Reverse genetics approaches: a novel strategy for African horse sickness virus vaccine design

Calvo-Pinilla, Eva; Marín-López, Alejandro; Utrilla-Trigo, Sergio; Jiménez-Cabello, Luís; Ortego, Javier

doi:10.1016/j.coviro.2020.06.003

Cited by 10 publications

(10 citation statements)

References 65 publications

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“…Orbiviruses, such as BTV and AHSV, cause serious damage to animal health and have a significant economic impact on society [ 9 , 23 , 26 ]. Currently, the best approach to prevent BTV and AHSV infections is vaccination, although this approach presents multiple limitations, and vaccine strategies need to be improved [ 12 , 26 , 27 , 28 , 29 , 30 , 31 ]. More importantly, although vaccines can prevent infections creating protective immunity, they are not an effective method to treat infected animals, which usually need to be sacrificed causing important economic losses.…”

Section: Discussionmentioning

confidence: 99%

“…Vaccination is considered the most effective approach to protect against BTV or AHSV infections [ 12 , 26 , 27 , 28 , 29 , 30 , 31 ]. However, the control of these viral infections is a difficult task due to uncontrolled vector propagation, the number of circulating serotypes in combination with the lack of cross-protection between them, and the wide host range [ 5 , 6 , 7 , 8 , 9 , 25 , 32 ].…”

Section: Introductionmentioning

confidence: 99%

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Inhibition of Orbivirus Replication by Aurintricarboxylic Acid

Alonso

Utrilla-Trigo

Calvo-Pinilla

et al. 2020

IJMS

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Bluetongue virus (BTV) and African horse sickness virus (AHSV) are vector-borne viruses belonging to the Orbivirus genus, which are transmitted between hosts primarily by biting midges of the genus Culicoides. With recent BTV and AHSV outbreaks causing epidemics and important economy losses, there is a pressing need for efficacious drugs to treat and control the spread of these infections. The polyanionic aromatic compound aurintricarboxylic acid (ATA) has been shown to have a broad-spectrum antiviral activity. Here, we evaluated ATA as a potential antiviral compound against Orbivirus infections in both mammalian and insect cells. Notably, ATA was able to prevent the replication of BTV and AHSV in both cell types in a time- and concentration-dependent manner. In addition, we evaluated the effect of ATA in vivo using a mouse model of infection. ATA did not protect mice against a lethal challenge with BTV or AHSV, most probably due to the in vivo effect of ATA on immune system regulation. Overall, these results demonstrate that ATA has inhibitory activity against Orbivirus replication in vitro, but further in vivo analysis will be required before considering it as a potential therapy for future clinical evaluation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Inhibition of Orbivirus Replication by Aurintricarboxylic Acid

Alonso

Utrilla-Trigo

Calvo-Pinilla

et al. 2020

IJMS

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“…As a result, there has been recent progress on the development of recombinant and inactivated vaccines, protein, and virus-like particles [10,[38][39][40][41][42]. Reverse genetics systems and recombinant techniques are being used to develop new vaccines to target specific antigens of the AHSV, including several viral and nonstructural proteins of the virus, including the capsid proteins VP2 and VP5, as well as NS1, which may elicit an interferon gamma host antiviral response [10][11][12]16,43]. These newer technologies aim to create a DIVA vaccine, which will greatly improve the control and detection of the virus.…”

Section: African Horse Sicknessmentioning

confidence: 99%

Transboundary Animal Diseases, an Overview of 17 Diseases with Potential for Global Spread and Serious Consequences

Clemmons

Alfson

Dutton

2021

Animals

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Animals provide food and other critical resources to most of the global population. As such, diseases of animals can cause dire consequences, especially disease with high rates of morbidity or mortality. Transboundary animal diseases (TADs) are highly contagious or transmissible, epidemic diseases, with the potential to spread rapidly across the globe and the potential to cause substantial socioeconomic and public health consequences. Transboundary animal diseases can threaten the global food supply, reduce the availability of non-food animal products, or cause the loss of human productivity or life. Further, TADs result in socioeconomic consequences from costs of control or preventative measures, and from trade restrictions. A greater understanding of the transmission, spread, and pathogenesis of these diseases is required. Further work is also needed to improve the efficacy and cost of both diagnostics and vaccines. This review aims to give a broad overview of 17 TADs, providing researchers and veterinarians with a current, succinct resource of salient details regarding these significant diseases. For each disease, we provide a synopsis of the disease and its status, species and geographic areas affected, a summary of in vitro or in vivo research models, and when available, information regarding prevention or treatment.

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“…In light of the absence of therapeutic treatments [ 14 , 15 , 16 ], vaccination is the most effective prophylactic measure against these three relevant ruminant diseases [ 17 , 18 , 19 , 20 ]. Classic vaccine approaches (live attenuated and inactivated vaccines) have contributed to the control of these viral diseases.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, novel approaches have emerged by the genetic modification of orbiviruses. The development of reverse genetics systems allowed the production of disabled infectious single cycle (DISC) and disabled infectious single animal (DISA) vaccines [ 18 , 19 ], showing promising results in BTV and AHSV natural hosts [ 18 ]. Viral vector and subunit vaccines have also been shown to be efficacious in natural hosts [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Nanoparticle- and Microparticle-Based Vaccines against Orbiviruses of Veterinary Importance

et al. 2022

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Bluetongue virus (BTV) and African horse sickness virus (AHSV) are widespread arboviruses that cause important economic losses in the livestock and equine industries, respectively. In addition to these, another arthropod-transmitted orbivirus known as epizootic hemorrhagic disease virus (EHDV) entails a major threat as there is a conducive landscape that nurtures its emergence in non-endemic countries. To date, only vaccinations with live attenuated or inactivated vaccines permit the control of these three viral diseases, although important drawbacks, e.g., low safety profile and effectiveness, and lack of DIVA (differentiation of infected from vaccinated animals) properties, constrain their usage as prophylactic measures. Moreover, a substantial number of serotypes of BTV, AHSV and EHDV have been described, with poor induction of cross-protective immune responses among serotypes. In the context of next-generation vaccine development, antigen delivery systems based on nano- or microparticles have gathered significant attention during the last few decades. A diversity of technologies, such as virus-like particles or self-assembled protein complexes, have been implemented for vaccine design against these viruses. In this work, we offer a comprehensive review of the nano- and microparticulated vaccine candidates against these three relevant orbiviruses. Additionally, we also review an innovative technology for antigen delivery based on the avian reovirus nonstructural protein muNS and we explore the prospective functionality of the nonstructural protein NS1 nanotubules as a BTV-based delivery platform.

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Reverse genetics approaches: a novel strategy for African horse sickness virus vaccine design

Cited by 10 publications

References 65 publications

Inhibition of Orbivirus Replication by Aurintricarboxylic Acid

Inhibition of Orbivirus Replication by Aurintricarboxylic Acid

Transboundary Animal Diseases, an Overview of 17 Diseases with Potential for Global Spread and Serious Consequences

Nanoparticle- and Microparticle-Based Vaccines against Orbiviruses of Veterinary Importance

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