Reversal of drug-induced gingival overgrowth by UV-mediated apoptosis of gingival fibroblasts — an in vitro study.

Ritchhart, Casey; Joy, Anita

doi:10.1016/j.aanat.2018.01.001

Cited by 11 publications

(11 citation statements)

References 29 publications

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“…La identificación de la calnexina, al igual que TOMM20 y LAMP1, indica que la ausencia de SFB no afectaría el funcionamiento de estas organelas celulares, permitiendo avalar la utilización de los fibroblastos gingivales, como modelo de estudios in vitro, en presencia y/o ausencia de SFB. Diversos estudios, donde se desean conservar los aspectos fisiológicos de los fibroblastos gingivales, han optado por la utilización de SFB a 10%, concentración similar a la llevada a cabo en el presente estudio (Soares et al 2018; Ritchhart & Joy, 2018); sin embargo, con el fin de utilizar los fibroblastos gingivales, como modelos en el desarrollo de diversos procesos patológicos, tales como la mineralización ectópica, muchos estudios optan por disminuir o suprimir la presencia de SFB (Ritchhart & Joy, 2018;Takeuchi et al 2017;Basso et al 2017).…”

Section: Resultados Y Discusiónunclassified

Efecto de la ausencia de suero fetal bovino sobre la morfología y organelas de células fibroblásticas

Simancas-Escorcia

Caballero

2020

Rev. U.D.C.A Act. & Div. Cient.

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Los fibroblastos son células constituyentes de los tejidos conectivo. Los fibroblastos gingivales (FGs), células responsables de la síntesis de la matriz extracelular (MEC) en el tejido conectivo gingival, participan en la regulación de los procesos de cicatrización y de reparación de la encía. Debido a su potencial regenerativo, los FGs podrían ser células capaces de contribuir a mejorar los procesos de cicatrización, a nivel local y sistémico e, incluso, ser utilizadas como un modelo celular útil en la comprensión de los aspectos fisiopatológicos de la cavidad oral. El objetivo del presente trabajo fue describir el impacto de la concentración del suero fetal bovino (SFB), en la supervivencia, el crecimiento y la expresión de marcadores celulares en los FGs. Cultivos celulares de FGs fueron realizados durante 7 días, utilizando medio de cultivo DMEM (Dulbecco’s Modified Eagle’s médium), en ausencia y presencia de 10% de SFB. Análisis morfológicos e inmunohistoquímicos de la actina, mitocondrias, lisosomas y retículo endoplasmático (RE) fueron usados para evaluar el impacto de la concentración del SFB sobre los FGs. Los resultados indican que los FGs cultivados en presencia de 10% de SFB tuvieron una tasa de crecimiento más elevada en comparación con los FGs, cultivados en ausencia de SFB. El marcaje de los elementos celulares indica la ausencia de alteraciones en las organelas celulares de los FGs, cuando son cultivados en ausencia de SFB. En conclusión, los FGs son capaces de sobrevivir, proliferar y conservar sus características morfológicas, cuando son cultivados en presencia y ausencia de SFB.

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Section: Resultados Y Discusiónunclassified

Efecto de la ausencia de suero fetal bovino sobre la morfología y organelas de células fibroblásticas

Simancas-Escorcia

Caballero

2020

Rev. U.D.C.A Act. & Div. Cient.

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“…Recently, the UV phototreatment (UV radiation of –254 nm) has been proposed by Ritchhart and Joy [31]. It might be a viable nonsurgical treatment modality as it is based on the activation of fibroblast cell apoptosis [31].…”

Section: Discussionmentioning

confidence: 99%

Periodontal Management of Cyclosporin A-Induced Gingival Overgrowth: A Nonsurgical Approach

Malek

Houari

Kissa

2019

Case Reports in Dentistry

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Gingival overgrowth is a major and frequent unwanted effect accompanying the chronic usage of antihypertensive, anticonvulsant, and immunosuppressant drugs. The expression and the severity of this tissue-specific condition are influenced by a variety of factors, mainly drug and periodontal variables. Such increased volume of gingiva may compromise normal oral functions, aesthetics in addition to the patients’ ability to practice optimal oral hygiene. The management of gingival overgrowth includes nonsurgical approach, surgical approach, or both of them for severe cases of gingival overgrowth as well as drug withdrawal. This case report illustrates a successful nonsurgical management of a 21-year-old patient with cyclosporin A-induced gingival overgrowth who experienced a total regression of the gingival enlargement without any surgical procedure or drug substitution. And it highlights therefore the key role of supportive periodontal therapy in maintaining good and stable outcomes over 2 years of follow-up.

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“…5 Narwal et al, 24 showed in their case report an abnormal unilateral palatal soft tissue enlargement in a 61-year-old hypertensive female using amlodipine, which is a safe antihypertensive drug. Ritchhart et al, 25 stated a process behind uncontrolled cell proliferation in druginduced gingival overgrowth which is the suppression of apoptotic pathways, with the following effect on the normal gingival formation by cell turnover. Hormonal differences which take place in pregnancy and puberty, in addition to the use of oral contraceptives have been associated with gingival overgrowth.…”

Section: Conditions Causing Gingival Enlargementmentioning

confidence: 99%

“…5 Other effects such as age, demographic and pharmacokinetic changes, genetic predisposition, oral hygiene condition, as well as molecular and cellular variables may impact the mechanism of gingival enlargement. 25,26 Orthodontic treatment using orthodontic appliances can also participate in the presence of gingival enlargement. 27 In the case of leukemia, leukemic cells might infiltrate to the gingiva, 28 and this will cause some manifestations to be present such as gingival enlargement and bleeding.…”

Section: Conditions Causing Gingival Enlargementmentioning

confidence: 99%

Gummy Smile: A Review of Etiology, Manifestations, and Treatment

Mahardawi

Chaisamut

Wongsirichat

2019

Smj

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Excessive gingival display, or what is known as Gummy Smile, is a clinical condition which is attracting a great attention and focus recently. Having knowledge and background about the cause and the treatment of each case of excessive gingival display is essential for making the accurate diagnosis and performing the correct procedure. This article reviews the most common causes of excessive gingival display, manifestations of this condition, and some of the important treatment options for Gummy Smile. In addition, the article also puts the light on some points regarding the accepted measurement in several studies, and considers the prediction planning for Gummy Smile associated with a skeletal etiology.

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Reversal of drug-induced gingival overgrowth by UV-mediated apoptosis of gingival fibroblasts — an in vitro study.

Cited by 11 publications

References 29 publications

Efecto de la ausencia de suero fetal bovino sobre la morfología y organelas de células fibroblásticas

Efecto de la ausencia de suero fetal bovino sobre la morfología y organelas de células fibroblásticas

Periodontal Management of Cyclosporin A-Induced Gingival Overgrowth: A Nonsurgical Approach

Gummy Smile: A Review of Etiology, Manifestations, and Treatment

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