Revascularization and periapical repair after endodontic treatment using apical negative pressure irrigation versus conventional irrigation plus triantibiotic intracanal dressing in dogs' teeth with apical periodontitis

Tissue characterization following revascularization of immature dog teeth using different disinfection pastes

“…It is likely that the more severe inflammatory process observed in the triple antibiotic paste group is due to the irritation caused by the antibiotic agents present in the triple paste. Similar results were reported by Silva et al 35 and Pereira et al 37 The latter authors, 37 characterizing rat subcutaneous tissue response after the use of triple antibiotic paste, observed a severe, persistent inflammatory process and no repair, confirming the paste's cytotoxicity. Also, it is possible that the paste concentration used in this study (20 mg/mL) has caused the less favorable results obtained in the triple antibiotic group with regard to severity of the inflammatory process and vital tissue formation in the root canal space.…”

“…18,19,28 Phumpatrakom and Srisuwan 36 have shown that, even at low concentrations, the triple paste affects cell proliferation in the pulp and apical papilla and the formation of mineralized matrices. Also, as suggested by Silva et al, 35 intracanal use of antibiotic medications is apparently not essential to MT formation. According to those authors, the use of triple antibiotic paste was associated with poorer results in terms of MT formation, apical development, and repair of periapical tissues when compared with a group not using the paste.…”

“…This finding was probably a result of the longer follow-up time in our study (7 months) when compared with previous ones with similar methodologies (usually the study period is of approximately 3 months). 1,10,33,35 According to Yamauchi et al, 32 in many cases, cementum-like MT formed on the dentin surface is thicker than the natural layer of radicular cementum; it remains to be known whether deposition of this type of tissue would continue until obstructing the canal lumen with a longer follow-up time. Possibly, in the long term, the direct contact between the two types of MT could cause replacement resorption inside the canal, in which cementum-like MT would be resorbed and replaced with bone-like MT as part of a bone remodeling process, similar to the replacement resorption process observed after traumatic injuries ( Figure 5).…”

The Microbial Challenge to Pulp Regeneration

“…The histological reports from these studies reported that the mineralized tissue observed histologically at 90 days post-operatively consisted of bone or cementum, and the soft tissues were periodontal ligament or inflamed connective tissues. No new pulp tissues were demonstrated (Thibodeau et al, 2007;da Silva et al, 2010;Wang et al, 2010). The revascularization techniques described in these studies do not use the classic tissueengineering triad, which consists of stem cells, scaffolds, and growth factors.…”

Avaliação in vivo da redução microbiana após preparo do canal radicular com auxílio do sistema EndoVac

“…Um novo estudo in vivo com dentes imaturos de cães foi realizado por Silva et al (2010). Os autores dividiram oitenta canais em quatro grupos: irrigação com NaOCl 2,5% e EndoVac (Grupo 1), irrigação convencional e NaOCl 2,5% + medicação com pasta triantibiótica (metronidazol, ciprofloxacina e minoxiclina) por 14 dias (Grupo 2), dentes hígidos sem tratamento (Grupo 3) e dentes com periodontite apical induzida sem tratamento (Grupo 4).…”