Revealing new insights into different phosphorus-starving responses between two maize (Zea mays) inbred lines by transcriptomic and proteomic studies

Jiang, Huimin; Zhang, Jianfeng; Han, Zhuo; Yang, Juncheng; Ge, Cailin; Wu, Qingyu

doi:10.1038/srep44294

Cited by 27 publications

(28 citation statements)

References 31 publications

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“…Due to the promising plant growth-promoting effects of PGPM combinations in the previous experiment (Figure 3A), a range of consortium products were tested as microbial inoculants: Combifector-A (see Section 4.2) Combifector-B ( Trichoderma harzianum OMG16 + Bacillus amyloliquefaciens FZB42 + Zn/Mn), Bacillus amyloliquefaciens FZB42 + humic acids, and Bacillus amyloliquefaciens FZB42 + seaweed extract. Since many studies have demonstrated the importance of early root development as a critical trait determining yield formation of maize particularly with respect to P acquisition [33,34,35], special emphasis was placed on the selection of PSMs with additional root growth-promoting potential (Figure 3B).…”

Section: Discussionmentioning

confidence: 99%

The Form of N Supply Determines Plant Growth Promotion by P-Solubilizing Microorganisms in Maize

et al. 2019

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Phosphate-(P)-solubilizing microorganisms (PSM) are important drivers of P cycling in natural and agro-ecosystems. Their use as plant inoculants to improve P acquisition of crops has been investigated for decades. However, limited reproducibility of the expected effects, particularly under field conditions, remains a major challenge. This study demonstrates that the form of nitrogen fertilization has a significant impact on the performance of various fungal and bacterial PSM inoculants in maize grown on neutral to alkaline soils with limited P availability. Under these conditions, a high soil pH-buffering capacity frequently limits the efficiency of nutrient mobilization, mediated by plant roots and microorganisms via rhizosphere acidification. In a soil pH range between 7.0 and 8.0, nitrate fertilization promoting rhizosphere alkalinisation further aggravates this problem. Accordingly, in greenhouse experiments, six strains of Pseudomonas, Bacillus, Paenibacillus, Streptomyces, and Penicillium with proven P-solubilizing potential, completely failed to promote P acquisition in maize grown on a calcareous Loess sub-soil pH 7.6 with nitrate fertilization and rock phosphate (Rock-P) as a sparingly soluble P source. However, after replacement of nitrate fertilization by ammonium, stabilized with the nitrification inhibitor 3,4-dimethylpyrazole-phosphate (DMPP), five out of seven investigated PSM inoculants (comprising 12 fungal and bacterial PSM strains) exerted beneficial effects on plant growth and reached up to 88% of the shoot biomass production of a control supplied with soluble triple-superphosphate (TSP). Stabilized ammonium combined with PSM-inoculants improved P acquisition (Trichoderma harzianum T22, Pseudomonas sp. DMSZ 13134), while other strains particularly stimulated root growth (T. harzianum OMG16, Bacillus amyloliquefaciens FZB42), which promoted the acquisition also of other mineral nutrients, such as N, K, and Mn. A similar effect was recorded under field conditions on an alkaline clay-loam soil pH 8.6. The combination of stabilized ammonium with a range of consortium products based on T. harzianum OMG16, B. amyloliquefaciens, micronutrients, and humic acids completely compensated the effect of a TSP fertilization on field establishment, nutrient acquisition, and yield formation in maize, while non-stabilized urea-di-ammonium phosphate fertilization was largely ineffective. These findings suggest that the efficiency of PSM-plant interactions can be influenced by the form of N fertilization, offering promising perspectives for synergistic effects with stabilized ammonium fertilizers.

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Section: Discussionmentioning

confidence: 99%

The Form of N Supply Determines Plant Growth Promotion by P-Solubilizing Microorganisms in Maize

et al. 2019

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“…Above- and belowground dry matter production were statistically not different between cultivars without P ( Figure 5B ), and both cultivars had an intermediate PAE in the genotype screen, which might be the reason for similar behavior in root phosphatase activity. For maize, it was shown that low-P tolerant genotypes showed higher (Gaume et al, 2001; Jiang et al, 2017) or earlier (Du et al, 2016) root acid phosphatase activity than sensitive ones.…”

Section: Discussionmentioning

confidence: 99%

Genotype-Specific Differences in Phosphorus Efficiency of Potato (Solanum tuberosum L.)

