Revealing antimicrobial resistance profile of the novel probiotic candidate Faecalibacterium prausnitzii DSM 17677

Machado, Daniela; Barbosa, Joana; Domingos, Melany; Almeida, Diana; Andrade, José Carlos; Freitas, Ana Cristina; Gomes, Ana

doi:10.1016/j.ijfoodmicro.2021.109501

Cited by 12 publications

(8 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Further, the L. plantarum (182) and Lactobacillus rhamnosus GG probiotic strains with mutations that altered the Dalanine display on lipoteichoic acid or lipoteichoic acid-deficient L. acidophilus probiotic strain (183) showed anti-inflammatory effects and prevented or reduced colitis symptoms in mice. In the same manner, Bifidobacterium breve has been reported to reduce pro-inflammatory cytokines (184) and LPS-induced epithelial cell shedding (185) while F. prausnitzii and sporeforming Clostridium clusters IV and XIV with strong antiinflammatory effects have been proposed as probiotic supplement to induce tolerance and lessening inflammatory symptoms (46,186,187). Probiotics have shown promising results in clinical trials related with several inflammatory conditions (Table 1).…”

Section: Potential Role Of Microbiome-targeted Interventions In Intes...mentioning

confidence: 99%

Dietary Component-Induced Inflammation and Its Amelioration by Prebiotics, Probiotics, and Synbiotics

2022

View full text Add to dashboard Cite

A balanced diet with many dietary components maintains immune homeostasis directly by interacting with innate and adaptive immune components or indirectly through gut microbiota and their metabolites. Dietary components may inhibit pro-inflammatory mediators and promote anti-inflammatory functions or vice versa. Western diets with imbalanced dietary components skew the immune balance toward pro-inflammation and induce intestinal inflammation, consequently leading to many intestinal and systemic inflammatory diseases like ulcerative colitis, Crohn’s disease, irritable bowel syndrome, cardiovascular problems, obesity, and diabetes. The dietary component-induced inflammation is usually chronic in nature and frequently caused or accompanied by alterations in gut microbiota. Therefore, microbiome-targeted therapies such as probiotics, prebiotics and synbiotics hold great potentials to amend immune dysregulation and gut dysbiosis, preventing and treating intestinal and systemic inflammatory diseases. Probiotics, prebiotics and synbioitcs are progressively being added to foods and beverages, with claims of health benefits. However, the underlining mechanisms of these interventions for preventing and treating dietary component-induced inflammation are still not very clear. In addition, possibly ineffective or negative consequences of some probiotics, prebiotics and synbiotics call for stringent testing and regulation. Here, we will first briefly review inflammation, in terms of its types and the relationship between different dietary components and immune responses. Then, we focus on current knowledge about the direct and indirect effects of probiotics, prebiotics and synbiotics on intestinal and systemic inflammation. Understanding how probiotics, prebiotics and synbiotics modulate the immune system and gut microbiota will improve our strategies for preventing and treating dietary component-induced intestinal inflammation and inflammatory diseases.

show abstract

Section: Potential Role Of Microbiome-targeted Interventions In Intes...mentioning

confidence: 99%

Dietary Component-Induced Inflammation and Its Amelioration by Prebiotics, Probiotics, and Synbiotics

2022

View full text Add to dashboard Cite

show abstract

“…This discrepancy can be partially explained by the different methods used for phenotypic testing (broth dilution in our work vs. E-test method in Cozzolino et al 22 ) or by the growth medium used (sIST in our work vs BHI Cozzolino et al 22 ). Disagreements caused by methodologies used for classifying microbial species into resistant or susceptible phenotype are present in the literature 19 , 44 . However, it is important to highlight that broth dilution method is widely accepted for phenotypic tests based on MIC determination.…”

Section: Resultsmentioning

confidence: 99%

“…Nevertheless, while genome mining for ARGs is routinely performed, standardized methods for the MIC evaluation have not yet been defined for this microorganisms. Furthermore, there is a lack of specific microbiological cut-off values to be used to distinguish strains with intrinsic or acquired resistance from susceptible strains 18 , 19 , mainly due to the limited number of available cultivable strains. Recently, EFSA Panel on Nutrition, Novel food, and Food Allergens (NDA) expressed a positive opinion on the safety of pasteurized A. muciniphila , opening the door for its use in food supplements and in food for special medical purposes 20 .…”

