1997
DOI: 10.1089/aid.1997.13.601
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Reverse Transcriptase and Corresponding Activity-Blocking Antibody for Monitoring SIVInfection in Macaques

Abstract: A nonradioactive reverse transcriptase (RT) assay was used to measure RT activity in serum during the viremia peak associated with primary infection and for measuring the generation and maintenance of RT activity-blocking antibody (RTb-ab) titers during and after seroconversion in SIV-infected macaques. The RT assay was compared to an antigen capture immunoassay designed for HIV-2/SIVsm and was found to be approximately 40 times more sensitive in detecting SIVsm in serum from infected macaques. The RT assay de… Show more

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Cited by 10 publications
(9 citation statements)
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“…The RT uses the viral RNA genome as template to produce viral DNA prior to integration in to the host cell genome. This process can be measured in vitro using RNA template and a dNTP analogue (such as BrdUTP) together with colorimetric product [122][123][124], see Figure 7.…”
Section: Rt Assays For Vl Quantification and Resistance Testingmentioning
confidence: 99%
“…The RT uses the viral RNA genome as template to produce viral DNA prior to integration in to the host cell genome. This process can be measured in vitro using RNA template and a dNTP analogue (such as BrdUTP) together with colorimetric product [122][123][124], see Figure 7.…”
Section: Rt Assays For Vl Quantification and Resistance Testingmentioning
confidence: 99%
“…Briefly, serial 10-fold dilutions of the plasma were added to 0.5 ϫ 10 6 SupT1 cells (four wells per dilution). Virus production was measured by detection of reverse transcription (RT) activity (Cavidi Tech, Uppsala, Sweden) in the supernatant of each well at days 14 and 28 of culture (19). The infectious titer of the plasma was then expressed as the 50% tissue culture infectious dose (TCID 50 ) determined by the Karber calculation method.…”
Section: Animalsmentioning
confidence: 99%
“…Preliminary results with the current assays indicate at least a 10-fold difference in the sensitivity of the RT for the same isolates (unpublished results). Furthermore, as RT activity can be detected directly in plasma during primary simian immunodeficiency virus viraemia in macaques and primary HIV infection in man (Awad et al, 1997;Ekstrand et al, 1997), and even in later stages (unpublished results) with the current assay, it is of interest to try to improve the sensitivity of the assay in order to achieve a technique useful for direct analyses of therapy resistance based on the RT present in the bloodstream.…”
Section: Antiviral Chemistry Andchemotherapy 8(2) Discussionmentioning
confidence: 99%