Reverse-phase high performance liquid chromatography for the determination of tiopronin in human plasma after derivatization with p-bromophenacyl bromide

Ultraviolet derivatization of low-molecular-mass thiols for high performance liquid chromatography and capillary electrophoresis analysis

“…Captopril derivative was detected at 260 nm. Optimal reaction pH of MPG with pBPB occurred at 14 and was complete at 20 • C after 30 min [100]. A sufficiently high concentration of pBPB was required for the reaction to proceed efficiently.…”

“…It should be noted that mBrB and its hydrolysis products give multiple peaks on the chromatograms [96]. For derivatization of thiols, represented by captopril and 2-mercaptopropionylglycine, p-bromophenacyl bromide (pBPB) was used [98][99][100][101]. pBPB reacts with captopril to form a product that shows ultraviolet-absorbing properties [98,99].…”

Stereoselective analysis of tiopronin enantiomers in rat plasma using high‐performance liquid chromatography‐electrospray ionization mass spectrometry after chiral derivatization

“…For this reason, it is essential to establish a simple and practical quantitation method to separate and measure the isomers of tiopronin and then apply to further pharmacokinetic study. A variety of achiral methods has been established for the analysis of tiopronin in the biological matrices, including high-performance liquid chromatography (HPLC), 5,6 chemiluminescence, 7 and liquid chromatography-mass spectrometry (LC-MS). 8 Compared with the extensive literatures on the analysis of racemic tiopronin, no report is available on the analysis the enantiomers of tiopronin in biological matrices.…”

Cadmium telluride quantum dots as pH-sensitive probes for tiopronin determination

“…A number of spectrometric [20], chromatographic [21][22][23][24][25] and chemiluminescent [26,27] methods had been proposed for its determination. However, the above methods are expensive, time-consuming or requiring complex mathematic dealing.…”