Section: Introductionmentioning

confidence: 99%

Characterization of antibiotic-resistance traits in Akkermansia muciniphila strains of human origin

Filardi

Gargari

Mora

et al. 2022

Sci Rep

View full text Add to dashboard Cite

Akkermansia muciniphila, a commensal bacterium commonly found in healthy gut microbiota, is widely considered a next-generation beneficial bacterium candidate to improve metabolic and inflammatory disorders. Recently the EFSA’s Panel on Nutrition, Novel food, and Food Allergens has declared that pasteurized A. muciniphila DSM 22959T (also MucT, ATCC BAA-835) can be considered safe as a novel food, opening the door to its commercialization as a food supplement. Despite its recognized health benefits, there is still little information regarding the antimicrobial susceptibility of this species and reference cut-off values to distinguish strains with intrinsic or acquired resistance from susceptible strains. In this study, we combined a genomic approach with the evaluation of the antibiotic susceptibility in five human A. muciniphila isolates. Genomic mining for antimicrobial resistance genes and MICs determinations revealed that only one strain harboring tetW gene showed resistance to tetracycline, whereas all A. muciniphila strains showed low sensitivity to ciprofloxacin and aminoglycosides with no genotypic correlation. Although all strains harbor the gene adeF, encoding for a subunit of the resistance-nodulation-cell division efflux pump system, potentially involved in ciprofloxacin resistance, the susceptibility towards ciprofloxacin determined in presence of efflux pump inhibitors was not affected. Overall, our outcomes revealed the importance to extend the antibiotic susceptibility test to a larger number of new isolates of A. muciniphila to better assess the safety aspects of this species.

show abstract

“…aureus (2.9%). Recently, Machado [ 41 ] detected 24 genes related to antimicrobials including aminoglycosides in F . prausnitzii .…”

Section: Discussionmentioning

confidence: 99%

Importance of Microbiome of Fecal Samples Obtained from Adolescents with Different Weight Conditions on Resistance Gene Transfer

et al. 2022

View full text Add to dashboard Cite

Antimicrobial resistance (AMR) is a relevant public health problem worldwide, and microbiome bacteria may contribute to the horizontal gene transfer associated with antimicrobial resistance. The microbiome of fecal samples from Mexican adolescents were analyzed and correlated with eating habits, and the presence of AMR genes on bacteria in the microbiome was evaluated. Fecal samples from adolescents were collected and processed to extract genomic DNA. An Illumina HiSeq 1500 system was used to determine resistance genes and the microbiome of adolescents through the amplification of gene resistance and the V3–V4 regions of RNA, respectively. Analysis of the microbiome from fecal samples taken from 18 obese, overweight, and normal-weight adolescents revealed that the Firmicutes was the most frequent phylum, followed by Bacteroidetes, Actinobacteria, Proteobacteria and Verrucomicrobia. The following species were detected as the most frequent in the samples: F. prausnitzii, P. cori, B. adolescentis, E. coli and A. muciniphila. The presence of Bacteroides, Prevotella and Ruminococcus was used to establish the enterotype; enterotype 1 was more common in women and enterotype 2 was more common in men. Twenty-nine AMR genes were found for β-lactamases, fluoroquinolones, aminoglycosides, macrolide, lincosamides, streptogramin (MLS), tetracyclines and sulfonamides. The presence of microorganisms in fecal samples that harbor AMR genes that work against antimicrobials frequently used for the treatment of microbial infections such as b-lactams, macrolides, aminoglycosides, MLS, and tetracyclines is of great concern, as these organisms may be an important reservoir for horizontal AMR gene transfer.

show abstract

Revealing antimicrobial resistance profile of the novel probiotic candidate Faecalibacterium prausnitzii DSM 17677

Cited by 12 publications

References 62 publications

Dietary Component-Induced Inflammation and Its Amelioration by Prebiotics, Probiotics, and Synbiotics

Dietary Component-Induced Inflammation and Its Amelioration by Prebiotics, Probiotics, and Synbiotics

Characterization of antibiotic-resistance traits in Akkermansia muciniphila strains of human origin

Importance of Microbiome of Fecal Samples Obtained from Adolescents with Different Weight Conditions on Resistance Gene Transfer

Contact Info

Product

Resources

About