Returning to the Wild: Restoring Natural Levels of Immune Function to Laboratory Animals

Ren, Daniel; Beinart, Dylan; Pi, Cindy; Poulton, Susan; Holzknecht, Zoie E.; Swanson, Chelsea; Parker, William F.

doi:10.9734/jalsi/2017/36983

Cited by 1 publication

(2 citation statements)

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“…Previously, similar approaches had been applied for wild rats. [36][37][38] The standard commercial inbred mouse or rat lives in very clean animal facilities under a specified pathogen free (SPF) regime (typically screened for a list of 20-50 pathogens). Furthermore, animals are often used at young age, from 8 to 12 weeks onwards.…”

Section: Pathogen Experience and Wild Microbiotamentioning

confidence: 99%

“…Finally, in a most important broad development, work with wild mice and pet shop mice demonstrates the crucial importance of pathogen experience, that is the exposure to a wide array of pathogens over a protracted period in a physiological setting to more adequately mimic the human immune system 34 (review by Graham 35 ). Previously, similar approaches had been applied for wild rats 36–38 . The standard commercial inbred mouse or rat lives in very clean animal facilities under a specified pathogen free (SPF) regime (typically screened for a list of 20–50 pathogens).…”

Section: Innovative Animal Models—pathogen Experience and “Wild” Micr...mentioning

confidence: 99%

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Cutting edge technologies in chronic inflammation research

Laman

2022

Experimental Dermatology

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This concise review provides the broad background and selection from the literature for a Keynote lecture at EHSF 2022 on state of the art technologies in inflammation research, with an emphasis on disease of the skin and the nervous system. The value of ex vivo skin explant models is discussed, as well as the innovative use of animal models, wherein the crucial roles of antigen experience and “wild” microbiota are emphasized. Spectral flow cytometry allowing large surface marker panels to be explored is touched upon, as well as multiplex technology for cytokines and other analytes important for inflammation and tissue damage. Single‐cell sequencing and in situ transcriptomics (spatial profiling) now provide exciting granular information on functional cell subsets, interactions and plasticity. A selection of novel research and diagnostic tools for antibodies against linear peptides or gangliosides is presented. Finally, the review discusses a new anti‐inflammatory strategy against skin inflammation with a panel of protease inhibitors derived from the protein fraction of industrial starch potatoes.

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Section: Pathogen Experience and Wild Microbiotamentioning

confidence: 99%