Retroviral Infection of Primary Hepatocytes from Normal Mice and Mice Transgenic for SV40 Large T Antigen

Weber-Benarous, A; Decaux, Jean‐François; Bennoun, Myriam; Allemand, Isabelle; Briand, Pascale; Kahn, Axel

doi:10.1006/excr.1993.1062

Cited by 17 publications

(5 citation statements)

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“…12 The cells were fixed in either 10% buffered formalin or in Bouin's liquid, and after centrifugation in an eppendorf tube the pellet was included in a paraffin block. The second positive control consisted of a routinely processed (fixed in Bouin's liquid and paraffin-embedded) kidney biopsy from a kidney transplant patient with an active infection with SV40 (confirmed by PCR in urine).…”

Section: Methodsmentioning

confidence: 99%

Immunohistochemical investigation of SV40 large T antigen in Hodgkin and non‐Hodgkin's lymphoma

Brousset

Araujo

Gascoyne

2004

Intl Journal of Cancer

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DNA sequences from simian virus 40 (SV40) have been detected in various human tumors, including non-Hodgkin's lymphomas (NHLs), by highly sensitive PCR techniques. However, there is a strong debate as to whether SV40 is present in lymphoma cells. Using immunohistochemistry and tissue microarrays, we investigated a series of French and Canadian cases of Hodgkin's lymphomas (HLs) and NHLs and tried to detect the SV40 large T antigen using routine paraffin sections with standard and highly sensitive catalyzed system amplification methods. None of the cases of HLs (n ‫؍‬ 250) or NHLs (n ‫؍‬ 232) were found to contain a single large T antigen-positive cell, whereas 2 positive controls were repeatedly stained. Therefore, our results do not support the hypothesis that SV40 is implicated in the etiology of human lymphomas.

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Section: Methodsmentioning

confidence: 99%

Immunohistochemical investigation of SV40 large T antigen in Hodgkin and non‐Hodgkin's lymphoma

Brousset

Araujo

Gascoyne

2004

Intl Journal of Cancer

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“…The staining of the positive control cell line was obtained after standard antigen retrieval and/or after amplification of the signal by CSA. One hepatocellular carcinoma cell line from T-Ag transgenic mice (Weber-Benarous et al, 1993) and a case of renal infection (Li et al, 2002) were used as positive controls. For negative controls (DNA negative), five tissue sections from T-Ag PCR-negative lung tumours and non-neoplastic tissues were used.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Detection of oncogenic virus genomes and gene products in lung carcinoma

et al. 2005

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We investigated a series of 122 cases of small cell lung carcinomas and non-small cell lung carcinomas for the presence of several viruses that are known to be oncogenic in humans. Thus, viral genomes (DNA) and/or RNA transcripts and/or proteins of human papillomaviruses (HPV) 16, 18, 31, 33, 51, Epstein -Barr virus (EBV), human herpesvirus 8 (HHV-8), human cytomegalovirus (HCMV) and simian virus 40 (SV40) were investigated on tissue sections (prepared in tissue microarrays) with different techniques of immunohistochemistry and in situ hybridisation. None of the cases displayed a single positive tumour cell for all the viruses tested whatever the technique applied. Of note, in five cases of tumours with lymphoid infiltrates, we detected scattered EBV (EBER)-positive bystander lymphocytes. In three cases, a faint nuclear staining was found with the anti-latent nuclear antigen/LANA1 (HHV-8) antibody. These cases were checked by PCR with two sets of primers (orf 26 and orf 75) and remained negative for this latter virus. Taken together, our data strongly suggest that the conventional human oncogenic viruses (HPV, EBV, HCMV, HHV-8 and SV40) are unlikely to play some role in the development of lung carcinomas.

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“…, hyperosmotic medium (Clegg et al 1986;Wheatley et al 1984Wheatley et al , 1990, polyols (Nagata et al 1989;Lin et al 1991), weak bases (Ohkuma & Poole 1981;Finnin et al 1969;Yang et al 1965), bacterial toxins (Schmitt & Haas 1994;Papini et al 1993;Agata et al 1994;Hughes et al 1988), ultrafiltrate of human sera (Henics et al 1993) or retroviral infection (Weber-Benarous et al 1993). In many of these cases, vacuolization occurs in a perinuclear position, which progresses towards the periphery, with increased vacuole number and size.…”

mentioning

confidence: 99%

Vacuolar cytoplasmic phase separation in cultured mammalian cells involves the microfilament network and reduces motional properties of intracellular water

Henics

Wheatley

1997

Int J Experimental Path

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Hep-2, human epithelial carcinoma cells, and human foreskin fibroblasts (FF9 and FF13) were exposed to either an ultrafiltrate (< 50 kD) of human sera or the weak base, procaine hydrochloride, to induce reversible cytoplasmic vacuolization. The formation of vacuoles was shown not to be due to imbibition of medium. Ultrastructural details obtained from various stages of vacuole formation were compared. In both cases of induction vacuoles were irregular and often appeared membraneless, with little in the way of electron-dense content. They started to form in the perinuclear cytoplasm and progressed towards the periphery. Osmotic stress was not involved since mitochondria remained normal throughout a vacuolization episode. Vacuoles were often seen in close contact with filamentous structures, and this association remained detectable at late stages of the phenomenon. Fluorescent visualization of F-actin confirmed that the vacuoles were frequently bordered by microfilaments. No major metabolic impairment was apparent in vacuolized cells as judged by protein synthesis measurements, but nuclear fluorescence (DNA content) and forward light scatter (nuclear volume) by flow cytometric analysis suggested late S phase and G2 retardation. 1H-nmr relaxation measurements indicated intracellular water restricted in motional characteristics in vacuolized cells. The possibility of a restricted cytoplasmic phase separation as part of a transient adaptation response is raised, and a hypothesis to explain the findings is discussed.

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Retroviral Infection of Primary Hepatocytes from Normal Mice and Mice Transgenic for SV40 Large T Antigen

Cited by 17 publications

References 0 publications

Immunohistochemical investigation of SV40 large T antigen in Hodgkin and non‐Hodgkin's lymphoma

Immunohistochemical investigation of SV40 large T antigen in Hodgkin and non‐Hodgkin's lymphoma

Detection of oncogenic virus genomes and gene products in lung carcinoma

Vacuolar cytoplasmic phase separation in cultured mammalian cells involves the microfilament network and reduces motional properties of intracellular water

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