Retroviral-mediated gene transfer of the peripheral myelin protein PMP22 in Schwann cells: modulation of cell growth.

Zoidl, Georg; Blass-Kampmann, Sabine; D’Urso, Donatella; Schmalenbach, Corinne; Müller, Hans

doi:10.1002/j.1460-2075.1995.tb07095.x

Cited by 176 publications

(136 citation statements)

References 44 publications

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“…However, it should be pointed out that PMP22/gas3, which has been associated with the induction of apoptosis in Schwann cells (Fabbretti et al, 1995), is down-regulated here. Interestingly, the underexpression of the PMP22/gas3 gene is known to increase the proportion of cells that enter the S+G2/M phases (Zoidl et al, 1995), a situation recently found to occur during the course of the cell death program studied here (Baudet et al, 1996b).…”

Section: Osteonectin/sparc and Dynein Heavy Chain/map 1c Genesmentioning

confidence: 63%

Differentially expressed genes in C6.9 glioma cells during vitamin D-induced cell death program

et al. 1998

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C6.9 rat glioma cells undergo a cell death program when exposed to 1,25-dihydroxyvitamin D3 (1,25-D3). As a global analytical approach, we have investigated gene expression in C6.9 engaged in this cell death program using differential screening of a rat brain cDNA library with probes derived from control and 1,25-D3-treated cells. Using this methodology we report the isolation of 61 differentially expressed cDNAs. Forty-seven cDNAs correspond to genes already characterized in rat cells or tissues. Seven cDNAs are homologous to yeast, mouse or human genes and seven are not related to known genes. Some of the characterized genes have been reported to be differentially expressed following induction of programmed cell death. These include PMP22/gas3, MGP and b-tubulin. For the first time, we also show a cell death program induced up-regulation of the c-myc associated primary response gene CRP, and of the proteasome RN3 subunit and TCTP/mortalin genes. Another interesting feature of this 1,25-D3 induced-cell death program is the down-regulated expression of transcripts for the microtubule motor dynein heavy chain/MAP 1C and of the calcium-binding S100b protein. Finally 15 upregulated cDNAs encode ribosomal proteins suggesting a possible involvement of the translational apparatus in this cell program. Alternatively, these ribosomal protein genes could be up-regulated in response to altered rates of cellular metabolism, as has been demonstrated for most of the other isolated genes which encode proteins involved in metabolic pathways. Thus, this study presents to our knowledge the first characterization of genes which are differentially expressed during a cell death program induced by 1,25-D3. Therefore, this data provides new information on the fundamental mechanisms which participate in the antineoplastic effects of 1,25-D3 and on the machinery of a cell death program in a glioma cell line.

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Section: Osteonectin/sparc and Dynein Heavy Chain/map 1c Genesmentioning

confidence: 63%

Differentially expressed genes in C6.9 glioma cells during vitamin D-induced cell death program

et al. 1998

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“…The analysis of RNA integrity and cDNA synthesis has been described previously (37)(38). Zinc finger motif-containing sequences were amplified by PCR using standard reaction conditions for Pfu DNA polymerase (Promega) including 25 pmol of primer CysX 2 (5Ј-TGCCCNGAGTGYGGNAAR-3Ј), H/C (5Ј-NGGCTTCTCNCCNG-TATG-3Ј), and 1 l of the first strand cDNA synthesis reaction.…”

Section: Methodsmentioning

confidence: 99%

“…Samples containing water or RNA were included as controls. Equal cDNA use was confirmed by amplification of 18 S rRNA by PCR (37). For the postnatal time course, primers were as follows: ZFP57-USP (5Ј-GCATTACCAAACAATGGCAGCTAG-3Ј), ZFP-57-DSP (5Ј-AGGCCTCTCTCCACGATGCTGAGCC-3Ј) for Zfp-57, and GAPDH-USP (5Ј-CCTTCATTGACCTCAACTACATGGT-3Ј), GAPDH-DSP (5Ј-TCATTGTCATACCAGGAAATGAGCT-3Ј) as an invariant control.…”

Section: Methodsmentioning

confidence: 99%

Identification and Characterization of ZFP-57, a Novel Zinc Finger Transcription Factor in the Mammalian Peripheral Nervous System

