Reverse mutants of the nitrate reductase-deficient mutant B 25 of Arabidopsis thaliana

Braaksma, F. J.; Feenstra, W.J.

doi:10.1007/bf00273784

Cited by 6 publications

(1 citation statement)

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“…(1) Back mutation and (2) suppressor mutation. Braaksma and Feenstra (1982b) found revertants of mutant B25 with high frequency. Reversion was caused by mu-tations in a suppressor gene, which were either recessive or dominant.…”

Section: Discussionmentioning

confidence: 97%

Nitrate reductase deficient cell lines from diploid cell cultures and lethal mutant M2 plants of Arabidopsis thaliana

Scholten

Vries

Nijdam

et al. 1985

Theoret. Appl. Genetics

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Cell suspensions of diploid Arabidopsis thaliana were screened for resistance to chlorate on a medium with ammonium nitrate as the nitrogen source, and after plating on filters to increase the plating efficiency. Thirty-nine lines were selected, four of which were still resistant after two years of subculturing on non-selective medium. Of the latter lines three were nitrate reductase deficient but exhibited some residual nitrate reductase activity; the fourth line showed a high level of enzyme activity. Screening M2-seeds for callus production on selective medium with amino acids as the nitrogen source and chlorate revealed resistant calli in 17 out of 483 M2-groups. Nine well-growing lines, all but one (G3) exhibiting no detectable in vivo nitrate reductase activity, were classified as defective in the cofactor. Two lines (G1 and G3) could be analysed genetically at the plant level. Chlorate resistance was monogenic and recessive. Sucrose gradient fractionation of callus extracts of G1 revealed that a complete enzyme molecule can be assembled. Nitrate reductase activity in G1 could partly be restored by excess molybdenum. It is suggested that G1 is disturbed in the catalytic properties of the cofactor. It appeared that G1 is neither allelic with another molybdenum repairable mutant (B73) nor with another cofactor mutant (B25). Wilting of intact G1 plants could be ascribed to non-closing stomata.

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Section: Discussionmentioning

confidence: 97%