Reveromycins A and B from Streptomyces sp. 3–10: Antifungal Activity against Plant Pathogenic Fungi In vitro and in a Strawberry Food Model System

Lyu, Ang; Líu, Hao; Che, Hongjie; Yang, Long; Zhang, Jing; Wu, Mingde; Chen, Weidong; Li, Guoqing

doi:10.3389/fmicb.2017.00550

Cited by 48 publications

(41 citation statements)

References 37 publications

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“…Two compounds were purified from Streptomyces sp. 3-10, and were identified as reveromycins A and B, which demonstrated high antifungal activity against Botrytis cinerea, Mucor hiemalis, Rhizopus stolonifer , and Sclerotinia sclerotiorum (Lyu et al, 2017 ). Srivastava et al found Chrestomyceticus could produce metabolites with antifungal activity against Candida albicans (Srivastava and Dubey, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

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Growth Promotion and Disease Suppression Ability of a Streptomyces sp. CB-75 from Banana Rhizosphere Soil

et al. 2018

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An actinomycete strain, CB-75, was isolated from the soil of a diseased banana plantation in Hainan, China. Based on phenotypic and molecular characteristics, and 99.93% sequence similarity with Streptomyces spectabilis NBRC 13424 (AB184393), the strain was identified as Streptomyces sp. This strain exhibited broad-spectrum antifungal activity against 11 plant pathogenic fungi. Type I polyketide synthase (PKS-I) and non-ribosomal peptide synthetase (NRPS) were detected, which were indicative of the antifungal compounds that Streptomyces sp. CB-75 could produce. An ethyl acetate extract from the strain exhibited the lowest minimum inhibitory concentration (MIC) against Colletotrichum musae (ATCC 96167) (0.78 μg/ml) and yielded the highest antifungal activity against Colletotrichum gloeosporioides (ATCC 16330) (50.0 μg/ml). Also, spore germination was significantly inhibited by the crude extract. After treatment with the crude extract of Streptomyces sp. CB-75 at the concentration 2 × MIC, the pathogenic fungi showed deformation, shrinkage, collapse, and tortuosity when observed by scanning electron microscopy (SEM). By gas chromatography-mass spectrometry (GC-MS) of the crude extract, 18 chemical constituents were identified; (Z)-13-docosenamide was the major constituent. Pot experiments showed that the incidence of banana seedlings was reduced after using Streptomyces sp. CB-75 treatment. The disease index was 10.23, and the prevention and control effect was 83.12%. Furthermore, Streptomyces sp. CB-75 had a growth-promoting effect on banana plants. The chlorophyll content showed 88.24% improvement, the leaf area, root length, root diameter, plant height, and stem showed 88.24, 90.49, 136.17, 61.78, and 50.98% improvement, respectively, and the shoot fresh weight, root fresh weight, shoot dry weight, and root dry weight showed 82.38, 72.01, 195.33, and 113.33% improvement, respectively, compared with treatment of fermentation broth without Streptomyces sp. CB-75. Thus, Streptomyces sp. CB-75 is an important microbial resource as a biological control against plant pathogenic fungi and for promoting banana growth.

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Section: Discussionmentioning

confidence: 99%

“…(Ser et al, 2016 ). Streptomyces species are Gram-positive, filamentous, and sporulating actinobacteria, with a high G + C content in their genomes (Lyu et al, 2017 ). They exhibit an immense biocontrol activity against a range of phytopathogens (Wang X. N. et al, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Growth Promotion and Disease Suppression Ability of a Streptomyces sp. CB-75 from Banana Rhizosphere Soil

et al. 2018

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“…Many Streptomyces species have been successfully evaluated as biocontrol agents against phytopathogens ( Berg et al, 2001 ; Minuto et al, 2006 ; Law et al, 2017 ). Some antibiotic compounds ( Al-Bari et al, 2007 ; Zucchi et al, 2008 ; Lyu et al, 2017 ) and other compounds such as chitinases ( Gomes et al, 2001 ; Mander et al, 2016 ) produced by Streptomyces spp. have a strong antagonistic effect on growth and development of Aspergillus .…”

