Reverse Phase Liquid Chromatography for Cetrimide Determination in Pure and Pharmaceutical Preparations
Ziad T I Alkayar,
Ahmed A.Ismail,
Nagham Muthanna Jassim Al-Ani
et al.
Abstract:An effective, duplicable, and quick method to determine the purity and formulating form of Cetrimide (CE) have studied and modified utilizing the Reverse Phase High Performance Liquid Chromatography (RP-HPLC). The method was based on the separation of the CE using a reverse phase HPLC Shimadzu LC–20 A, Japan, and Phenomenex C18 column (15 × 4.6mm, 5.0μm). The process was carried out by using the mobile phase ammonium acetate solution 0.1M (AA), methanol (MeOH), and the methyl cyanide in the ratio of (15:60:25 … Show more
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