Reversed end Ds element: a novel tool for chromosome engineering in Arabidopsis

Krishnaswamy, Lakshminarasimhan; Zhang, Jian Bo; Peterson, Thomas

doi:10.1007/s11103-008-9377-6

Cited by 15 publications

(7 citation statements)

References 53 publications

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“…Previous research has identified examples of "fused ends"; i.e., cases in which the ITS is deleted and the 59 and 39 Ac/Ds termini are ligated together (Gorbunova and Levy 1997;Krishnaswamy et al 2008). The presence of fused ends among the mutant alleles tested here could be detected by PCR with Ac 59 end primer (Ac120r) and Ac 39 end primer (Ac4436f) ( Table 2).…”

Section: A Translocations and Large Inversions (Five Cases)mentioning

confidence: 73%

Genome Rearrangements in Maize Induced by Alternative Transposition of ReversedAc/DsTermini

Yu¹,

Zhang

Peterson

2011

Genetics

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Alternative transposition can induce genome rearrangements, including deletions, inverted duplications, inversions, and translocations. To investigate the types and frequency of the rearrangements elicited by a pair of reversed Ac/Ds termini, we isolated and analyzed 100 new mutant alleles derived from two parental alleles that both contain an intact Ac and a fractured Ac ( fAc) structure at the maize p1 locus. Mutants were characterized by PCR and sequencing; the results show that nearly 90% (89/100) of the mutant alleles represent structural rearrangements including deletions, inversions, translocations, or rearrangement of the intertransposon sequence (ITS). Among 37 deletions obtained, 20 extend into the external flanking sequences, while 17 delete portions of the intertransposon sequence. Interestingly, one deletion allele that contains only a single nucleotide between the retained Ac and fAc termini is not competent for further alternative transposition events. We propose a new model for the formation of intertransposon deletions through insertion of reversed transposon termini into sister-chromatid sequences. These results document the types and frequencies of genome rearrangements induced by alternative transposition of reversed Ac/Ds termini in maize.

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Section: A Translocations and Large Inversions (Five Cases)mentioning

confidence: 73%

Genome Rearrangements in Maize Induced by Alternative Transposition of ReversedAc/DsTermini

Yu¹,

Zhang

Peterson

2011

Genetics

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“…(Dooner and Weil 2007;Huang and Dooner 2008). Another study has shown that chromosomal rearrangements including deletion, inversion, and somatic translocation can also arise through transposition reactions involving Ds elements in Arabidopsis (Krishnaswamy et al 2008). In all of these cases, the rearrangement junctions have been precise or nearly so.…”

Section: Discussionmentioning

confidence: 99%

Alternative Ac/Ds transposition induces major chromosomal rearrangements in maize

Zhang¹,

Yu²,

Pulletikurti³

et al. 2009

Genes Dev.

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Barbara McClintock reported that the Ac/Ds transposable element system can generate major chromosomal rearrangements (MCRs), but the underlying mechanism has not been determined. Here, we identified a series of chromosome rearrangements derived from maize lines containing pairs of closely linked Ac transposable element termini. Molecular and cytogenetic analyses showed that the MCRs in these lines comprised 17 reciprocal translocations and two large inversions. The breakpoints of all 19 MCRs are delineated by Ac termini and characteristic 8-base-pair target site duplications, indicating that the MCRs were generated by precise transposition reactions involving the Ac termini of two closely linked elements. This alternative transposition mechanism may have contributed to chromosome evolution and may also occur during V(D)J recombination resulting in oncogenic translocations.[Keywords: V(D)J recombination; chromosome rearrangements; hAT elements; transposition] Supplemental material is available at http://www.genesdev.org.

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“…In maize, chromosomal aberrations induced by the Ac/Ds system were initially identified by loss of kernel pericarp or aleurone pigmentation specified by the p1 and bz1 genes, respectively (5,7,9). In Arabidopsis and rice, candidate rearrangement events were detected through loss of selectable marker genes included in the Ds- containing transgene construct (39; C. Yu et al . 2011, submitted for publication).…”

Section: Discussionmentioning

confidence: 99%

Transposon Ac/Ds -induced chromosomal rearrangements at the rice OsRLG5 locus

Xuan

Piao

et al. 2011

Nucleic Acids Research

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Previous studies have shown that pairs of closely-linked Ac/Ds transposable elements can induce various chromosomal rearrangements in plant genomes. To study chromosomal rearrangements in rice, we isolated a line (OsRLG5-161) that contains two inversely-oriented Ds insertions in OsRLG5 (Oryza sativa Receptor like kinase Gene 5). Among approximately 300 plants regenerated from OsRLG5-161 heterozygous seeds, 107 contained rearrangements including deletions, duplications and inversions of various sizes. Most rearrangements were induced by previously identified alternative transposition mechanism. Furthermore, we also detected a new class of rearrangements that contain juxtaposed inversions and deletions on the same chromosome. We propose that these novel alleles were generated by a previously unreported type of alternative transposition reactions involving the 5′ and 3′ termini of two inversely-oriented Ds elements located on the same chromatid. Finally, 11% of rearrangements contained inversions resulting from homologous recombination between the two inverted Ds elements in OsRLG5-161. The high frequency inheritance and great variety of rearrangements obtained suggests that the rice regeneration system results in a burst of transposition activity and a relaxation of the controls which normally limit the transposition competence of individual Ds termini. Together, these results demonstrate a greatly enlarged potential of the Ac/Ds system for plant chromosome engineering.

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Reversed end Ds element: a novel tool for chromosome engineering in Arabidopsis

Cited by 15 publications

References 53 publications

Genome Rearrangements in Maize Induced by Alternative Transposition of ReversedAc/DsTermini

Genome Rearrangements in Maize Induced by Alternative Transposition of ReversedAc/DsTermini

Alternative Ac/Ds transposition induces major chromosomal rearrangements in maize

Transposon Ac/Ds -induced chromosomal rearrangements at the rice OsRLG5 locus

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