Section: Introductionmentioning

confidence: 99%

Biocontrol of Aspergillus flavus on Peanut Kernels Using Streptomyces yanglinensis 3-10

et al. 2018

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The bacterium, Streptomyces yanglinensis 3-10, shows promise in the control of many phytopathogenic fungi. In this study, S. yanglinensis and its antifungal substances, culture filtrate (CF3-10) and crude extracts (CE3-10), were evaluated for their activity in reducing growth and aflatoxin AFB1 production by Aspergillus flavus, both in vitro and in vivo on peanut kernels. The results showed that in dual culture conditions, S. yanglinensis reduced the mycelial growth of A. flavus about 41% as compared to control. The mycelial growth of A. flavus was completely inhibited on potato dextrose agar amended with CF3-10 at 3% (v/v) or CE3-10 at 2.5 μg/ml. In liquid culture experiments, growth inhibition ranged from 32.3 to 91.9% with reduction in AFB1 production ranging from 46.4 to 93.4% using different concentrations of CF3-10 or CE3-10. For in vivo assays, CF3-10 at 0.133 ml/g (v/w) or CE3-10 at 13.3 μg/g (w/w) reduced the postharvest decay of peanut kernels by inhibiting visible growth of A. flavus leading to an 89.4 or 88.1% reduction in AFB1 detected, respectively. Compared with the controls, CF3-10 and CE3-10 in A. flavus shake culture significantly reduced expression levels of two AFB1 biosynthesis genes, aflR and aflS. Furthermore, electron microscopy observation showed that CF3-10 (2%, v/v) caused hyphae growth to be abnormal and shriveled, cell organelles to degenerate and collapse, large vacuoles to appear. These results suggest that S. yanglinensis 3-10 has potential as an alternative to chemical fungicides in protecting peanut kernels and other agricultural commodities against postharvest decay from A. flavus.

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“…Low antifungal activity was observed for the Nystatin. Previous studies also show differential activities of various antifungals against the fungal isolates recovered from various foods [23,30].…”

Section: Discussionmentioning

confidence: 88%

Microbiological Safety and Antibiogram Analysis of Selected Food Products Obtained in the Marketplace of Peshawar and Mardan, KPK, Pakistan

Khan¹,

Ullah²,

Salman³

et al. 2019

Pol. J. Environ. Stud.

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Foodborne diseases are very frequent and can easily transmit from contaminated food and food handlers. Among the foodborne pathogens, strains of Staphylococcus aureus, Bacillus cereus, Listeria monocytogen and Clostridium are very important because of its presence in a wide range of foodstuffs. A total of 520 food samples were collected at Peshawar (50%) and Mardan (50%), Khyber Pakhtunkhwa, Pakistan, from April 2016 to March 2017. Isolates were cultured and discriminated by Gram stain, followed by biochemical identification, disk diffusion assay was performed using antibacterial and antifungal agents. Out of 520, only 122 (23.46%) samples were positive for various types of bacterial and fungal pathogens. Gram-positive bacteria was the predominant (70.49%) followed by fungal pathogens (26.23%) and Gram-negative bacterial pathogens (3.27%) among the total positive samples. Prominent bacterial samples were Staphylococcus aureus, Bacillus spp., Clostridium spp., Staphylococcus

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Reveromycins A and B from Streptomyces sp. 3–10: Antifungal Activity against Plant Pathogenic Fungi In vitro and in a Strawberry Food Model System

Cited by 48 publications

References 37 publications

Growth Promotion and Disease Suppression Ability of a Streptomyces sp. CB-75 from Banana Rhizosphere Soil

Growth Promotion and Disease Suppression Ability of a Streptomyces sp. CB-75 from Banana Rhizosphere Soil

Biocontrol of Aspergillus flavus on Peanut Kernels Using Streptomyces yanglinensis 3-10

Microbiological Safety and Antibiogram Analysis of Selected Food Products Obtained in the Marketplace of Peshawar and Mardan, KPK, Pakistan

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