Revealing the anti-tumoral effect of Algerian Glaucium flavum roots against human cancer cells

Bournine, Lamine; Bensalem, Sihem; Peixoto, Paul; Gonzalez, Arnaud; Maiza-Benabdesselam, Fadila; Bedjou, Fatiha; Wauters, Jean-Noël; Tits, Monique; Frédérich, Michel; Castronovo, Vincenzo; Bellahcène, Akeila

doi:10.1016/j.phymed.2013.06.007

Cited by 19 publications

(11 citation statements)

References 29 publications

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“…Various types of natural agents extracted from plants, including alkaloid compounds with anticancer activity, have recently been identified (20,21). One promising therapeutic candidate is Halofuginone, an orally available synthetic derivative of a quinazolinone alkaloid that has shown preclinical antitumor activity in various models, including human melanoma bone metastases (18,22).…”

Section: Discussionmentioning

confidence: 99%

Halofuginone induces the apoptosis of breast cancer cells and inhibits migration via downregulation of matrix metalloproteinase-9

Jin

Park

Kim

et al. 2013

International Journal of Oncology

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Abstract. Halofuginone (HF) is extracted from Dichroa febrifuga, a plant used in traditional medicine. We report that the HF extract inhibits the growth of breast cancer cells and induces the generation of reactive oxygen species (ROS) and apoptosis, an important feature of potential anticancer agents. In addition, HF significantly reduces the migration and invasion of MCF-7 and MDA-MB-231 human breast cancer cells after 12-O-tetraecanoylphorbol-13-acetate (TPA) stimulation. As matrix metalloproteinase-9 plays a critical role in tumor metastasis, we analyzed its expression with the HF extract treatment. Western blot analysis and gelatin zymography showed that HF suppresses MMP-9 expression and activity concentrationdependently. HF also decreases the nuclear protein levels of nuclear factor kappa B (NF-κB) and c-fos (AP-1), critical transcription factors regulating MMP-9 expression through binding the MMP-9 promoter region. Luciferase assays showed that HF decreases TPA-induced MMP-9 promoter binding activities of NF-κB and AP-1. Taken together, these are the first results indicating that halofuginone may represent a promising new agent for breast cancer chemotherapy.

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Section: Discussionmentioning

confidence: 99%

Halofuginone induces the apoptosis of breast cancer cells and inhibits migration via downregulation of matrix metalloproteinase-9

Jin

Park

Kim

et al. 2013

International Journal of Oncology

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“…Spectral data from all peaks were recorded in the range of 200–400 nm, and chromatograms were recorded at 290 nm. Protopine was isolated and identified as described previously [10]. The Agilent ChemStation (vA09.03) and DataAnalysis (v5.3) software were used for the acquisition and analysis of chromatographic data.…”

Section: Methodsmentioning

confidence: 99%

“…In a previous work, we studied the antitumor activity of G. flavum in vitro and in vivo [10]. We performed a preliminary cytotoxic screening in vitro towards human breast cancer cells of different extracts of G. flavum .…”

Section: Introductionmentioning

confidence: 99%

Identification and Quantification of the Main Active Anticancer Alkaloids from the Root of Glaucium flavum

Bournine

Bensalem

Wauters

et al. 2013

IJMS

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Glaucium flavum is used in Algerian folk medicine to remove warts (benign tumors). Its local appellations are Cheqiq el-asfar and Qarn el-djedyane. We have recently reported the anti-tumoral activity of Glaucium flavum root alkaloid extract against human cancer cells, in vitro and in vivo. The principal identified alkaloid in the extract was protopine. This study aims to determine which component(s) of Glaucium flavum root extract might possess potent antitumor activity on human cancer cells. Quantitative estimation of Glaucium flavum alkaloids was realized by HPLC-DAD. Glaucium flavum effect on human normal and cancer cell viability was determined using WST-1 assay. Quantification of alkaloids in Glaucium flavum revealed that the dried root part contained 0.84% of protopine and 0.07% of bocconoline (w/w), while the dried aerial part contained only 0.08% of protopine, glaucine as the main alkaloid, and no bocconoline. In vitro evaluation of the growth inhibitory activity on breast cancer and normal cells demonstrated that purified protopine did not reproduce the full cytotoxic activity of the alkaloid root extract on cancer cell lines. On the other hand, bocconoline inhibited strongly the viability of cancer cells with an IC50 of 7.8 μM and only a low cytotoxic effect was observed against normal human cells. Our results showed for the first time that protopine is the major root alkaloid of Glaucium flavum. Finally, we are the first to demonstrate a specific anticancer effect of Glaucium flavum root extract against breast cancer cells, which can be attributed, at least in part, to bocconoline.

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“…For instance, in MCF7 cells the IC 50 values were in a range from 15.78 to 27.96 μg/mL (Talib and Mahasneh, 2010). A crude alkaloid extract from Glaucium flavum inhibited the growth of several human cancer cell lines in a dose dependent manner (IC 50 = 7.9–13.6 μg/mL; Bournine et al, 2013a). Furthermore, this type of extract significantly inhibited the growth of MDA-MB-231 cells in a dose-dependent manner (Bournine et al, 2013b).…”

Section: Scientific Evidence On Medicinal Plants Not Traditionally Usmentioning

confidence: 99%

North African Medicinal Plants Traditionally Used in Cancer Therapy

et al. 2017

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Background: Cancer is a major cause of mortality worldwide with increasing numbers by the years. In North Africa, the number of cancer patients is alarming. Also shocking is that a huge number of cancer patients only have access to traditional medicines due to several factors, e.g., economic difficulties. In fact, medicinal plants are widely used for the treatment of several pathologies, including cancer. Truthfully, herbalists and botanists in North African countries prescribe several plants for cancer treatment. Despite the popularity and the potential of medicinal plants for the treatment of cancer, scientific evidence on their anticancer effects are still scarce for most of the described plants.Objective: Bearing in mind the lack of comprehensive and systematic studies, the aim of this review is to give an overview of studies, namely ethnobotanical surveys and experimental evidence of anticancer effects regarding medicinal plants used in North Africa for cancer therapy.Method: The research was conducted on several popular search engines including PubMed, Science Direct, Scopus and Web of Science. The research focused primarily on English written papers published between the years 2000 and 2016.Results: This review on plants traditionally used by herbalists in North Africa highlights that Morocco and Algeria are the countries with most surveys on the use of medicinal plants in folk medicine. Among the plethora of plants used, Nigella sativa and Trigonella foenum-graecum are the most referred ones by herbalists for the treatment of cancer. Moreover, a plethora of scientific evidence qualifies them as candidates for further drug development. Furthermore, we report on the underlying cellular and molecular mechanisms.Conclusion: Overall, this review highlights the therapeutic potential of some medicinal plants as anticancer agents. The North African flora offers a rich source of medicinal plants for a wide array of diseases, including cancer. The elucidation of their modes of action represents an indispensable condition for the rational development of new drugs for cancer treatment. Furthermore, testing the anticancer activity in vivo and in clinical trials are warranted to explore the full therapeutic potential of North African plants for cancer therapy.

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Revealing the anti-tumoral effect of Algerian Glaucium flavum roots against human cancer cells

Cited by 19 publications

References 29 publications

Halofuginone induces the apoptosis of breast cancer cells and inhibits migration via downregulation of matrix metalloproteinase-9

Halofuginone induces the apoptosis of breast cancer cells and inhibits migration via downregulation of matrix metalloproteinase-9

Identification and Quantification of the Main Active Anticancer Alkaloids from the Root of Glaucium flavum

North African Medicinal Plants Traditionally Used in Cancer Therapy

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