Reverse Genetics Systems for Emerging and Re-Emerging Swine Coronaviruses and Applications

Jiang, Hui; Wang, Ting; Kong, Lingbao; Li, Bin; Peng, Qi

doi:10.3390/v15102003

Cited by 3 publications

(2 citation statements)

References 87 publications

(123 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…And these RGSs have been used to study the functions of several PEDV structural and nonstructural proteins and in generating PEDV LAV candidates [50,80,104,114,140,155]. Recently, Jiang et al reviewed the principles, advantages, and drawbacks of the established RGSs for the emerging and re-emerging swine coronaviruses, including PEDV [156]. Thus, we will only provide a brief overview of PEDVs' RGSs.…”

Section: Rgss For Pedvmentioning

confidence: 99%

Developing Next-Generation Live Attenuated Vaccines for Porcine Epidemic Diarrhea Using Reverse Genetic Techniques

Yu,

Dong,

Chen

et al. 2024

Vaccines

View full text Add to dashboard Cite

Porcine epidemic diarrhea virus (PEDV) is the etiology of porcine epidemic diarrhea (PED), a highly contagious digestive disease in pigs and especially in neonatal piglets, in which a mortality rate of up to 100% will be induced. Immunizing pregnant sows remains the most promising and effective strategy for protecting their neonatal offspring from PEDV. Although half a century has passed since its first report in Europe and several prophylactic vaccines (inactivated or live attenuated) have been developed, PED still poses a significant economic concern to the swine industry worldwide. Hence, there is an urgent need for novel vaccines in clinical practice, especially live attenuated vaccines (LAVs) that can induce a strong protective lactogenic immune response in pregnant sows. Reverse genetic techniques provide a robust tool for virological research from the function of viral proteins to the generation of rationally designed vaccines. In this review, after systematically summarizing the research progress on virulence-related viral proteins, we reviewed reverse genetics techniques for PEDV and their application in the development of PED LAVs. Then, we probed into the potential methods for generating safe, effective, and genetically stable PED LAV candidates, aiming to provide new ideas for the rational design of PED LAVs.

show abstract

Section: Rgss For Pedvmentioning

confidence: 99%

Developing Next-Generation Live Attenuated Vaccines for Porcine Epidemic Diarrhea Using Reverse Genetic Techniques

Yu,

Dong,

Chen

et al. 2024

Vaccines

View full text Add to dashboard Cite

show abstract

“…Except for retroviruses, replicons derived from positive-sense RNA viruses do not integrate exogenous genetic information into the host cell genome. Many systems have been described for heterologous gene expression based on infectious cDNA clones of filoviruses [ 7 ], influenza viruses [ 8 ] (reviewed in [ 9 ]), reoviruses [ 10 , 11 , 12 ], rotaviruses [ 13 , 14 ] (reviewed in [ 15 ]), bornaviruses [ 16 ], bunyaviruses [ 17 ], picornaviruses [ 18 ], flaviviruses [ 19 ], alphaviruses [ 20 ], and coronaviruses [ 21 , 22 , 23 , 24 ], among others.…”

Section: Introductionmentioning

confidence: 99%

Highs and Lows in Calicivirus Reverse Genetics

Álvarez,

Arboleya,

Abade dos Santos

et al. 2024

Viruses

View full text Add to dashboard Cite

In virology, the term reverse genetics refers to a set of methodologies in which changes are introduced into the viral genome and their effects on the generation of infectious viral progeny and their phenotypic features are assessed. Reverse genetics emerged thanks to advances in recombinant DNA technology, which made the isolation, cloning, and modification of genes through mutagenesis possible. Most virus reverse genetics studies depend on our capacity to rescue an infectious wild-type virus progeny from cell cultures transfected with an “infectious clone”. This infectious clone generally consists of a circular DNA plasmid containing a functional copy of the full-length viral genome, under the control of an appropriate polymerase promoter. For most DNA viruses, reverse genetics systems are very straightforward since DNA virus genomes are relatively easy to handle and modify and are also (with few notable exceptions) infectious per se. This is not true for RNA viruses, whose genomes need to be reverse-transcribed into cDNA before any modification can be performed. Establishing reverse genetics systems for members of the Caliciviridae has proven exceptionally challenging due to the low number of members of this family that propagate in cell culture. Despite the early successful rescue of calicivirus from a genome-length cDNA more than two decades ago, reverse genetics methods are not routine procedures that can be easily extrapolated to other members of the family. Reports of calicivirus reverse genetics systems have been few and far between. In this review, we discuss the main pitfalls, failures, and delays behind the generation of several successful calicivirus infectious clones.

show abstract

Progress of research on coronaviruses and toroviruses in large domestic animals using reverse genetics systems

Ujike,

Suzuki

2024

The Veterinary Journal

View full text Add to dashboard Cite

Reverse Genetics Systems for Emerging and Re-Emerging Swine Coronaviruses and Applications

Cited by 3 publications

References 87 publications

Developing Next-Generation Live Attenuated Vaccines for Porcine Epidemic Diarrhea Using Reverse Genetic Techniques

Developing Next-Generation Live Attenuated Vaccines for Porcine Epidemic Diarrhea Using Reverse Genetic Techniques

Highs and Lows in Calicivirus Reverse Genetics

Progress of research on coronaviruses and toroviruses in large domestic animals using reverse genetics systems

Contact Info

Product

Resources

About