et al. 2019

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Potato is considered to have a low phosphorus (P) efficiency compared to other crops. Therefore, P fertilization requirements are high. New cultivars with improved P efficiency may contribute to save limited mineral P sources and to reduce eutrophication of surface water bodies. The present study aims to characterize the P efficiency of different potato genotypes and to identify mechanisms that improve P efficiency in cultivated potato. A diversity set of 32 potato accessions was used to assess their P efficiency. From this set, five cultivars were selected and two pot experiments with different P-fertilization strategies including a non-fertilized control were conducted to estimate effects of P deficiency on general agronomic and P related traits, root development, phosphatase activity and micro RNA 399 (miR399) expression. Significant differences between the 32 genotypes were found for P utilization efficiency (PUtE). P acquisition efficiency (PAE) as P content in low P in relation to P content in high P was positively correlated to relative biomass production while PUtE was not. Selected genotypes displayed a strong relation between total root length and P content. Root phosphatase activity and miR399 expression increased under P deficiency. However, tuber yields of four cultivars, grown on a soil with suboptimal content of plant available P, were not significantly affected in comparison to yields of well-fertilized plots. We conclude from the present study that PUtE and PAE are important traits when selecting for plants requiring less fertilizer inputs but PAE might be more important for cropping on deficient soils. A large root system might be the most important trait for P acquisition on such soils and therefore in breeding for P efficient crops. Lowering P fertilizer inputs might not necessarily reduce tuber yields.

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“…Several time-course transcriptome (Woo et al, 2012 ; Secco et al, 2013 ; Ren et al, 2018 ), proteome (Iglesias et al, 2013 ; Jiang et al, 2017 ), and metabolome (Alexova et al, 2017 ) studies on Pi depletion and resupply are available. However, PTM studies that focus on temporal development of the Pi-stress response are limited to phosphorylation (Gregory et al, 2009 ; Yang J. et al, 2019 ), ubiquitination (Iglesias et al, 2013 ; Ye et al, 2018 ; Pan et al, 2019 ), and sumoylation (Kant et al, 2011 ; Feng et al, 2017 ; Datta et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Dynamic Responses of Barley Root Succinyl-Proteome to Short-Term Phosphate Starvation and Recovery

Wang

et al. 2021

Front. Plant Sci.

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Barley (Hordeum vulgare L.)—a major cereal crop—has low Pi demand, which is a distinct advantage for studying the tolerance mechanisms of phosphorus deficiency. We surveyed dynamic protein succinylation events in barley roots in response to and recovery from Pi starvation by firstly evaluating the impact of Pi starvation in a Pi-tolerant (GN121) and Pi-sensitive (GN42) barley genotype exposed to long-term low Pi (40 d) followed by a high-Pi recovery for 10 d. An integrated proteomics approach involving label-free, immune-affinity enrichment, and high-resolution LC-MS/MS spectrometric analysis was then used to quantify succinylome and proteome in GN121 roots under short-term Pi starvation (6, 48 h) and Pi recovery (6, 48 h). We identified 2,840 succinylation sites (Ksuc) across 884 proteins; of which, 11 representative Ksuc motifs had the preferred amino acid residue (lysine). Furthermore, there were 81 differentially abundant succinylated proteins (DFASPs) from 119 succinylated sites, 83 DFASPs from 110 succinylated sites, 93 DFASPs from 139 succinylated sites, and 91 DFASPs from 123 succinylated sites during Pi starvation for 6 and 48 h and during Pi recovery for 6 and 48 h, respectively. Pi starvation enriched ribosome pathways, glycolysis, and RNA degradation. Pi recovery enriched the TCA cycle, glycolysis, and oxidative phosphorylation. Importantly, many of the DFASPs identified during Pi starvation were significantly overexpressed during Pi recovery. These results suggest that barley roots can regulate specific Ksuc site changes in response to Pi stress as well as specific metabolic processes. Resolving the metabolic pathways of succinylated protein regulation characteristics will improve phosphate acquisition and utilization efficiency in crops.

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Revealing new insights into different phosphorus-starving responses between two maize (Zea mays) inbred lines by transcriptomic and proteomic studies

Cited by 27 publications

References 31 publications

The Form of N Supply Determines Plant Growth Promotion by P-Solubilizing Microorganisms in Maize

The Form of N Supply Determines Plant Growth Promotion by P-Solubilizing Microorganisms in Maize

Genotype-Specific Differences in Phosphorus Efficiency of Potato (Solanum tuberosum L.)

Dynamic Responses of Barley Root Succinyl-Proteome to Short-Term Phosphate Starvation and Recovery

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