Reverse transcription and polymerase chain reaction: principles and applications in dentistry

Santos, Carlos; Sakai, Vivien Thiemy; Machado, Maria Aparecida de Andrade Moreira; Schippers, Daniela Nicole; Greene, Andrew S.

doi:10.1590/s1678-77572004000100002

Cited by 26 publications

(35 citation statements)

References 61 publications

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“…The most popular methods are RT-PCR/multiplex RT-PCR and qRT-PCR (quantitative real-time PCR). They rely on specific primers/probes and require known sequences in the target nucleic acid (Santos et al, 2004;Arya et al, 2005). Microarrays and multiplex PCR come in handy when one needs to analyze different viruses in one experiment (Wang et al, 2002;Chen et al, 2004;Liu et al, 2005;Topley et al, 2005;Glover et al, 2011;Sguazza et al, 2013).…”

Section: Viral Infections -Detection Nucleic Acid-based Assaysmentioning

confidence: 99%

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Viral infections of Apis mellifera : its importance, dynamics, diagnostics and treatment opportunities

Matusiewicz¹,

Margas²,

Mazurek³

et al. 2016

bta

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Honey bees (Apis mellifera ) play a crucial function in terrestrial ecosystems by maintaining floral biodiversity and providing sustainable agriculture through pollination. Bees affect 35% of the world's crop production, hence, they are essential for economic sustainability, agriculture and food security. The economic value of pollination by bees goes beyond agricultural production. An important role of bees is pollination of the plants that provide food to the wild animals, and this is part of the proper functioning of the ecosystem. On the one hand Apis mellifera has great adaptive potential and it may be found in highly diverse climates almost everywhere in the world, but on the other hand bees are under constant threat by numerous pathogens including bacteria, viruses, fungi, Microsporidia, mites and insects. Other stress factors include environmental change-related stresses, pesticides, malnutrition and migratory beekeeping. The interactions among these, along with possibly other unknown factors, are causing widespread decline in honey bee populations -Colony Collapse Disorder (CCD) syndrome. Viruses are the most recent and still poorly investigated pathogens of the honey bees. Simultaneously, Varroa destructor mites and viruses are currently the high-profile suspects in collapsing bee colonies. Varroa acts as a vector for a range of RNA viruses of honey bees which are transferred when the mite feeds on hemolymph from the developing pupa. This publication aims to present an overview of the most important honey bee viruses, their diagnostics methods and treatment opportunities with emphasis on the most common and devastating viruses from Dicistroviridae and Iflaviridae families.

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Section: Viral Infections -Detection Nucleic Acid-based Assaysmentioning

confidence: 99%

“…The first-response diagnostics done by beekeepers in place is not possible with the use of such methods (Santos et al, 2004;Arya et al, 2005).…”

Section: Viral Infections -Detection Nucleic Acid-based Assaysmentioning

confidence: 99%

Viral infections of Apis mellifera : its importance, dynamics, diagnostics and treatment opportunities

Matusiewicz¹,

Margas²,

Mazurek³

et al. 2016

bta

View full text Add to dashboard Cite

show abstract

“…The teeth are an excellent source of genomic DNA because PCR analysis allow comparing the collected postmortem samples to known antemortem samples or parental DNA [18,19]. Mitochondrial DNA is another type of material that can be used for body identification.…”

Section: Genomic and Mitochondrial Dna In Forensic Dentistrymentioning

confidence: 99%

Use of DNA Technology in Forensic Dentistry

Shambulingappa¹,

Soheyl²,

Gupta³

et al. 2012

J Forensic Res

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The discovery of DNA plays vital role in Human identification is one of the major fields of study and research in forensic science. This discovery was the basis for the development of techniques that allow characterizing each person's individuality based on the DNA sequence. Individual identification of ancient human remains the most fundamental requisites for studies of paleo-population genetics, including kinship among ancient people, intra-and inter population structures in ancient times, and the origin of human populations. However, knowledge of these subjects has been based mainly on circumstantial archaeological evidence for kinship and intra population structure and on genetic studies of modern human populations. Tooth and several biological materials such as bone tissue, hair bulb, saliva, blood and other body tissues may be employed for isolation of DNA and accomplishment of laboratory tests for human identification. Here we describe individual identification of ancient humans by using short-nucleotide tandem repeats and mitochondrial DNA (mtDNA) extracted from a tooth as genetic markers. The application of this approach to kinship analysis shows clearly the presence or absence of kinship among the ancient remains examined.

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“…It has recently become a standard diagnostic and research tool in dentistry. The literature reports the uses of PCR for the study of factors involved in periodontal disease, dental caries, endodontic infections and oral cancer (90). …”

Section: Principles and Applications In Dentistrymentioning

confidence: 99%

Principles and applications of polymerase chain reaction in medical diagnostic fields: a review

Valones¹,

Guimarães²,

Brandão³

et al. 2009

Braz. J. Microbiol.

146

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Recent developments in molecular methods have revolutionized the detection and characterization of microorganisms in a broad range of medical diagnostic fields, including virology, mycology, parasitology, microbiology and dentistry. Among these methods, Polymerase Chain Reaction (PCR) has generated great benefits and allowed scientific advancements. PCR is an excellent technique for the rapid detection of pathogens, including those difficult to culture. Along with conventional PCR techniques, Real-Time PCR has emerged as a technological innovation and is playing an ever-increasing role in clinical diagnostics and research laboratories. Due to its capacity to generate both qualitative and quantitative results, Real-Time PCR is considered a fast and accurate platform. The aim of the present literature review is to explore the clinical usefulness and potential of both conventional PCR and Real-Time PCR assays in diverse medical fields, addressing its main uses and advances.

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Reverse transcription and polymerase chain reaction: principles and applications in dentistry

Cited by 26 publications

References 61 publications

Viral infections of Apis mellifera : its importance, dynamics, diagnostics and treatment opportunities

Viral infections of Apis mellifera : its importance, dynamics, diagnostics and treatment opportunities

Use of DNA Technology in Forensic Dentistry

Principles and applications of polymerase chain reaction in medical diagnostic fields: a review

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