Revealing the dissimilar structure of microbial communities in different WWTPs that treat fish-canning wastewater with different NaCl content

Correa‐Galeote, David; Roibás-Rozas, Alba; Mosquera-Corral, Anuska; Juárez-Jiménez, B.; González‐López, Jesús; Rodelas, B.

doi:10.1016/j.jwpe.2021.102328

Cited by 20 publications

(7 citation statements)

References 106 publications

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“…It seems that this group could be connected to intracellular urea hydrolysis [109], and urea could be used as an energy source and an important substrate [108]. Gemmatimonadota were also abundant in aquaculture wastewater and in soil irrigated with this water [110], an outlet of wastewater generated during nitrocellulose production [111], and wastewater treatment plants with high salinity [112].…”

Section: Other Environmentsmentioning

confidence: 99%

Phylum Gemmatimonadota and Its Role in the Environment

2022

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Bacteria are an important part of every ecosystem that they inhabit on Earth. Environmental microbiologists usually focus on a few dominant bacterial groups, neglecting less abundant ones, which collectively make up most of the microbial diversity. One of such less-studied phyla is Gemmatimonadota. Currently, the phylum contains only six cultured species. However, data from culture-independent studies indicate that members of Gemmatimonadota are common in diverse habitats. They are abundant in soils, where they seem to be frequently associated with plants and the rhizosphere. Moreover, Gemmatimonadota were found in aquatic environments, such as freshwaters, wastewater treatment plants, biofilms, and sediments. An important discovery was the identification of purple bacterial reaction centers and anoxygenic photosynthesis in this phylum, genes for which were likely acquired via horizontal gene transfer. So far, the capacity for anoxygenic photosynthesis has been described for two cultured species: Gemmatimonas phototrophica and Gemmatimonas groenlandica. Moreover, analyses of metagenome-assembled genomes indicate that it is also common in uncultured lineages of Gemmatimonadota. This review summarizes the current knowledge about this understudied bacterial phylum with an emphasis on its environmental distribution.

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Section: Other Environmentsmentioning

confidence: 99%

Phylum Gemmatimonadota and Its Role in the Environment

2022

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“…Finally, 16S rRNA genes of Candidatus Accumulibacter and Candidatus Competibacter were used as a proxy for polyphosphate- (PAOs) and glycogen-accumulating organisms (GAOs), respectively. Primer sequences, reaction mixtures, and cycling conditions are fully described in Correa-Galeote et al [ 35 ].…”

Section: Methodsmentioning

confidence: 99%

“…However, PHAs production from FOG-enriched streams often requires pretreatment operations, which would increase production costs [ 32 ]. Furthermore, fish-canning wastes are characterized by high contents of salt and organic matter, which can affect microbial communities in their composition and robustness [ 33 , 34 , 35 ]. Therefore, an optimization of the process is required.…”

Section: Introductionmentioning

confidence: 99%

Dynamics of PHA-Accumulating Bacterial Communities Fed with Lipid-Rich Liquid Effluents from Fish-Canning Industries

et al. 2022

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The biosynthesis of polyhydroxyalkanoates (PHAs) from industrial wastes by mixed microbial cultures (MMCs) enriched in PHA-accumulating bacteria is a promising technology to replace petroleum-based plastics. However, the populations’ dynamics in the PHA-accumulating MMCs are not well known. Therefore, the main objective of this study was to address the shifts in the size and structure of the bacterial communities in two lab-scale sequencing batch reactors (SBRs) fed with fish-canning effluents and operated under non-saline (SBR-N, 0.5 g NaCl/L) or saline (SBR-S, 10 g NaCl/L) conditions, by using a combination of quantitative PCR and Illumina sequencing of bacterial 16S rRNA genes. A double growth limitation (DGL) strategy, in which nitrogen availability was limited and uncoupled to carbon addition, strongly modulated the relative abundances of the PHA-accumulating bacteria, leading to an increase in the accumulation of PHAs, independently of the saline conditions (average 9.04 wt% and 11.69 wt%, maximum yields 22.03 wt% and 26.33% SBR-N and SBR-S, respectively). On the other hand, no correlations were found among the PHAs accumulation yields and the absolute abundances of total Bacteria, which decreased through time in the SBR-N and did not present statistical differences in the SBR-S. Acinetobacter, Calothrix, Dyella, Flavobacterium, Novosphingobium, Qipengyuania, and Tsukamurella were key PHA-accumulating genera in both SBRs under the DGL strategy, which was revealed as a successful tool to obtain a PHA-enriched MMC using fish-canning effluents.

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“…For the determination of the bacteria able to realize the dissimilatory NO 3 − reduction, a TOPO ® TA plasmid carrying a 269-basepair of the nrfA gene from E. coli strain GY36 was constructed with the aid of the primers nrfAF2aw (5′-CAR TGY CAY GTB GAR TA) and nrfAR1 (5′-TWN GGC ATR TGR CAR TC) described by Welsh et al ( 2014 ). All reaction mixtures were made in a total volume of 25 mL following Correa-Galeote et al ( 2021a ). Primer sequences and PCR conditions are provided in Table SI.1 in the Supplementary Material.…”

Section: Methodsmentioning

confidence: 99%

Effects of sulphur amino acids on the size and structure of microbial communities of aerobic granular sludge bioreactors

et al. 2022

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Granular activated sludge has been described as a promising tool in treating wastewater. However, the effect of high concentrations of sulphur amino acids, cysteine and methionine, in the evolution, development and stability of AGS-SBRs (aerobic granular sludge in sequential batch reactors) and their microbial communities is not well-established. Therefore, this study aimed to evaluate microbial communities' size, structure and dynamics in two AGS-SBRs fed with two different concentrations of amino acids (50 and 100 mg L−1 of both amino acids). In addition, the impact of the higher level of amino acids was also determined under an acclimatization or shock strategy. While N removal efficiency decreased with amino acids, the removal of the organic matter was generally satisfactory. Moreover, the abrupt presence of both amino acids reduced even further the removal performance of N, whereas under progressive adaptation, the removal yield was higher. Besides, excellent removal rates of cysteine and methionine elimination were found, in all stages below 80% of the influent values. Generally considered, the addition of amino acids weakly impacts the microbial communities' total abundances. On the contrary, the presence of amino acids sharply modulated the dominant bacterial structures. Furthermore, the highest amino acid concentration under the shock strategy resulted in a severe change in the structure of the microbial community. Acidovorax, Flavobacterium, Methylophilus, Stenotrophomonas and Thauera stood out as the prominent bacteria to cope with the high presence of cysteine and methionine. Hence, the AGS-SBR technology is valuable for treating influents enriched in sulphur Aa inclusively when a shock strategy was used.

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Revealing the dissimilar structure of microbial communities in different WWTPs that treat fish-canning wastewater with different NaCl content

Cited by 20 publications

References 106 publications

Phylum Gemmatimonadota and Its Role in the Environment

Phylum Gemmatimonadota and Its Role in the Environment

Dynamics of PHA-Accumulating Bacterial Communities Fed with Lipid-Rich Liquid Effluents from Fish-Canning Industries

Effects of sulphur amino acids on the size and structure of microbial communities of aerobic granular sludge bioreactors

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