Reutealis trisperma press cake induced production of xylanase by Trichoderma reesei: Effect of C/N ratio and initial pH

Riadi, Lieke; Agustin, Yuana Elly; Kusuma, Leony Dita; Sutrisno, Paulina Filiana; Utami, Titie Prapti

doi:10.1063/1.5094992

Cited by 3 publications

(1 citation statement)

References 15 publications

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“…The growth kinetic parameters (μ) obtained under the different fermentation conditions shown in Table were used for a statistical analysis to find out the effect of the aeration rate and the carbohydrate/protein ratio of the substrate on the growth rate, as presented in Table S1. The lowest growth rate was obtained in the case of the SSF with a SC:WB ratio of 3:1 and aeration of 1.9 VKgM because of the effects of the carbon and nitrogen ratios in the substrate, carbon content, and moisture evaporation causing a loss of water for growth . It was observed that the growth rate of T.…”

Section: Resultsmentioning

confidence: 96%

High Production of Cellulase and Xylanase in Solid-State Fermentation by Trichoderma reesei Using Spent Copra and Wheat Bran in Rotary Bioreactor

Chysirichote

Phaiboonsilpa

Laosiripojana

2023

Ind. Eng. Chem. Res.

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The enzyme production for lignocellulose saccharification by solid-state fermentation (SSF) of a food manufacturing byproduct was successfully carried out in a 30 L rotary bioreactor. Defatted spent copra (SC) supplemented with wheat bran (WB) was used as a substrate for the SSF of Trichoderma reesei and aerated at various rates. Regression analysis showed that the carbohydrate/protein (C/P) ratio of the substrate and the supplied aeration rate were the important factors for producing the enzyme cocktail, including cellulases (FPase, CMCase, and cellobiase) and xylanase. The substrate containing SC:WB of 3:2 (or the C/P ratio of 5.4) and the aeration of 1.0 L kg −1 substrate min −1 were found to enhance the production of the enzymes up to 5.68, 8.66, 29.2, and 34.44 U g −1 of dry substrate for FPase, CMCase, cellobiase, and xylanase activities, respectively. This discovery provided a promising environment for other substrates to produce multi-enzymes for lignocellulosic saccharification. Additionally, mathematical models were generated to predict the saccharifying degree of the produced enzyme for lignocellulose saccharification.

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Section: Resultsmentioning

confidence: 96%

High Production of Cellulase and Xylanase in Solid-State Fermentation by Trichoderma reesei Using Spent Copra and Wheat Bran in Rotary Bioreactor

Chysirichote

Phaiboonsilpa

Laosiripojana

2023

Ind. Eng. Chem. Res.

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Immobilization of xylanase on acid pretreatment bentonite as green biocatalyst

Riadi

Chrisnasari

Kristanto

et al. 2022

INTERNATIONAL CONFERENCE ON INFORMATICS, TECHNOLOGY, AND ENGINEERING 2021 (InCITE 2021): Leveraging Smart Engineering

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Dissolved oxygen on xylanase production by Trichoderma reesei using Reutalis trisperma press cake as an additional substrate

Riadi

Agustin

Kok

et al. 2023

APJMBB

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Dissolved oxygen (DO) level in media plays a big role in the succession of the fermentation process by a microorganism. In this project, Trichoderma reesei was cultivated in two types of media, media A and B, both in 250 mL shake flask and 1.5 L fermenter at varying DO level. Media A and B contained the same carbon source and mineral salts, the difference between both media was the presence of press cake in Media B as urea and ammonium sulfate replacement, whereas the nitrogen sources in media A consists of urea and ammonium sulfate. The batch fermentation process was conducted in a 7.8 C/N ratio with an initial pH of 6. The purified enzyme was shown to convert xylan to xylose. Media B that contained Reutealis trisperma press cake gives a higher xylanase activity than media A without press cake, for both shake flask and fermenter cultivations. A xylanase activity of 101.07±1.65 U/mL and 39.30±0.64 U/mL was produced in a shake flask under 140 rpm for 96 hours of fermentation with ratio of 0.6 media B/flask volume and 0.6 media A/flask volume, respectively. A xylanase activity of 205.90±0.84 U/mL and 88.87±1.45 U/mL was produced in 72 hours at 0.8 L/min of aeration rate in a 1.5 L fermenter with media A and media B, respectively. Thus, this study identified the Reutealis trisperma cake can be used as a beneficial additional substrate and nitrogen sources. The increase in DO level, which indicated by increasing aeration rate, can generate a higher xylanase activity for both media in shake flask and fermenter.

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Reutealis trisperma press cake induced production of xylanase by Trichoderma reesei: Effect of C/N ratio and initial pH

Abstract: Edible coating chitosan and lycopene isolated from watermelon as a solution to extend fruit and vegetables storage time from after harvest damage

Cited by 3 publications

References 15 publications

High Production of Cellulase and Xylanase in Solid-State Fermentation by Trichoderma reesei Using Spent Copra and Wheat Bran in Rotary Bioreactor

High Production of Cellulase and Xylanase in Solid-State Fermentation by Trichoderma reesei Using Spent Copra and Wheat Bran in Rotary Bioreactor

Immobilization of xylanase on acid pretreatment bentonite as green biocatalyst

Dissolved oxygen on xylanase production by Trichoderma reesei using Reutalis trisperma press cake as an additional substrate

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