Reverse line blot hybridisation screening of <i>Pseudallescheria/Scedosporium</i> species in patients with cystic fibrosis

Lu, Qiaoyun; Ende, A. H. Gerrits Van Den; Hoog, G. Sybren de; Li, R.Y.; Accoceberry, Isabelle; Durand-Joly, Isabelle; Bouchara, Jean Philippe; Hernandez, F.; Delhaès, Laurence

doi:10.1111/j.1439-0507.2011.02108.x

Cited by 19 publications

(9 citation statements)

References 24 publications

(59 reference statements)

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“…However, they have some limitations in identifying co-infections and dynamics of polymicrobial populations. These communities consist of bacteria, fungi, and viruses all potentially contributing to infection and inflammation ( Lu et al, 2011 ; Charlson et al, 2012a , b ; Delhaes et al, 2012 ; Fodor et al, 2012 ; Filkins et al, 2012 ; Carmody et al, 2013 ; Cui et al, 2013 ; Harrison et al, 2013 ; Huang et al, 2013 ; Dawood et al, 2014 ; Fitzpatrick et al, 2014 ; Goffard et al, 2014 ; Lim et al, 2014 ; McCullers, 2014 ; Mounier et al, 2014 ; Purcell et al, 2014 ; Willger et al, 2014 ; Wurzel et al, 2014 ). In addition, the majority of fungal species are difficult to cultivate or uncultivable on usual culture media as well as new or unknown pathogens ( Bittar et al, 2008 ; Chabé et al, 2011 ; Paniz-Mondolfi et al, 2012 ).…”

Section: Respiratory Mycobiome: From Proof Of Concept To Rational Datmentioning

confidence: 99%

“…Despite the fact that knowledge of the mycobiome lags behind our understanding of the bacterial microbiome, specific mycobiota have been identified in pulmonary diseases as well as in oral, digestive, and skin diseases ( Ghannoum et al, 2010 ; Lu et al, 2011 ; Charlson et al, 2012b ; Delhaes et al, 2012 ; Knutsen et al, 2012 ; Harrison et al, 2013 ; Huang et al, 2013 ; Dupuy et al, 2014 ; Willger et al, 2014 ). Currently, the abundance of fungi in different body sites seems to be many degrees smaller than bacteriome as recently reported ( Marsland and Gollwitzer, 2014 ; Underhill and Iliev, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

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The lung mycobiome: an emerging field of the human respiratory microbiome

2015

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The lung microbiome, which is believed to be stable or at least transient in healthy people, is now considered as a poly-microorganism component contributing to disease pathogenesis. Most research studies on the respiratory microbiome have focused on bacteria and their impact on lung health, but there is evidence that other non-bacterial organisms, comprising the viruses (virome) and fungi (mycobiome), are also likely to play an important role in healthy people as well as in patients. In the last few years, the lung mycobiome (previously named the fungal microbiota or microbiome) has drawn closer attention. There is growing evidence that the lung mycobiome has a significant impact on clinical outcome of chronic respiratory diseases (CRD) such as asthma, chronic obstructive pulmonary disease, cystic fibrosis, and bronchiectasis. Thanks to advances in culture independent methods, especially next generation sequencing, a number of fungi not detected by culture methods have been molecularly identified in human lungs. It has been shown that the structure and diversity of the lung mycobiome vary in different populations (healthy and different diseased individuals) which could play a role in CRD. Moreover, the link between lung mycobiome and different biomes of other body sites, especially the gut, has also been unraveled. By interacting with the bacteriome and/or virome, the respiratory mycobiome appears to be a cofactor in inflammation and in the host immune response, and therefore may contribute to the decline of the lung function and the disease progression. In this review, we report the recent limited explorations of the human respiratory mycobiome, and discuss the mycobiome’s connections with other local microbial communities, as well as the relationships with the different biomes of other body sites. These studies suggest several outlooks for this understudied emerging field, which will certainly call for a renewal of our understanding of pulmonary diseases.

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Section: Respiratory Mycobiome: From Proof Of Concept To Rational Datmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The lung mycobiome: an emerging field of the human respiratory microbiome

2015

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“…As for mycological examination, it requires skill and expertise and may lead to false-negative results for polymicrobial specimens like sputum samples because of the more rapid and more extensive growth of other molds frequently associated with this fungus, like Aspergillus fumigatus (19). Several molecular methods have been proposed for detection of the fungus from sputum samples (20)(21)(22)(23)(24), but as culture methods, they do not allow the differentiation between airway colonization and sensitization of the patient or respiratory infection in the CF context, which has important implications for patient management.…”

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confidence: 99%

Purification and Characterization of a Mycelial Catalase from Scedosporium boydii, a Useful Tool for Specific Antibody Detection in Patients with Cystic Fibrosis

Mina¹,

Marot-Leblond²,

Cimon³

et al. 2014

Clin. Vaccine Immunol.

