Abstract:Energy dependent reverse electron flow reactions in isolated thylakoids provide a unique tool to study, in the dark, the coupling between the ATP synthase, proton transport and the electron transfer system. Appropriate experimental conditions have been established to follow experimentally the following reactions: 1. ATP driven proton uptake into the inner-thylakoid space, which requires preactivation of the ATP synthase. 2. ATP driven reverse electron transport, which involves proton transport as an intermedia… Show more
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