAB/n-hexanol/water system enhances the activity of HRP towards the oxidation of pyrogallol at pH = 7, even though alcohols are inhibitors for this enzyme. water phases may disturb the encapsulated enzyme functionality [56]. In the case of microemulsions loaded with oxidative enzymes, increase of alcohol chain length leads to a bell shaped curve versus the catalytic activity, with the lowest reaction rates in dodecane/SDS/n-alcohol/buffer system recorded for 1-octanol and 1-butanol, respectively ( Figure 2).…”

Section: Effect Of Co-surfactantmentioning

confidence: 99%

“…These molecules are called co-surfactants and affect both microemulsion size and structure as their short alkyl chains strongly influence the interfacial composition [52][53][54][55]. In some cases the partition of the co-surfactant between the oil and water phases may disturb the encapsulated enzyme functionality [56]. In the case of microemulsions loaded with oxidative enzymes, increase of alcohol chain length leads to a bell shaped curve versus the catalytic activity, with the lowest reaction rates in dodecane/SDS/n-alcohol/buffer system recorded for 1-octanol and 1-butanol, respectively (Figure 2).…”

Section: Effect Of Co-surfactantmentioning

confidence: 99%

Oxidation Catalysis by Enzymes in Microemulsions

2017

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Microemulsions are regarded as "the ultimate enzyme microreactors" for liquid oxidations. Their structure, composed of water nanodroplets dispersed in a non-polar medium, provides several benefits for their use as media for enzymatic transformations. They have the ability to overcome the solubility limitations of hydrophobic substrates, enhance the enzymatic activity (superactivity phenomenon) and stability, while providing an interface for surface-active enzymes. Of particular interest is the use of such systems to study biotransformations catalyzed by oxidative enzymes. Nanodispersed biocatalytic media are perfect hosts for liquid oxidation reactions catalyzed by many enzymes such as heme peroxidases, phenoloxidases, cholesterol oxidase, and dehydrogenases. The system's composition and structural properties are important for better understanding of nanodispersion-biocatalyst interactions.

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“…Recently, gemini surfactants have been received much attention due their wide applications in industry, biochemistry, pharmacy, and cosmetics. 9 Gemini surfactant belongs to a new class of surfactants, containing two hydrophilic head group and two hydrophobic tails and a spacer in the same molecule. [10][11][12][13] The lower critical micellar concentration (CMC) value of gemini surfactants over the conventional surfactants comprises the better wetting, foaming, dispersing, and emulsifying properties.…”

Section: Introductionmentioning

confidence: 99%

Molecular investigation of the interaction between ionic liquid type gemini surfactant and lysozyme: A spectroscopic and computational approach

et al. 2015

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Herein, we are reporting the interaction of ionic liquid type gemini surfactant, 1,4-bis(3-dodecylimidazolium-1-yl) butane bromide ([C12-4-C12 im]Br2) with lysozyme by using Steady state fluorescence, UV-visible, Time resolved fluorescence, Fourier transform-infrared (FT-IR) spectroscopy techniques in combination with molecular modeling and docking method. The steady state fluorescence spectra suggested that the fluorescence of lysozyme was quenched by [C12-4-C12 im]Br2 through static quenching mechanism as confirmed by time resolved fluorescence spectroscopy. The binding constant for lysozyme-[C12-4-C12 im]Br2 interaction have been measured by UV-visible spectroscopy and found to be 2.541 × 10(5) M(-1). The FT-IR results show conformational changes in the secondary structure of lysozyme by the addition of [C12-4-C12 im]Br2. Moreover, the molecular docking study suggested that hydrogen bonding and hydrophobic interactions play a key role in the protein-surfactant binding. Additionally, the molecular dynamic simulation results revealed that the lysozyme-[C12-4-C12 im]Br2 complex reaches an equilibrium state at around 3 ns.

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Reversed micelle solvents as tools of enzyme purification and enzyme-catalyzed conversion

Cited by 112 publications

References 180 publications

Ultrasonic Monitoring of Biocatalysis in Solutions and Complex Dispersions

Ultrasonic Monitoring of Biocatalysis in Solutions and Complex Dispersions

Oxidation Catalysis by Enzymes in Microemulsions

Molecular investigation of the interaction between ionic liquid type gemini surfactant and lysozyme: A spectroscopic and computational approach

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