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bScedosporium boydii is an opportunistic filamentous fungus which may be responsible for a wide variety of infections in immunocompetent and immunocompromised individuals. This fungus belongs to the Scedosporium apiospermum species complex, which usually ranks second among the filamentous fungi colonizing the airways of patients with cystic fibrosis (CF) and may lead to allergic bronchopulmonary mycoses, sensitization, or respiratory infections. Upon microbial infection, host phagocytic cells release reactive oxygen species (ROS), such as hydrogen peroxide, as part of the antimicrobial response. Catalases are known to protect pathogens against ROS by detoxification of the hydrogen peroxide. Here, we investigated the catalase equipment of Scedosporium boydii, one of the major pathogenic species in the S. apiospermum species complex. Three catalases were identified, and the mycelial catalase A1 was purified to homogeneity by a three-step chromatographic process. This enzyme is a monofunctional tetrameric protein of 460 kDa, consisting of four 82-kDa glycosylated subunits. The potential usefulness of this enzyme in serodiagnosis of S. apiospermum infections was then investigated by an enzyme-linked immunosorbent assay (ELISA), using 64 serum samples from CF patients. Whatever the species involved in the S. apiospermum complex, sera from infected patients were clearly differentiated from sera from patients with an Aspergillus fumigatus infection or those from CF patients without clinical and biological signs of a fungal infection and without any fungus recovered from sputum samples. These results suggest that catalase A1 is a good candidate for the development of an immunoassay for serodiagnosis of infections caused by the S. apiospermum complex in patients with CF. Scedosporium boydii (formerly known as Pseudallescheria boydii) is one of the major pathogenic species within the Scedosporium apiospermum complex, which comprises four other species, namely, Scedosporium apiospermum sensu stricto, Scedosporium aurantiacum, Scedosporium minutisporum, and Scedosporium dehoogii, Scedosporium prolificans having been reassigned recently to the genus Lomentospora (Lomentospora prolificans) (1-4). These filamentous fungi are soilborne fungi that may cause a wide range of infections in humans, including subcutaneous mycetomas and ocular, bone, or joint infections resulting from traumatic inoculation of some fungal elements and infections of the respiratory tract (i.e., sinusitis and lung fungus ball), which are thought to be due to the inhalation of some airborne conidia (5-7). However, these fungi have gained attention during the past 2 decades mainly because of their recognition as common agents of colonization of the airways in patients with cystic fibrosis (CF). When appropriate culture media are used, the S. apiospermum species complex ranks second among the filamentous fungi recovered from respiratory specimens, with a prevalence ranging from 4.5 to 11.6% in patients (8-12). Although usually asymptomatic, this fungal colo...

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“…The environmental reservoir of these fungi is uncertain and the epidemiology and mode of transmission are not well defined 16 . This knowledge is significant to CF patients, for example, where Scedosporium is found among the most frequent fungal colonisers of the upper respiratory 17 …”

Section: Introductionmentioning

confidence: 99%

“…16 This knowledge is significant to CF patients, for example, where Scedosporium is found among the most frequent fungal colonisers of the upper respiratory. 17 The aim of the present study was twofold: (1) the selection of simple physiological markers for species recognition in the routine laboratory and (2) the evaluation of a new biotyping system for individual strains. To be able to select from a wide range of distinguishing parameters, three different microtitre platforms for mycological examination (plates A, C and E) of the Taxa Profile system were used, comprising 570 physiological test reactions.…”

Section: Introductionmentioning

confidence: 99%

Physiological typing of Pseudallescheria and Scedosporium strains using Taxa Profile, a semi‐automated, 384‐well microtitre system

Horré

Schaal

Marklein

et al. 2011

Mycoses

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SummaryDuring the last few decades, Pseudallescheria and Scedosporium infections in humans are noted with increasing frequency. Multi-drug resistance commonly occurring in this species complex interferes with adequate therapy. Rapid and correct identification of clinical isolates is of paramount significance for optimal treatment in the early stages of infection, while strain typing is necessary for epidemiological purposes. In view of the development of physiological diagnostic parameters, 570 physiological reactions were evaluated using the Taxa Profile MICRONAUT system, a semi-automatic, computerassisted, 384-well microtitre platform. Thirty two strains of the Pseudallescheria and Scedosporium complex were analysed after molecular verification of correct species attribution. Of the compounds tested, 254 proved to be polymorphic. Cluster analysis was performed with the MICRONAUT profile software, which is linked to the NTSYpcÒ program. The systemic opportunist S. prolificans was unambiguously separated from the remaining species. Within the P. boydii ⁄ P. apiosperma complex differentiation was noted at the level of individual strains, but no unambiguous parameters for species recognition were revealed.

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Reverse line blot hybridisation screening of Pseudallescheria/Scedosporium species in patients with cystic fibrosis

Cited by 19 publications

References 24 publications

The lung mycobiome: an emerging field of the human respiratory microbiome

The lung mycobiome: an emerging field of the human respiratory microbiome

Purification and Characterization of a Mycelial Catalase from Scedosporium boydii, a Useful Tool for Specific Antibody Detection in Patients with Cystic Fibrosis

Physiological typing of Pseudallescheria and Scedosporium strains using Taxa Profile, a semi‐automated, 384‐well microtitre system

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