Reverse homeosis in homeotically reconstructed ribbonworms

Gehring, Walter J.; Bièrne, Jacques

doi:10.1073/pnas.96.21.11900

Cited by 6 publications

(1 citation statement)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1999) or a very small piece from the midbody region. The occurring ‘Janus heads’ (when a second head is formed at the posterior cut surface) or ‘Janus tails’ are caused by inheritable developmental abnormalities (polar heteromorphosis; Tarpin et al . 1999).…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical analysis of nervous system regeneration in chimeric individuals of Dorvillea bermudensis (Polychaeta, Dorvilleidae)

Müller

2004

Dev Growth Differ

View full text Add to dashboard Cite

In regeneration experiments, 0.5% of the two- or five-segmented fragments of the polychaete Dorvillea bermudensis were found unexpectedly transplanted: two fragments of each that were lying close together during the initial period, fused and regenerated a chimeric individual. Of the three theoretical possibilities (i.e. fusion of (i). two posterior ends; (ii). one anterior and one posterior end; (iii). or two anterior ends) only the last two were realized. The similarly oriented fragments regenerated a normal animal while anterior-anterior fused ones produced two heads or a double head. Whether the ventral cords of the fragments are located vis-à-vis or adjacent, influences the course of regeneration as well. Immunohistochemical methods (anti-acetylated alpha-tubulin) in conjunction with confocal laser scanning microscopy were used to investigate the wiring pattern of the nervous systems of the grafts. In all cases, at least two supraesophageal ganglia were formed and palps, antennae and nuchal organs were innervated by the correct nerves but, in special cases, were innervated vice versa from the other brain. From these results it can be concluded that fusion of a regenerating connective with another connective results in formation of a new brain, irrespective of whether it belongs to the same nerve cord or not.

show abstract

Section: Introductionmentioning

confidence: 99%

Immunohistochemical analysis of nervous system regeneration in chimeric individuals of Dorvillea bermudensis (Polychaeta, Dorvilleidae)

Müller

2004

Dev Growth Differ

View full text Add to dashboard Cite

show abstract

Regeneration in planarians and other worms: New findings, new tools, and new perspectives

2002

View full text Add to dashboard Cite

Molecular biology, recombinant DNA techniques, and new methods of cell lineage have reignited the interest of planarians and other worms (mainly annelids and nemerteans) as invertebrate model systems of regeneration. Here, the mean results produced in the last five years are reviewed, an update of the genes and molecules involved in planarian regeneration is provided, and a new morphallactic-epimorphic model of pattern formation is suggested. Moreover, and most importantly, we highlight the new strides brought upon by genomic/proteomic analyses, RNA interference (RNAi) to inactivate gene function, and Bromodeoxyuridine (BrdU) cell labelling. The raising hope to obtain transformed neoblasts and transgenic planarians is also stressed. Altogether, such approaches will eventually lead to solve the long-standing open questions on regeneration which still baffles us. Finally, we warn against overlooking the evident links between regeneration processes and those controlling the daily wear and tear of tissues and cells. Both processes act, at least in planarians, upon a unique stem-cell endowed with an unrivaled developmental potential in the animal kingdom-the neoblast. This cell could be considered the forebear and a model system for stem-cell analysis.

show abstract