Retrovirally introduced antisense integrin RNA inhibits neuroblast migration in vivo

Galileo, Deni S.; Majors, John; Horwitz, Alan F.; Sanes, Joshua R.

doi:10.1016/0896-6273(92)90070-t

Cited by 146 publications

(104 citation statements)

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“…The [31-related RGD-blocking peptides have been shown to perturb mesencephalic neural crest migration (Boucaut et al, 1984). The neurite outgrowth and neural crest migration can also be perturbed by anti-131 antibodies or by corresponding antisense ODNs (Hall et al, 1987;Bronner-Fraser, 1994;Galileo et al, 1992). Besides 13 integrins, the functional activities of ct subunits have been elucidated by such methods as well.…”

Section: Discussionmentioning

confidence: 99%

Cloning of mouse integrin alphaV cDNA and role of the alphaV-related matrix receptors in metanephric development.

Wada

Kumar

Liu

et al. 1996

The Journal of Cell Biology

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Abstract. Metanephrogenesis has been a long-standing model to study cell-matrix interactions. A number of adhesion molecules, including matrix receptors (i.e., integrins), are believed to be involved in such interactions. The integrins contain a and ~ subunits and are present in various tissues in different heterodimeric forms. In this study, one of the members of the integrin superfamily, av, was characterized, and its relevance in murine nephrogenesis was investigated. Mouse embryonic renal eDNA libraries were prepared and screened for av, and multiple clones were isolated and sequenced. The deduced amino acid sequence of the et v cDNA clones and hydropathic analysis revealed that it has a typical signal sequence and extracellular, transmembrane, and cytoplasmic domains, with multiple Ca 2÷ binding sites. No A(U)nA mRNA instability motifs were present. Conformational analysis revealed no rigid long-range-ordered structure in murine O~v. The O~v was expressed in the embryonic kidney at day 13 of the gestation, with a transcript size of ~7 kb. Its expression increased progressively during the later gestational stages and in the neonatal period. It was distributed in the epithelial elements of developing nephrons and was absent in the uninduced mesenchyme. In mature metanephroi, the expression was relatively high in the glomeruli and blood vessels, as compared to the tubules. Various heterodimeric associations of av, i.e., with [31, [33, [35, and [36, were observed in metanephric tissues. Inclusion of av-antisense-oligodeoxynucleotide or -antibody in metanephric culture induced dysmorphogenesis of the kidney with reduced population of the nephrons, disorganization of the ureteric bud branches, and reduction of mRNA and protein expressions of etv. The expressions of integrin [33, [35, and 136 were unaltered. These findings suggest that the integrin av is developmentally regulated, has a distinct spatio-temporal expression, and is relevant in the mammalian organogenesis.URING development, the interactions between cell adhesion molecules and extraceUular matrix (ECM) 1 glycoproteins are believed to be essential for the morphogenesis of various organs (Hay, 1991;Humphries et al., 1991;Reichardt and Tomaselli, 1991;Ruoslahti, 1991;Toole, 1991;Hynes, 1992;Juliano and Haskill, 1993;Kramer et al., 1993;Gladson and Cheresh, 1994;Hemler et al., 1994;Quaranta et al., 1994). The maturation of the mammalian metanephros constitutes many of the steps which are prototypic of the events relevant to the development of other organ systems (Ekblom, 1993;Clapp and Abrahamson, 1994). The renal organogenesis ensues after an initial reciprocal induction between the metanephric Address all correspondence to Dr. Y.S. Kanwar, Department of Pathology, Northwestern University Medical School, 303 East Chicago Avenue, Chicago, IL 60611. Tel.: (312) 503-0084. Fax: (312) 503-0627.1. Abbreviations used in this paper: ECM, extracellular matrix; ODN, oligodeoxynucleotide; RT, reverse transcriptase. mesenchyme and the ureteric bud, which leads to...

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Section: Discussionmentioning

confidence: 99%

Cloning of mouse integrin alphaV cDNA and role of the alphaV-related matrix receptors in metanephric development.

