Reuse of E-Plate Cell Sensor Arrays in the xCELLigence Real-Time Cell Analyzer

Stefanowicz–Hajduk, Justyna; Adamska, Anna; Bartoszewski, Rafał; Ochocka, J. Renata

doi:10.2144/000114450

Cited by 26 publications

(24 citation statements)

References 27 publications

(17 reference statements)

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“…RTCA is a non‐invasive, impedance‐based biosensor system that can measure cell viability, migration, growth, spreading and proliferation. Changes in cell morphology and behaviour are continuously monitored in real time using microelectronics located in the wells of the RTCA E‐plates (Stefanowicz‐Hajduk et al ., ). As cells adhere to the biocompatible gold microelectrodes that line the well bottoms in an E‐plate, they impede the flow of electric current between electrodes.…”

Section: Methodsmentioning

confidence: 97%

l‐Homocysteine‐induced cathepsin V mediates the vascular endothelial inflammation in hyperhomocysteinaemia

Leng

et al. 2017

British J Pharmacology

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Section: Methodsmentioning

confidence: 97%

l‐Homocysteine‐induced cathepsin V mediates the vascular endothelial inflammation in hyperhomocysteinaemia

Leng

et al. 2017

British J Pharmacology

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“…Cells (Ishikawa, AN3CA and HEC1A) were seeded in E-plate 96 (ACEA Biosciences) at 10,000 cells/well in the corresponding medium supplemented with 5% FCS and the plate was monitored every 1 min for 5 h, then every 15 min for a total of 72 h (24). E-plates were used a maximum of 3 times, with the wells trypsinized and washed with PBS between each use, as previously described (25). Data was calculated using RTCA software 1.2, supplied with the instrument (ACEA Biosciences, San Diego, CA, USA; https://www.aceabio.com/products/xcelligence-rtca/) and exported for statistical analysis.…”

Section: Methodsmentioning

confidence: 99%

Galectin‑7 is elevated in endometrioid (type I) endometrial cancer and promotes cell migration

et al. 2018

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Endometrial cancer (EC) is the most commonly diagnosed gynecological malignancy in Australian women. Notably, its incidence and mortality rate is increasing. Despite this, there are limited treatment options for EC. Galectin-7 regulates tumorigenesis in numerous epithelial cancer types, but the role of galectin-7 has not been investigated in EC. It was hypothesized that galectin-7 expression would be altered in EC and contribute to the development of EC. Galectin-7 levels in EC and benign endometrium were quantified by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and ELISA. The effect of recombinant galectin-7 (1 µg/ml) on cell adhesion, proliferation, apoptosis (xCELLigence and flow cytometry), migration (wound healing assay) and gene expression (RT-qPCR) was investigated using three human EC cell lines (Ishikawa, HEC1A and AN3CA). Galectin-7 gene and protein expression was significantly elevated in Grade 3 EC, compared with benign tissues. Galectin-7 was almost undetectable in Ishikawa and AN3CA cells, but highly expressed by HEC1A cells. Recombinant galectin-7 had no significant effect on cell proliferation or apoptosis in any cell line, but significantly reduced cell adhesion in Ishikawa (at 4 and 6 h) and AN3CA (at 2, 3, 4 and 6 h). Galectin-7 significantly promoted Ishikawa migration and significantly elevated collagen type IV α 1 chain and intercellular adhesion molecule 1 (ICAM1) gene expression during wound healing. The present study demonstrated that galectin-7 production increased in EC with increasing cancer grade; therefore, galectin-7 may promote the metastasis of EC by reducing cell-cell adhesion and enhancing cell migration.

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“…The x CELLigence® RTCA DP system (Roche Applied Science, Penzberg, Germany) was used to automatically and continuously monitor live cell proliferation, growth, viability, migration, and spreading with characters of non-invasive, close to physiological status, a higher degree of accuracy, and lab free [29,30]. The cells transfected with siRNA-Control or si-BRWD3 were seeded on an E-plate- 16 Chromatin immunoprecipitation combining with Sequencing (ChIP-seq) assay…”

Section: Cell Proliferation and Invasion Detected With The Xcelligencmentioning

confidence: 99%

Integrative Networks by BRWD3 Regulates Cytoskeleton Organization and Cell Motility

Li¹,

Wang²,

Cui³

et al. 2020

Preprint

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BackgroundEukaryotic cytoskeleton forms and keeps cell shape, transports intracellular particles and organelles, determines cell motility and other important cellular events. A large number of regulators of cytoskeleton organization have been identified, but the detailed regulatory mechanism still remains obscure. Previous reports suggest that BRWD3 may be a regulator of cytoskeleton organization in Drosophila melanogaster, and influences cell shape. Therefore, we investigated the molecular network of BRWD3 regulating cytoskeleton organization.ResultsIn this study, we observed the alteration of cell shape, cell motility, and proliferation when BRWD3 was knocked down in MCF-7 and MDA-MB-231 cell lines. The cells were rounded, cell motility decreased when BRWD3 was knocked down. Using chromatin immunoprecipitation combining with sequencing, we found that BRWD3 influenced the cytoskeleton organization, cell shape, and cell motility through regulating expression of the cytoskeleton associative genes including ARF1, ABI2, ARPC3, ARPC1A, RHOC, MEF2C, and VIM.ConclusionsA molecular network by BRWD3 is sketched to elucidate that BRWD3 may not only regulate actin filament but also regulate microtubule and intermediate filament-based cytoskeleton organization. These efforts provide an overview of a BRWD3 network regulating cytoskeleton organization, cell shape and motility, and allow a better understanding of cytoskeleton (re)organization and pathogenesis of mental retardation X-linked 93 and relative carcinomas.

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Reuse of E-Plate Cell Sensor Arrays in the xCELLigence Real-Time Cell Analyzer

Cited by 26 publications

References 27 publications

l‐Homocysteine‐induced cathepsin V mediates the vascular endothelial inflammation in hyperhomocysteinaemia

l‐Homocysteine‐induced cathepsin V mediates the vascular endothelial inflammation in hyperhomocysteinaemia

Galectin‑7 is elevated in endometrioid (type I) endometrial cancer and promotes cell migration

Integrative Networks by BRWD3 Regulates Cytoskeleton Organization and Cell Motility

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