Alonso

Zoidl

Taveggia

et al. 2004

Journal of Biological Chemistry

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To isolate new zinc finger genes expressed at early stages of peripheral nerve development, we have used PCR to amplify conserved zinc finger sequences. RNA from rat embryonic day 12 and 13 sciatic nerves, a stage when nerves contain Schwann cell precursors, was used to identify several genes not previously described in Schwann cells. One of them, zinc finger protein (ZFP)-57, proved to be the homologue of a mouse gene found in F9 teratocarcinoma cells. Its mRNA expression profile within embryonic and adult normal and transected peripheral nerves, and its distribution in the rest of the nervous system is described. High levels of expression are seen in embryonic nerves and spinal cord. These drop rapidly during the first few weeks after birth, a pattern mirrored in other parts of the nervous system. ZFP-57 localizes to the nucleus of Schwann and other cells. The sequence contains an N-terminal Krü ppel-associated box (KRAB) domain and ZFP-57 constructs containing green fluorescent protein reveal that the protein colocalizes with heterochromatin protein 1␣ to centromeric heterochromatin in a characteristic speckled pattern in NIH3T3 cells. The KRAB domain is required for this localization, because constructs lacking it target the protein to the nucleus but not to the centromeric heterochromatin. When fused to a heterologous DNA binding domain, the KRAB domain of ZFP-57 represses transcription, and fulllength ZFP-57 represses Schwann cell transcription from myelin basic protein and P 0 promoters in co-transfection assays. Zfp-57 mRNA is up-regulated in Schwann cells in response to leukemia inhibitory factor and fibroblast growth factor 2.The major stages in the embryonic development of Schwann cells in rodent peripheral nerves have been defined at the cellular level. Nearly all cells isolated from nerves of embryo day (E) 1 14/E15 rats or E12/E13 mice are Schwann cell precursors (1-4). They are derived from the neural crest and give rise to the immature Schwann cells of late embryonic and early postnatal nerves. These cells are the source of the myelinating or non-myelinating cells of adult nerves. Schwann cell precursors can be distinguished from immature Schwann cells on the one hand and from neural crest cells on the other by a number of criteria, including differences in the regulation of survival and DNA synthesis and antigenic phenotype (5-8).A number of transcription factors are involved in the cellular transitions that take place in embryonic nerves and early postnatal nerves (9, 10). The HMG domain factor Sox-10 is expressed in most or all neural crest cells and is required for the formation of the glial lineage (11,12). It is also likely to be important in later stages of development, because it has the capacity to synergize with Krox-20 (Egr-2) (see below) to activate the connexin 32 promoter (13-15). Two transcription factors, the POU domain proteins Oct-6 (SCIP, Tst-1, Pou3f1) and Brn-2 are involved in the timing of myelination (16 -18), whereas Schwann cells in nerves of mice that are null for the zi...

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“…Plasmid and phagemid DNA were purified uscell cycle in cultured Schwann cells (Ciccarelli et al, ing Wizard miniprep or midiprep kits (Promega). Manual sequencing 1990; Zoidl et al, 1995). Recently, it has been demonwas performed using the dideoxynucleotide chain-termination strated that overexpression of PMP22 in NIH 3T3 cells method and the Sequenase Quick-denature plasmid sequencing kit results in apoptosis (Fabbretti et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

cDNA Cloning, Genomic Structure, and Chromosome Mapping of the Human Epithelial Membrane Protein CL-20 Gene (EMP1), a Member of the PMP22 Family

et al. 1997

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tissues. Early in this terminal pathway of differentia-CL-20 is a novel gene encoding a protein that is struc-tion, cells undergo irreversible growth arrest that is turally related to but distinct from the peripheral myelin accompanied by a down-regulation of several cell cycle protein PMP22. Like PMP22, CL-20 is likely to play im-control genes, including RB and cdc2 (Jetten et al., portant roles in the regulation of cell proliferation, dif-1992; Saunders and Jetten, 1994). This is followed by ferentiation, and cell death. In this study, we describe the induction of differentiation-specific genes such as the cloning and sequencing of a cDNA encoding the hu-expression of several keratins and proteins involved man homologue of CL-20 and characterize the genomic in the formation of the cross-linked envelope such as structure of this gene. The hCL-20 gene (HGMW-ap-transglutaminase type I and cornifins (Eckert and proved symbol EMP1) encodes a protein of 157 amino Rorke, 1989;Jetten et al., 1992;Marvin et al., 1992). et al., 1995), which in several human tissues by Northern analysis. Retinoic might be the rat homologue of CL-20. The rabbit CLacid, which inhibits squamous differentiation, represses 20 exhibits moderate (43%) homology to the human CL-20 expression in normal human bronchial epithelial peripheral myelin protein, PMP22 protein (also known cells. The genomic structure of the hCL-20 gene was ana-as gas3) (Manfioletti et al., 1990; Welcher et al., 1991; lyzed using a P1 vector containing this gene. The hCL- Suter et al., 1992). CL-20, EMP-1, 20 gene contains five exons about 0.2, 0.12, 0.1, 0.14, and and PMP22 exhibit a great structural similarity, sug-2.2 kb and four introns about 15, 1.9, 0

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Retroviral-mediated gene transfer of the peripheral myelin protein PMP22 in Schwann cells: modulation of cell growth.

Cited by 176 publications

References 44 publications

Differentially expressed genes in C6.9 glioma cells during vitamin D-induced cell death program

Differentially expressed genes in C6.9 glioma cells during vitamin D-induced cell death program

Identification and Characterization of ZFP-57, a Novel Zinc Finger Transcription Factor in the Mammalian Peripheral Nervous System

cDNA Cloning, Genomic Structure, and Chromosome Mapping of the Human Epithelial Membrane Protein CL-20 Gene (EMP1), a Member of the PMP22 Family

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