Reverse engineering approach: a step towards a new era of vaccinology with special reference to <i>Salmonella</i>

Vij, Shania; Thakur, Reena; Rishi, Praveen

doi:10.1080/14760584.2022.2148661

Cited by 5 publications

(4 citation statements)

References 183 publications

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“…Further, immunoinformatic studies indicate that efflux pump systems such as RND, MFS, MATE, SMR, and ABC are potential adjuvants comprising B cell and T cell epitopes, which have a strong binding affinity to HLA alleles, stable response with TLR2 immune receptor, and high antigenicity. This demonstrates the role of the efflux system of Salmonella in the progression of therapeutic applications. , There are several methods to understand antibiotic intake and uptake by OM and efflux systems to study the pharmacokinetics of drugs, which helps to formulate structurally enhanced molecules with improved access to reduce the emergence of MDR …”

Section: Application Of Omps and Efflux Pumps For Possible Therapeuti...mentioning

confidence: 99%

“…This demonstrates the role of the efflux system of Salmonella in the progression of therapeutic applications. 154,155 There are several methods to understand antibiotic intake and uptake by OM and efflux systems to study the pharmacokinetics of drugs, which helps to formulate structurally enhanced molecules with improved access to reduce the emergence of MDR. 156 Since efflux plays a crucial role in the development of antibiotic resistance, removing these resistance-causing factors could enhance the effects of substrate antibiotics.…”

Section: ■ Application Of Omps and Efflux Pumps For Possible Therapeu...mentioning

confidence: 99%

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Outer Membrane Proteins and Efflux Pumps Mediated Multi-Drug Resistance in Salmonella: Rising Threat to Antimicrobial Therapy

Akshay,

Deekshit,

Mohan Raj

et al. 2023

ACS Infect. Dis.

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Despite colossal achievements in antibiotic therapy in recent decades, drug-resistant pathogens have remained a leading cause of death and economic loss globally. One such WHO-critical group pathogen is Salmonella. The extensive and inappropriate treatments for Salmonella infections have led from multi-drug resistance (MDR) to extensive drug resistance (XDR). The synergy between efflux-mediated systems and outer membrane proteins (OMPs) may favor MDR in Salmonella. Differential expression of the efflux system and OMPs (influx) and positional mutations are the factors that can be correlated to the development of drug resistance. Insights into the mechanism of influx and efflux of antibiotics can aid in developing a structurally stable molecule that can be proficient at escaping from the resistance loops in Salmonella. Understanding the strategic responsibilities and developing policies to address the surge of drug resistance at the national, regional, and global levels are the needs of the hour. In this Review, we attempt to aggregate all the available research findings and delineate the resistance mechanisms by dissecting the involvement of OMPs and efflux systems. Integrating major OMPs and the efflux system’s differential expression and positional mutation in Salmonella may provide insight into developing strategic therapies for one health application.

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Section: Application Of Omps and Efflux Pumps For Possible Therapeuti...mentioning

confidence: 99%

Section: ■ Application Of Omps and Efflux Pumps For Possible Therapeu...mentioning

confidence: 99%

Outer Membrane Proteins and Efflux Pumps Mediated Multi-Drug Resistance in Salmonella: Rising Threat to Antimicrobial Therapy

Akshay,

Deekshit,

Mohan Raj

et al. 2023

ACS Infect. Dis.

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show abstract

“…With the proliferation of big genomic and proteomic data and computing power, a successful vaccine could be developed by accessing vast information about the virus structure, protein structures, and predicted epitopes. A combination of computational methods and immunoinformatics had formed the bases for the development of multi-epitopes vaccines 18 , 19 . In reverse vaccinology, which analyzes a pathogen's genome and identifies its prospective antigenic proteins, multi-epitope vaccines are produced in a shorter period of time and at a lower cost, with safety and highest therapeutic efficiency and minimal adverse reactions 18 , 19 .…”

Section: Introductionmentioning

confidence: 99%

“…A combination of computational methods and immunoinformatics had formed the bases for the development of multi-epitopes vaccines 18 , 19 . In reverse vaccinology, which analyzes a pathogen's genome and identifies its prospective antigenic proteins, multi-epitope vaccines are produced in a shorter period of time and at a lower cost, with safety and highest therapeutic efficiency and minimal adverse reactions 18 , 19 . In addition, to the boosting of immunogenicity, vaccines with multi-epitopes may improve immune responses because they contain epitopes representative of different target genes 20 .…”

Section: Introductionmentioning

confidence: 99%

Immunoinformatics, molecular docking and dynamics simulation approaches unveil a multi epitope-based potent peptide vaccine candidate against avian leukosis virus

Elshafei,

Mahmoud,

Almofti

2024

Sci Rep

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Lymphoid leukosis is a poultry neoplastic disease caused by avian leukosis virus (ALV) and is characterized by high morbidity and variable mortality rates in chicks. Currently, no effective treatment and vaccination is the only means to control it. This study exploited the immunoinformatics approaches to construct multi-epitope vaccine against ALV. ABCpred and IEDB servers were used to predict B and T lymphocytes epitopes from the viral proteins, respectively. Antigenicity, allergenicity and toxicity of the epitopes were assessed and used to construct the vaccine with suitable adjuvant and linkers. Secondary and tertiary structures of the vaccine were predicted, refined and validated. Structural errors, solubility, stability, immune simulation, dynamic simulation, docking and in silico cloning were also evaluated.The constructed vaccine was hydrophilic, antigenic and non-allergenic. Ramchandran plot showed most of the residues in the favored and additional allowed regions. ProsA server showed no errors in the vaccine structure. Immune simulation showed significant immunoglobulins and cytokines levels. Stability was enhanced by disulfide engineering and molecular dynamic simulation. Docking of the vaccine with chicken’s TLR7 revealed competent binding energies.The vaccine was cloned in pET-30a(+) vector and efficiently expressed in Escherichia coli. This study provided a potent peptide vaccine that could assist in tailoring a rapid and cost-effective vaccine that helps to combat ALV. However, experimental validation is required to assess the vaccine efficiency.

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Dual immunization with CdtB protein and flagellin epitope offers augmented protection against enteric fever in mice

Vij,

Thakur,

Singh

et al. 2023

Life Sciences

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Reverse engineering approach: a step towards a new era of vaccinology with special reference to Salmonella

Cited by 5 publications

References 183 publications

Outer Membrane Proteins and Efflux Pumps Mediated Multi-Drug Resistance in Salmonella: Rising Threat to Antimicrobial Therapy

Outer Membrane Proteins and Efflux Pumps Mediated Multi-Drug Resistance in Salmonella: Rising Threat to Antimicrobial Therapy

Immunoinformatics, molecular docking and dynamics simulation approaches unveil a multi epitope-based potent peptide vaccine candidate against avian leukosis virus

Dual immunization with CdtB protein and flagellin epitope offers augmented protection against enteric fever in mice

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