Reverse transcription-polymerase chain reaction and immunohistochemical study of the expression of fibronectin mRNA in human submandibular salivary gland

Doi, Satoshi; Kawarada, Masaya; Hirano, Hideyasu; Takeuchi, Hiroshi

doi:10.1016/s0003-9969(98)00035-1

Cited by 1 publication

(1 citation statement)

References 27 publications

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“…However, no common design protocols of PCR-Primer and Antisense-Primer exist. We use the fuzzy deducing checking protocol [2][3][4][5][6][7][8][9][10][11][12][13][14]. And we designed various high specific primers, easily and quickly.…”

Section: Introductionmentioning

confidence: 99%

Design of RT-PCR Primers Based on Informational Method

Makino¹,

Hirano²

First International Conference on Innovative Computing, Information and Control - Volume I (ICICIC'06)

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To detect mRNA expression levels were very useful not only for the biological studies but also for the progress of medical therapy. Furthermore, mRNA transcription suppression caused by antisense also clearized and made possible the speedy result of the cell biological investigation. We created primer design protocol using fuzzy deducing theory, because the most of the traditional computer protocol succeeded less than 20%. The 90% of our primers functioned after following checking (1. GC Contents Check; 2. Homologous Check: [versus a.: GAPDH, b: Profilin,; 3. Self-Homology Check;). We also added 4: 5'-, & -3' homology checking, and 5: gene family checking to target into a real mRNA out of similar sequences. Fuzzy deducing checking protocol made it possible to select real VEGF mRNA from gene family in which there are many similar sequences.

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Section: Introductionmentioning

confidence: 99%

Design of RT-PCR Primers Based on Informational Method

Makino¹,

Hirano²

First International Conference on Innovative Computing, Information and Control - Volume I (ICICIC'06)

View full text Add to dashboard Cite

show abstract

Reverse transcription-polymerase chain reaction and immunohistochemical study of the expression of fibronectin mRNA in human submandibular salivary gland

Cited by 1 publication

References 27 publications

Design of RT-PCR Primers Based on Informational Method

Design of RT-PCR Primers Based on Informational Method

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