Reverse relation between cytotoxicity and Polyethylenimine/DNA ratio, the effect of using HEPES-buffered saline (HBS) medium in gene delivery

Dabbaghi, Maryam; Hashemi, Khadijeh; Oskuee, Reza Kazemi; Afkhami-Goli, Amir

doi:10.1016/j.tiv.2022.105414

Cited by 3 publications

(3 citation statements)

References 30 publications

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“…This kit assesses living cells that produce NAD(P)H to convert WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H tetrazolium, monosodium salt) into formazan, which can be detected by its blue light absorbance 19 . We used standard conditions for inducing expression of FPs by PEI-mediated transient transfection of HeLa cells in a 96-well format 11,20 . On day 0, 3,000 HeLa cells were seeded per well in 100 µl DMEM in a Greiner (cat.…”

Section: Photochromicitymentioning

confidence: 99%

mScarlet3: a brilliant and fast-maturing red fluorescent protein

et al. 2023

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Publisher's note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

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Section: Photochromicitymentioning

confidence: 99%

mScarlet3: a brilliant and fast-maturing red fluorescent protein

et al. 2023

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“…[ 112 ] Nevertheless, cationic polymers are difficult to degrade in vivo, which reduces the biosafety of the vector. [ 113 ] More importantly, cationic polymers bind to nucleic acids based on electrostatic interactions, and gene release is not triggered by the specific tumor microenvironment, which would lead to a sharp decline in gene therapy efficacy. [ 114 ] Co‐assembly of polyphenols with cationic polymers is helpful to maximize transfection efficiency while limiting the cytotoxicity of cationic polymers.…”

Section: Polyphenol‐enhanced Multimodal Cancer Therapymentioning

confidence: 99%

Polyphenol‐Based Nanosystems for Next‐Generation Cancer Therapy: Multifunctionality, Design, and Challenges

Feng,

Chen,

Fan

et al. 2023

Macromolecular Bioscience

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With the continuous updating of cancer treatment methods and the rapid development of precision medicine in recent years, there are higher demands for advanced and versatile drug delivery systems. Scientists are committed to create greener and more effective nanomedicines where the carrier is no longer limited to a single function of drug delivery. Polyphenols, which can act as both active ingredients and fundamental building blocks, are being explored as potential multifunctional carriers that are efficient and safe for design purposes. Due to their intrinsic anticancer activity, phenolic compounds have shown surprising expressiveness in ablation of tumor cells, overcoming cancer multidrug resistance (MDR), and enhancing immunotherapeutic efficacy. This review provides an overview of recent advances in the design, synthesis, and application of versatile polyphenol‐based nanosystems for cancer therapy in various modes. Moreover, the merits of polyphenols and the challenges for their clinical translation are also discussed, and it is pointed out that the novel polyphenol delivery system requires further optimization and validation.

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“…Typical examples of non-viral vectors include complexes of plasmid DNA with cationic polymers or liposomes. In particular, various types of polymers are used for gene delivery, including polyethyleneimine [ 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 ], poly-β-amino esters [ 15 , 16 , 17 , 18 ], chitosan [ 19 , 20 , 21 , 22 ], and dendrimers [ 23 , 24 ]. These complexes have been reported to exhibit high gene expression in vitro and in vivo.…”

Section: Introductionmentioning

confidence: 99%

Interaction of γ-Polyglutamic Acid/Polyethyleneimine/Plasmid DNA Ternary Complexes with Serum Components Plays a Crucial Role in Transfection in Mice

Ko,

Fumoto,

Kurosaki

et al. 2024

Pharmaceutics

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Typical examples of non-viral vectors are binary complexes of plasmid DNA with cationic polymers such as polyethyleneimine (PEI). However, problems such as cytotoxicity and hemagglutination, owing to their positively charged surfaces, hinder their in vivo use. Coating binary complexes with anionic polymers, such as γ-polyglutamic acid (γ-PGA), can prevent cytotoxicity and hemagglutination. However, the role of interactions between these complexes and serum components in in vivo gene transfer remains unclear. In this study, we analyzed the contribution of serum components to in vivo gene transfer using PEI/plasmid DNA binary complexes and γ-PGA/PEI/plasmid DNA ternary complexes. In binary complexes, heat-labile components in the serum greatly contribute to the hepatic and splenic gene expression of the luciferase gene. In contrast, serum albumin and salts affected the hepatic and splenic gene expression in the ternary complexes. Changes in physicochemical characteristics, such as increased particle size and decreased absolute values of ζ-potential, might be involved in the enhanced gene expression. These findings would contribute to a better understanding of in vivo non-viral gene transfer using polymers, such as PEI and γ-PGA.

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Reverse relation between cytotoxicity and Polyethylenimine/DNA ratio, the effect of using HEPES-buffered saline (HBS) medium in gene delivery

Cited by 3 publications

References 30 publications

mScarlet3: a brilliant and fast-maturing red fluorescent protein

mScarlet3: a brilliant and fast-maturing red fluorescent protein

Polyphenol‐Based Nanosystems for Next‐Generation Cancer Therapy: Multifunctionality, Design, and Challenges

Interaction of γ-Polyglutamic Acid/Polyethyleneimine/Plasmid DNA Ternary Complexes with Serum Components Plays a Crucial Role in Transfection in Mice

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