Reverse-flow diafiltration for continuous in situ product recovery

Carstensen, Frederike; Marx, Christian; André, João; Melin, Thomas; Weßling, Matthias

doi:10.1016/j.memsci.2012.06.034

Cited by 18 publications

(17 citation statements)

References 26 publications

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“…Up to now, RFD has only been described for microbial culture processes . However, recombinant protein production in mammalian cell lines could also be efficiently carried out using RFD.…”

Section: Resultsmentioning

confidence: 99%

“…The most recent studies in the literature often apply the ATF module (Alternating tangential flow technology), which is commercially available in several scales . Recently, reverse‐flow diafiltration (RFD) was introduced as an internal or so called in situ product recovery and cell retention process for continuous and perfusion cultures . RFD alternates harvesting of product with feeding of fresh medium over the same submerged membrane, thus enabling gentle cell retention while the formation of a fouling layer is minimized.…”

Section: Introductionmentioning

confidence: 99%

“…RFD alternates harvesting of product with feeding of fresh medium over the same submerged membrane, thus enabling gentle cell retention while the formation of a fouling layer is minimized. In previous studies, RFD was intensively discussed regarding membrane module and settings . Furthermore, successful application of RFD for the recovery of single‐chain antibodies secreted by yeast, as well as itaconic acid produced by fungi, was shown with regard to long‐term stability and high product transmission …”

Section: Introductionmentioning

confidence: 99%

“…28,29 Recently, reverse-flow diafiltration (RFD) was introduced as an internal or so called in situ product recovery and cell retention process for continuous and perfusion cultures. [30][31][32][33] RFD alternates harvesting of product with feeding of fresh medium over the same submerged membrane, thus enabling gentle cell retention while the formation of a fouling layer is minimized. In previous studies, RFD was intensively discussed regarding membrane module and settings.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

In situ cell retention of a CHO culture by a reverse‐flow diafiltration membrane bioreactor

Meier

Djeljadini

Regestein

et al. 2014

Biotechnology Progress

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Heterogeneities occur in various bioreactor designs including cell retention devices. Whereas in external devices changing environmental conditions cannot be prevented, cells are retained in their optimal environment in internal devices. Conventional reverse-flow diafiltration utilizes an internal membrane device, but pulsed feeding causes temporal heterogeneities. In this study, the influence of conventional reverse-flow diafiltration on the yeast Hansenula polymorpha is investigated. Alternating 180 s of feeding with 360 s of non-feeding at a dilution rate of 0.2 h(-1) results in an oscillating DOT signal with an amplitude of 60%. Thereby, induced short-term oxygen limitations result in the formation of ethanol and a reduced product concentration of 25%. This effect is enforced at increased dilution rate. To overcome this cyclic problem, sequential operation of three membranes is introduced. Thus, quasi-continuous feeding is achieved reducing the oscillation of the DOT signal to an amplitude of 20% and 40% for a dilution rate of 0.2 h(-1) and 0.5 h(-1) , respectively. Fermentation conditions characterized by complete absence of oxygen limitation and without formation of overflow metabolites could be obtained for dilution rates from 0.1 h(-1) - 0.5 h(-1) . Thus, sequential operation of three membranes minimizes oscillations in the DOT signal providing a nearly homogenous culture over time.

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“…Up to now, RFD has only been described for microbial culture processes . However, recombinant protein production in mammalian cell lines could also be efficiently carried out using RFD.…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

In situ cell retention of a CHO culture by a reverse‐flow diafiltration membrane bioreactor

Meier

Djeljadini

Regestein

et al. 2014

Biotechnology Progress

Self Cite

View full text Add to dashboard Cite

show abstract

“…Cells were cultured in the bioreactor, outside the membrane. The membrane module was constructed as described by Carstensen et al [6,13], but modified to allow operation of three membranes in parallel (pumps work simultaneously) or individually (pumps work in a time shifted sequence) [7]. Each membrane had a length of 1 m and was coiled around baffles and fixed by attached hooks.…”

Section: Membrane Bioreactormentioning

confidence: 99%

Quasi-continuous fermentation in a reverse-flow diafiltration bioreactor

Meier

Carstensen

Weßling

et al. 2014

Biochemical Engineering Journal

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In situ product recovery techniques aiming to obtain biotechnological products: A glance to current knowledge

Santos

Albuquerque

Ribeiro

et al. 2020

Biotech and App Biochem

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Biotechnology and bioengineering techniques have been widely used in the production of biofuels, chemicals, pharmaceuticals, and food additives, being considered a “green” form of production because they use renewable and nonpolluting energy sources. On the other hand, in the traditional processes of production, the target product obtained by biotechnological routes must undergo several stages of purification, which makes these processes more expensive. In the past few years, some works have focused on processes that integrate fermentation to the recovery and purification steps necessary to obtain the final product required. This type of process is called in situ product recovery or extractive fermentation. However, there are some differences in the concepts of the techniques used in these bioprocesses. In this way, this review sought to compile relevant content on considerations and procedures that are being used in this field, such as evaporation, liquid–liquid extraction, permeation, and adsorption techniques. Also, the objective of this review was to approach the different configurations in the recent literature of the processes employed and the main bioproducts obtained, which can be used in the food, pharmaceutical, chemical, and/or fuel additives industry. We intended to elucidate concepts of these techniques, considered very recent, but which emerge as a promising alternative for the integration of bioprocesses.

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Reverse-flow diafiltration for continuous in situ product recovery

Cited by 18 publications

References 26 publications

In situ cell retention of a CHO culture by a reverse‐flow diafiltration membrane bioreactor

In situ cell retention of a CHO culture by a reverse‐flow diafiltration membrane bioreactor

Quasi-continuous fermentation in a reverse-flow diafiltration bioreactor

In situ product recovery techniques aiming to obtain biotechnological products: A glance to current knowledge

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