Reusable and highly sensitive SERS immunoassay utilizing gold nanostars and a cellulose hydrogel-based platform

Abstract

The development of robust and sensitive point-of-care testing platforms is necessary to improve patient care and outcomes. Surface-Enhanced Raman Scattering (SERS)-based immunosensors are especially suited for this purpose. Here, we...

Triangular gold nanoplates/two-dimensional nano mica platelets with a 3D lightning-rod effect as flexible nanohybrid substrates for SERS bacterial detection

“…8 In recent years, the application of SERS spectroscopy to biological detection has attracted increasing attention. [9][10][11] Spectral fingerprints are used to identify microorganisms by their types and then monitor their biochemical reactions that occur when they are attacked by foreign agents. 12 In addition, the SERS substrate can enhance the Raman signal by 10 6 to 10 10 times, and the signal can be detected without labeling, 13 which can reduce the time and cost of detection.…”

Hybrid Surface-Enhanced Raman Scattering Substrates for the Trace Detection of Ammonium Nitrate, Thiram, and Nile Blue

“… 5 − 7 In the recent years, Au nanostars have stimulated enormous attention in the fabrication of SERS substrates because of the induced high electric fields at their sharp tips, unlike their spherical, triangular, and nanorod counterparts. 8 − 10 In most of the previous studies, chemically synthesized nanoparticles (NPs) with different shapes were embedded on different substrates, such as glass, silicon, paper(s), fabrics, and so forth, for being used as SERS sensors for the detection of distinct analyte molecules. 6 , 11 − 14 Recently, a few novel strategies have been demonstrated for patterned SERS substrates coated with NPs of various shapes for the detection of diverse analytes.…”

“…Gold (Au) nanomaterials are the maximum studied nanotechnological tools in diverse fields including surface-enhanced Raman spectroscopy (SERS), catalysis, biosensing, and bioimaging due to easy synthesis methods, chemical stability, biocompatibility, and outstanding optical properties. − Particularly, the localized surface plasmonic resonance (SPR) property of Au nanomaterials can be tuned from the visible to near-infrared region depending on the morphology with respect to the size and shape. − In the recent years, Au nanostars have stimulated enormous attention in the fabrication of SERS substrates because of the induced high electric fields at their sharp tips, unlike their spherical, triangular, and nanorod counterparts. − In most of the previous studies, chemically synthesized nanoparticles (NPs) with different shapes were embedded on different substrates, such as glass, silicon, paper(s), fabrics, and so forth, for being used as SERS sensors for the detection of distinct analyte molecules. ,− Recently, a few novel strategies have been demonstrated for patterned SERS substrates coated with NPs of various shapes for the detection of diverse analytes. − The patterned substrates along with NPs with diverse shapes have demonstrated a significant improvement in the detection sensitivity due to the embedded NPs, along with the patterned areas acting as hotspot regions and increasing the SPR strength, resulting in strong electric fields at the confined regions. − Abundant fabrication procedures have been established for fabricating patterned substrates on countless materials, among which short/ultrashort laser ablation is a simple, fast, and efficient fabrication tool for the generation of distinct surface structures on various substrates. − Since the past 2 decades, substantial improvements have been made in achieving diverse nanostructures (NSs) on a wide range of materials such as metals, semiconductors, insulators, and polymers by varying the short/ultrashort laser ablation parameters (pulse duration, wavelength, fluence, number of pulses, and focusing conditions) and surrounding environments (air, gas, and liquid). ,,− The ultrashort laser-induced surface structures have proven to be promising candidates in diverse areas such as sensing, ,− …”

Hybridizing Silver Nanoparticles in Hydrogel for High-Performance Flexible SERS Chips

“…Although the developed hydrogel SERS chip has been proven to possess many advantages including high sensitivity, excellent repeatability and reproducibility, and long-term stability, the applicability should be further evaluated by testing some pollutants from real samples. , Thiram and TBZ are two kinds of fungicide commonly used to control fungal diseases in fruit development and postharvest storage. However, both of them are potentially carcinogenic and teratogenic substances to humans .…”