Abstract:Objective. Diabetic nephropathy (DN) is a common microvascular complication of diabetes mellitus, and insulin therapy has many side effects in the treatment of DN. Islet transplantation has emerged as a promising therapy for diabetic patients. This study was established to investigate its advantageous effects in a rat model of early DN. Methods. Streptozotocin was administered to the rats to induce diabetes. Twelve weeks later, the diabetic rats were divided into 3 groups: the islet-transplanted group (IT grou… Show more
“…Figure 2Meta-analysis of the studies assessing glomerular volume: A) studies with different times of exposure to diabetes (two or four weeks); B) studies with equal times of exposure to diabetes (two weeks). Mean values, standard deviations, differences between mean values, and 95% confidence interval were raised to 10 6…”
Section: Resultsmentioning
confidence: 99%
“…, 6 Structural changes include glomerular and renal hypertrophy, thickening of the basement membrane, tubular atrophy, and interstitial fibrosis, which in turn foster the development of hyperfiltration, proteinuria, and decreased renal function 26. Screening for DKD includes annual urine albumin and glomerular filtration rate (GFR) tests 7…”
The results of the studies included in the systematic review diverged. However, the meta-analysis showed that the animals given C-peptide had lower glomerular volumes and lower urine potassium levels.
“…Figure 2Meta-analysis of the studies assessing glomerular volume: A) studies with different times of exposure to diabetes (two or four weeks); B) studies with equal times of exposure to diabetes (two weeks). Mean values, standard deviations, differences between mean values, and 95% confidence interval were raised to 10 6…”
Section: Resultsmentioning
confidence: 99%
“…, 6 Structural changes include glomerular and renal hypertrophy, thickening of the basement membrane, tubular atrophy, and interstitial fibrosis, which in turn foster the development of hyperfiltration, proteinuria, and decreased renal function 26. Screening for DKD includes annual urine albumin and glomerular filtration rate (GFR) tests 7…”
The results of the studies included in the systematic review diverged. However, the meta-analysis showed that the animals given C-peptide had lower glomerular volumes and lower urine potassium levels.
“…In addition, recipients can obtain additional donor islet cells through repeated surgeries,to fully restore blood glucose levels in diabetic patients to normal levels [ 13 , 14 ]. Recent studies have shown that pancreas or islet transplantation can ameliorate and even reverse diabetic complications, including nephropathy, retinopathy and neuropathy in the early stage [ 15 – 17 ]. However, the effect of islet transplantation on DMED has not been reported.…”
BackgroundGlycaemic control is one of the most effective strategies for the treatment of diabetes-related erectile dysfunction (DMED). Compared to conventional anti-diabetic drugs and insulin, islet transplantation is more effective in the treatment of diabetic complications. The aim of this study was to investigate the efficacy of islet transplantation for reversing advanced-stage DMED in rats and to observe its influence on corpus cavernosum fibrosis.MethodsWistar rats were intraperitoneally injected with streptozotocin to establish a diabetes model. After 12 weeks, the rats were divided into 4 groups: diabetic, insulin, islet transplantation, and normal control. Following supplementation, the changes in blood glucose and weight were determined sequentially. Penile erectile function was evaluated by apomorphine experiments in the fourth week, and the penile corpus cavernosum was also collected for assessment by Masson staining, immunohistochemistry and Western blot to observe the spongy tissue and the related cellular changes at the molecular level.ResultsIslet transplantation significantly ameliorated penile erectile function in advanced-stage diabetic rats. The ratio of corpus cavernosum smooth muscle cells to fibroblasts and the expression level of α-SMA in the islet transplantation group were significantly higher than those in the diabetic and insulin groups. In addition, the expression levels of TGF-β1, p-Samd2, and connective tissue growth factor (CTGF) in the islet transplantation and insulin groups were much lower than those in the diabetic group, while those in the islet transplantation group were significantly lower than those in the insulin group.ConclusionsOur findings strongly suggest that islet transplantation can promote the regeneration of smooth muscle cells and ameliorate corpus cavernosum fibrosis to restore its normal structure in advanced-stage diabetic rats. The possible mechanism of ameliorating corpus cavernosum fibrosis by islet transplantation may be associated with improvement of the hyperglycaemic status in diabetic rats, thereby inhibiting the TGF-β1/Samd2/CTGF pathway.
“…More importantly, islet transplantation can effectively prevent and reverse diabetes-induced microvascular diseases, such as diabetic retinopathy and nephropathy [ 5 , 6 ]. Our previous work found that in a rat model of early diabetic nephropathy, islet transplantation could stop and reverse the progression of diabetic nephropathy [ 7 ].…”
Diabetic nephropathy (DN) is a serious microvascular complication of diabetes. Compared with other therapies for diabetic patients, islet transplantation can effectively prevent and reverse diabetes-induced microvascular disease, such as diabetic retinopathy and nephropathy. PTX3 is the only long pentraxin that can be detected in renal tissue. In this study, we investigated the expression of PTX3 when early DN was reversed after islet transplantation. Methods. Diabetes was induced in rats by injecting streptozotocin (STZ). Twelve weeks later, the diabetic rats were divided into 2 groups: the islet transplantation group (IT) and the diabetic nephropathy group (DN). Renal injury, renal function, and the expression of PTX3 in the plasma and the kidneys were assessed with urinalysis, immunohistochemical staining, and Western blot, respectively. Results. The expression of PTX3 in the kidney was significantly decreased in the DN group but increased in the IT group because of the reversal of DN. Conclusions. Our data showed that the level of PTX3 in renal tissue is closely related to renal injury in DN. This may be used to quantify the extent of renal injury in DN, provide a potential early indicator of renal tubular injury in early DN patients, and assess DN clinical progression.
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