Retroviral Vectors in Gene Therapy

Gopinath, Chitra; Nathar, Trupti Job; Nelson, Everette Jacob Remington

doi:10.1007/978-981-13-0481-1_1

Cited by 3 publications

(5 citation statements)

References 72 publications

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“…In spite of their advantages, viral vectors also have disadvantages. As lentiviruses and retroviruses enter the target cell genome, they can most likely cause a gene disorder 53,54 . Adeno‐associated virus vectors have lower risks than these two vectors; however, there is still insertional mutagenesis risk.…”

Section: Gene Therapy: Concept and Historymentioning

confidence: 99%

Gene therapy: A promising approach for breast cancer treatment

Dastjerd

Valibeik

Monfared

et al. 2021

Cell Biochemistry & Function

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Breast cancer (BC) is the most prevalent malignancy and the second leading cause of death among women worldwide that is caused by numerous genetic and environmental factors. Hence, effective treatment for this type of cancer requires new therapeutic approaches. The traditional methods for treating this cancer have side effects, therefore so much research have been performed in last decade to find new methods to alleviate these problems. The study of the molecular basis of breast cancer has led to the introduction of gene therapy as an effective therapeutic approach for this cancer. Gene therapy involves sending genetic material through a vector into target cells, which is followed by a correction, addition, or suppression of the gene. In this technique, it is necessary to target tumour cells without affecting normal cells. In addition, clinical trial studies have shown that this approach is less toxic than traditional therapies. This study will review various aspects of breast cancer, gene therapy strategies, limitations, challenges and recent studies in this area.

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Section: Gene Therapy: Concept and Historymentioning

confidence: 99%

Gene therapy: A promising approach for breast cancer treatment

Dastjerd

Valibeik

Monfared

et al. 2021

Cell Biochemistry & Function

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“…In the original implementation of DUMAS (DUal acquisition Magic Angle Spinning) experiment 29 , the 13 C and 15 N polarization from SIM-CP was used to acquire 2D 13 C-and 15 N-edited experiments such as DARR and NCA with 13 C detection at slow MAS rates (8)(9)(10)(11)(12). Analogously, the H-DUMAS experiment enables the acquisition of 2D 1 H-detected 13 C-and 15 N-edited experiments (Figure 2A).…”

Section: H-detected Dumas (H-dumas): Simultaneous Acquisition Of (H)ch and (H)nh Experimentsmentioning

confidence: 99%

“…Solid-state NMR spectroscopy is a powerful tool to study the structure and dynamics of biomacromolecules that are recalcitrant to crystallization such as fibrillar and integral membrane proteins [1][2][3][4][5][6][7][8][9][10][11] . In the past decades, heteronuclear detected experiments (direct detection) have represented the main approach to determine resonance assignments and obtain internuclear distances for structure calculations 10,[12][13][14][15][16] .…”

Section: Introductionmentioning

confidence: 99%

“…During the past decade, our group has been developing 13 C-detected Polarization Optimized experiments (POE) aiming to speed up the spectroscopy of membrane proteins reconstituted with lipid-to-protein ratios close to physiological conditions 9,[28][29][30][31][32][33][34][35][36] . Our overall goal has been to adapt ssNMR spectroscopy samples to closely resemble physiological conditions, rather than adapting the samples to the needs of spectroscopy.…”

Section: Introductionmentioning

confidence: 99%

“…The beauty of POE comes from its straightforward implementation on commercial spectrometers equipped with only one receiver and Low-E or E-free MAS probes [40][41] . As a result, POE are becoming more popular and have been successfully implemented for 1 H-detected experiments, including hybrid pulse sequences with a combination of CP and INEPT polarization transfer periods 28,[42][43][44][45][46] .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Proton-detected polarization optimized experiments (POE) using ultrafast magic angle spinning solid-state NMR: Multi-acquisition of membrane protein spectra

Gopinath

Veglia

2020

Journal of Magnetic Resonance

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Proton-detected solid-state NMR (ssNMR) spectroscopy has dramatically improved the sensitivity and resolution of fast magic angle spinning (MAS) methods. While relatively straightforward for fibers and crystalline samples, the routine application of these techniques to membrane protein samples is still challenging. This is due to the low sensitivity of these samples, which require high lipid:protein ratios to maintain the structural and functional integrity of membrane proteins. We previously introduced a family of novel polarization optimized experiments (POE) that enable to make the best of nuclear polarization to obtain multiple-acquisitions from a single pulse sequence and one receiver. Here, we present the 1 H-detected versions of POE using ultrafast MAS ssNMR. Specifically, we implemented proton detection into our three main POE strategies, H-DUMAS, H-MEIOSIS, and H-MAeSTOSO, achieving the acquisition of up to ten different experiments using a single pulse sequence. We tested these experiments on a model compound N-Acetyl-Val-Leu dipeptide and applied to a six transmembrane acetate transporter, SatP, reconstituted in lipid membranes. These new methods will speed up the spectroscopy of challenging biomacromolecules such as membrane proteins.

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Biomanufacturing Aspects of Gene Therapy

Warnock

2023

Cell Engineering

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Retroviral Vectors in Gene Therapy

Cited by 3 publications

References 72 publications

Gene therapy: A promising approach for breast cancer treatment

Gene therapy: A promising approach for breast cancer treatment

Proton-detected polarization optimized experiments (POE) using ultrafast magic angle spinning solid-state NMR: Multi-acquisition of membrane protein spectra

Biomanufacturing Aspects of Gene Therapy

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