Wada

Kumar

Liu

et al. 1996

The Journal of Cell Biology

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“…Tectal cells were dissociated and cultured using methods similar to those described by Galileo et al (1992). Briefly, tecta were removed from E l 0 embryos, minced in sterile Ca2' -and Mg2+-free Tyrode solution, and incubated in 0.1% trypsin plus 0.05% EDTA for 15 min a t 37°C.…”

Section: Cell Culturesmentioning

confidence: 99%

Developmentally regulated and spatially restricted antigens of radial glial cells

Herman

Victor

Sanes

1993

Developmental Dynamics

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Radial glial cells, present in many parts of the embryonic vertebrate central nervous system (CNS), have been implicated in the guidance of neuroblasts from the ventricular zone to their laminar destinations. Moreover, radial glial cells may be progenitors of some CNS neurons and glia. To gain new insight into the structure and development of these cells, we have generated and characterized a panel of monoclonal antibodies that recognize radial glial cells of the chick optic tectum. Mice were immunized with homogenates of embryonic day (E) 10 tectum, and antibodies were analyzed by immunofluorescence and immunoblotting. We describe here three pairs of antibodies. 1) H5 and a previously generated antibody, R5 (Drager et al., J. Neurosci. 42025, 1984), stain the whole extent of the radial glial cell from E7 to E20. In cultures prepared from El0 tecta, both stain a filamentous meshwork in glial cells but not in neurons. On immunoblots, both recognize a protein of -52 kD that is closely related (or identical) to vimentin. 2) H28 and H29 stain radial glia between E7 and E14, but not later. Moreover, H28 and H29 staining is markedly more intense in the ventricular and intermediate zones than in the laminae of the tectal plate. Both of these antibodies recognize an intracellular epitope in cultured glial cells and a protein of -35 kD on immunoblots. 3) H2 and H27 recognize antigens concentrated in the most superficial processes and endfeet of radial glia in late (E16-E20) embryos. They stain distinct structures in cultured glia, suggesting that they recognize distinct antigens. H27 recognizes a protein of -29 kD on immunoblots. Thus antibodies H5 and R5 are good markers of radial glial cells at all stages, whereas the others define antigens that are developmentally regulated and localized to discrete domains. Together, these antibodies can be used to study temporal and spatial specializations of radial glia. o 1993 %ley-Liss, Inc.

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“…In the nervous system, they play important roles in basal lamina assembly and neuronal migration (Galileo et al, 1992;Anton et al, 1999;Graus-Porta et al, 2001;Sanada et al, 2004;Schmid et al, 2004), axonal growth and dendritic development (Tomaselli et al, 1993;DeFreitas et al, 1995;VarnumFinney et al, 1995;Weaver et al, 1995;Baum and Garriga, 1997;Hoang and Chiba, 1998;Pasterkamp et al, 2003;Moresco et al, 2005;Marrs et al, 2006), neuromuscular junction (NMJ) development and function (Chen and Grinnell, 1995;Martin and Sanes, 1997;Beumer et al, 1999;Rohrbough et al, 2000;Schwander et al, 2004), dendritic spine plasticity (Kramar et al, 2006;Shi and Ethell, 2006), and learning and memory (Grotewiel et al, 1998;Chan et al, 2003;Chan et al, 2006). At central synapses, pharmacological studies have indicated that RGD (Arg-GlyAsp)-binding integrins play an acute modulatory role in hippocampal long-term potentiation (LTP) (Bahr et al, 1997;Staubli et al, 1998;Kramar et al, 2003;Nagy et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Distinct Roles of the β1-Class Integrins at the Developing and the Mature Hippocampal Excitatory Synapse

Huang¹,

Shimazu²,

Woo³

et al. 2006

J. Neurosci.

141

150

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Integrins are a large family of cell adhesion receptors involved in a variety of cellular functions. To study their roles at central synapses, we used two cre recombinase lines to delete the Itgb1 ␤1 integrin gene in forebrain excitatory neurons at different developmental stages. Removal of the ␤1 integrins at an embryonic stage resulted in severe cortical lamination defects without affecting the cellular organization of pyramidal neurons in the CA3 and CA1 regions of the hippocampus. Whereas the hippocampal neurons underwent normal dendritic and synaptic differentiation, the adult synapses exhibited deficits in responses to high-frequency stimulation (HFS), as well as in long-term potentiation (LTP). Deletion of ␤1 integrin at a later postnatal stage also impaired LTP but not synaptic responses to HFS. Thus, the ␤1-class integrins appear to play distinct roles at different stages of synaptic development, critical for the proper maturation of readily releasable pool of vesicles during early development but essential for LTP throughout adult life.

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Retrovirally introduced antisense integrin RNA inhibits neuroblast migration in vivo

Cited by 146 publications

References 47 publications

Cloning of mouse integrin alphaV cDNA and role of the alphaV-related matrix receptors in metanephric development.

Cloning of mouse integrin alphaV cDNA and role of the alphaV-related matrix receptors in metanephric development.

Developmentally regulated and spatially restricted antigens of radial glial cells

Distinct Roles of the β1-Class Integrins at the Developing and the Mature Hippocampal Excitatory Synapse

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