Revascularization and Function of Pancreatic Islet Isografts in Diabetic Rats Following Transplantation.

Furuya, Hidekazu; Kimura, Toshihisa; Morikawa, Mitsuhiro; Murakami, Makoto; Katayama, Kanji; Yamaguchi, Akio

doi:10.1097/00007890-200407271-01676

Cited by 12 publications

(14 citation statements)

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“…Therefore, rapid revascularization is crucial for islet engraftment, survival, and function post-transplantation (Brissova et al, 2004). Successful islet grafts have been observed to regenerate the microvasculature within 10 to 14 days of transplantation (Menger et al, 1994;Vajkoczy et al, 1995;Merchant et al, 1997;Beger et al, 1998;Furuya et al, 2003). However, the proportion of islets that restore their original vasculature upon transplantation is limited and variable.…”

Section: B Inadequate Revascularization Of Transplanted Isletsmentioning

confidence: 99%

Biological and Biomaterial Approaches for Improved Islet Transplantation

2006

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Section: B Inadequate Revascularization Of Transplanted Isletsmentioning

confidence: 99%

Biological and Biomaterial Approaches for Improved Islet Transplantation

2006

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“…This unique capillary network is crucial for the supply of oxygen and nutrients to the islet cells and for the dispersal of islet hormones to the systemic circulation [8]. The islet capillary system is, however, disrupted by isolation, and during in vitro culture islet endothelial cells de-differentiate or die [9][10][11]. The revascularisation process of islets is generally thought to occur over a period of 7-14 days post-transplantation [12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Better vascular engraftment and function in pancreatic islets transplanted without prior culture

Olsson

Carlsson

2005

Diabetologia

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Aims/hypothesis: Recent studies suggest that donor endothelial cells may contribute to islet graft revascularisation. Since islet endothelial cells disappear during culture, we hypothesised that transplantation of islets without prior culture is beneficial for their engraftment. Methods: Cultured (4-7 days) or freshly isolated islets (<4 h after donor pancreas extirpation) were syngeneically transplanted into Wistar-Furth rats and C57Bl/6 mice beneath the renal capsule. Islet graft revascularisation was evaluated by measuring vascular density, blood flow and tissue oxygen tension. Islet graft function was investigated by a minimal islet mass model in inbred mice (C57Bl/6). Results: Four days after implantation, the partial pressure of oxygen (pO 2 ) in the transplanted cultured islets was less than 10 mmHg (1.33 kPa), but tended to be higher in grafts composed of freshly isolated islets. The pO 2 in the grafts of freshly isolated islets had more than doubled 4 weeks later, whereas the pO 2 in the grafts of cultured islets remained at values similar to those recorded 4 days after transplantation. Transplanted freshly isolated islets also had a higher vascular density than transplanted cultured islets (∼40 vs ∼25% of that in endogenous islets) when investigated 1 month post-implantation. When applying a minimal islet mass model in inbred mice, 200 freshly isolated islets cured alloxan-diabetic mice in all cases, whereas only 33% of the group receiving similar numbers of cultured islets were cured. Conclusions/interpretation: Transplantation of pancreatic islets without prior culture is beneficial for their vascular engraftment and function.

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“…Formation of new vessels may be too slow to allow survival of an optimal number of cells. Furuya et al [15] demonstrated that the native endothelial cell in the graft islets reduced gradually, and disappeared 5 d after the transplantation. New blood vessels develop over a period of about 14 d, or even 4 wk which is not fast enough to prevent the marked loss of islet cells occurring during the first few days after transplantation [16] .…”

Section: Discussionmentioning

confidence: 99%